Escherichia coli K-12 substr. MG1655 Polypeptide: L-methionine / D-methionine ABC transporter - membrane subunit

Gene: metI Accession Numbers: EG11737 (EcoCyc), b0198, ECK0198

Synonyms: metD, yaeE

Regulation Summary Diagram: ?

Regulation summary diagram for metI

Component of: L-methionine / D-methionine ABC transporter (extended summary available)

membrane component of ABC transporter

Gene Citations: [Merlin02]

Locations: inner membrane

Map Position: [220,968 <- 221,621] (4.76 centisomes, 17°)
Length: 654 bp / 217 aa

Molecular Weight of Polypeptide: 23.256 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0000668 , DIP:DIP-11198N , EchoBASE:EB1688 , EcoGene:EG11737 , EcoliWiki:b0198 , OU-Microarray:b0198 , PortEco:metI , PR:PRO_000023214 , Protein Model Portal:P31547 , RefSeq:NP_414740 , RegulonDB:EG11737 , SMR:P31547 , String:511145.b0198 , UniProt:P31547

Relationship Links: InterPro:IN-FAMILY:IPR000515 , PDB:Structure:3DHW , PDB:Structure:3TUI , PDB:Structure:3TUJ , PDB:Structure:3TUZ , Pfam:IN-FAMILY:PF00528 , Prosite:IN-FAMILY:PS50928

In Paralogous Gene Group: 24 (43 members)

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Genetic Regulation Schematic: ?

Genetic regulation schematic for metI

GO Terms:

Biological Process: GO:0048473 - D-methionine transport Inferred from experiment [Merlin02]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0006865 - amino acid transport Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0005515 - protein binding Inferred from experiment [Johnson12, Kadaba08]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Zhang07, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a]

MultiFun Terms: cell structure membrane
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily, membrane component

Essentiality data for metI knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of: L-methionine / D-methionine ABC transporter

Subunit composition of L-methionine / D-methionine ABC transporter = [MetN]2[MetI][MetQ]
         L-methionine / D-methionine ABC transporter - ATP binding subunit = MetN (summary available)
         L-methionine / D-methionine ABC transporter - membrane subunit = MetI (summary available)
         L-methionine / D-methionine ABC transporter - periplasmic binding protein = MetQ (summary available)

The formerly designated abc-yaeE-yaeC putative ABC transporter gene cluster (now renamed metNIQ) has been shown to be involved in the uptake of D-methionine in E. coli K-12.

Deletion of the cluster resulted in a strain which was completely unable to grow in minimal media with D-methionine present. Complementation studies showed that nothing less than the full complement of the three genes was able to utilize D-methionine indicating that all three are necessary for proper transport system function [Merlin02]. Based on sequence similarity, MetN is the putative ATPase, MetI is the membrane spanning region and MetQ is the substrate-binding domain [Merlin02]. MetQ is the major binding protein for both L- and D-methionine as well as their analogues such as N-formyl methionine. L-methionine effectively competes for D-methionine transport while D-methionine does not strongly compete with L-methionine transport. The difference in inhibition between the two isomers is due to the low Km for L-methionine which is 15-fold lower than that for D-methionine.

E.coli strains auxotrophic for methionine can utilise a number of compounds including methioninyl peptides, N-acetyl methionine and methionine sulfoxime. Methionine sulfoxide and methionine sulfoximine were shown to be effective inhibitors of D-methionine uptake while N-acetyl methionine inhibited transport of both D- and L-methionine, as did seleno-methionine [Kadner77].

The MetNIQ transport system represents a novel family in the ABC type permease superfamily. All membrane proteins within this family exhibit five putative alpha helical membrane spanners [Zhang03d].

The crystal structure of MetNIQ has been determined to 3.7 Å resolution [Kadaba08].

Enzymatic reaction of: L-methionine S-oxide transport (L-methionine / D-methionine ABC transporter)

Transport reaction diagram for L-methionine S-oxide transport

Enzymatic reaction of: N-acetyl-DL-methionine transport (L-methionine / D-methionine ABC transporter)

Transport reaction diagram for N-acetyl-DL-methionine transport

Enzymatic reaction of: methionine transport (L-methionine / D-methionine ABC transporter)

Synonyms: Transport of L-methionine

Transport reaction diagram for methionine transport

Inhibitors (Competitive): seleno-L-methionine [Kadner77]

Primary Physiological Regulators of Enzyme Activity: seleno-L-methionine

Enzymatic reaction of: methionine transport (L-methionine / D-methionine ABC transporter)

Synonyms: Transport of D-methionine

Transport reaction diagram for methionine transport

Sequence Features

Protein sequence of L-methionine / D-methionine ABC transporter - membrane subunit with features indicated

Feature Class Location Citations Comment
Conserved-Region 13 -> 204
UniProt: ABC transmembrane type-1;
Transmembrane-Region 20 -> 40
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 58 -> 78
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 81 -> 101
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 152 -> 172
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 186 -> 206
UniProt: Helical;; Non-Experimental Qualifier: potential;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


10/20/97 Gene b0198 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11737; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Johnson12: Johnson E, Nguyen PT, Yeates TO, Rees DC (2012). "Inward facing conformations of the MetNI methionine ABC transporter: Implications for the mechanism of transinhibition." Protein Sci 21(1);84-96. PMID: 22095702

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Kadaba08: Kadaba NS, Kaiser JT, Johnson E, Lee A, Rees DC (2008). "The high-affinity E. coli methionine ABC transporter: structure and allosteric regulation." Science 321(5886);250-3. PMID: 18621668

Kadner77: Kadner RJ (1977). "Transport and utilization of D-methionine and other methionine sources in Escherichia coli." J Bacteriol 129(1);207-16. PMID: 318639

Merlin02: Merlin C, Gardiner G, Durand S, Masters M (2002). "The Escherichia coli metD locus encodes an ABC transporter which includes Abc (MetN), YaeE (MetI), and YaeC (MetQ)." J Bacteriol 2002;184(19);5513-7. PMID: 12218041

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Zhang03d: Zhang Z, Feige JN, Chang AB, Anderson IJ, Brodianski VM, Vitreschak AG, Gelfand MS, Saier MH (2003). "A transporter of Escherichia coli specific for L- and D-methionine is the prototype for a new family within the ABC superfamily." Arch Microbiol 180(2);88-100. PMID: 12819857

Zhang07: Zhang N, Chen R, Young N, Wishart D, Winter P, Weiner JH, Li L (2007). "Comparison of SDS- and methanol-assisted protein solubilization and digestion methods for Escherichia coli membrane proteome analysis by 2-D LC-MS/MS." Proteomics 7(4);484-93. PMID: 17309111

Other References Related to Gene Regulation

Drazic13: Drazic A, Miura H, Peschek J, Le Y, Bach NC, Kriehuber T, Winter J (2013). "Methionine oxidation activates a transcription factor in response to oxidative stress." Proc Natl Acad Sci U S A 110(23);9493-8. PMID: 23690622

Gal02: Gal J, Szvetnik A, Schnell R, Kalman M (2002). "The metD D-methionine transporter locus of Escherichia coli is an ABC transporter gene cluster." J Bacteriol 2002;184(17);4930-2. PMID: 12169620

Gebendorfer12: Gebendorfer KM, Drazic A, Le Y, Gundlach J, Bepperling A, Kastenmuller A, Ganzinger KA, Braun N, Franzmann TM, Winter J (2012). "Identification of a Hypochlorite-specific Transcription Factor from Escherichia coli." J Biol Chem 287(9);6892-903. PMID: 22223481

Liu01: Liu R, Blackwell TW, States DJ (2001). "Conformational model for binding site recognition by the E.coli MetJ transcription factor." Bioinformatics 17(7);622-33. PMID: 11448880

Marincs06: Marincs F, Manfield IW, Stead JA, McDowall KJ, Stockley PG (2006). "Transcript analysis reveals an extended regulon and the importance of protein-protein co-operativity for the Escherichia coli methionine repressor." Biochem J 396(2);227-34. PMID: 16515535

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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