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Escherichia coli K-12 substr. MG1655 Polypeptide: galactose ABC transporter - ATP binding subunit



Gene: mglA Accession Numbers: EG10592 (EcoCyc), b2149, ECK2142

Synonyms: mglP, mgl

Regulation Summary Diagram: ?

Component of: methyl-β-D-galactoside / galactose ABC transporter (extended summary available)

Summary:
MglA is the ATP-binding component of the galactose ABC transporter.

Gene Citations: [Harayama83, Hogg91]

Locations: inner membrane, cytosol

Map Position: [2,235,791 <- 2,237,311] (48.19 centisomes)
Length: 1521 bp / 506 aa

Molecular Weight of Polypeptide: 56.415 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0007103 , CGSC:502 , EchoBASE:EB0587 , EcoGene:EG10592 , EcoliWiki:b2149 , ModBase:P0AAG8 , OU-Microarray:b2149 , PortEco:mglA , PR:PRO_000023222 , Pride:P0AAG8 , Protein Model Portal:P0AAG8 , RefSeq:NP_416654 , RegulonDB:EG10592 , SMR:P0AAG8 , String:511145.b2149 , UniProt:P0AAG8

Relationship Links: InterPro:IN-FAMILY:IPR003439 , InterPro:IN-FAMILY:IPR003593 , InterPro:IN-FAMILY:IPR015862 , InterPro:IN-FAMILY:IPR017871 , InterPro:IN-FAMILY:IPR027417 , Pfam:IN-FAMILY:PF00005 , Prosite:IN-FAMILY:PS00211 , Prosite:IN-FAMILY:PS50893 , Prosite:IN-FAMILY:PS51260 , Smart:IN-FAMILY:SM00382

In Paralogous Gene Group: 23 (75 members)

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0006974 - cellular response to DNA damage stimulus Inferred from experiment [Khil02]
GO:0015757 - galactose transport Inferred from experiment [Ordal74]
GO:0006200 - ATP catabolic process Inferred by computational analysis [GOA01]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
GO:0008152 - metabolic process Inferred by computational analysis [UniProtGOA11]
GO:0008643 - carbohydrate transport Inferred by computational analysis [UniProtGOA11, GOA06]
GO:0034219 - carbohydrate transmembrane transport Inferred by computational analysis [GOA01]
Molecular Function: GO:0005354 - galactose transmembrane transporter activity Inferred from experiment Inferred by computational analysis [GOA06, Ordal74]
GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11, GOA06, GOA01]
GO:0015407 - monosaccharide-transporting ATPase activity Inferred by computational analysis [GOA01a]
GO:0016787 - hydrolase activity Inferred by computational analysis [UniProtGOA11]
GO:0016887 - ATPase activity Inferred by computational analysis [GOA01]
GO:0043211 - carbohydrate-transporting ATPase activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, DiazMejia09, Harayama83]
GO:0005829 - cytosol
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0043190 - ATP-binding cassette (ABC) transporter complex Inferred by computational analysis [GOA06]

MultiFun Terms: metabolism carbon utilization carbon compounds
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily ATP binding cytoplasmic component

Essentiality data for mglA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of: methyl-β-D-galactoside / galactose ABC transporter

Synonyms: MglBAC

Subunit composition of methyl-β-D-galactoside / galactose ABC transporter = [MglA]2[MglC]2[MglB]
         galactose ABC transporter - ATP binding subunit = MglA (summary available)
         galactose ABC transporter - membrane subunit = MglC (summary available)
         galactose ABC transporter - periplasmic binding protein = MglB (summary available)

Summary:
MglBAC is a beta-methylgalactoside transport system that is a member of the ATP-Binding Cassette (ABC) Superfamily of transporters [Wu95]. Based on sequence similarity, mglB encodes the galactose-binding component, mglC encodes the integral membrane component, and mglA encodes the ATP-binding component of the ABC transporter. Insertional mutations in each gene indicate that all three components are necessary for beta-methylgalactoside transport function [Harayama83]. Complementation experiments show that mlg genes cloned into a plasmid vector are able to complement the transport functions of the mglA, mglB, and mglC mutants [Rotman82]. MglB, but not MglA or MglC, was found to also serve as the galactose chemoreceptor in E. coli [Harayama83]. mglB mutants eliminate the chemotactic function in E. coli; however, mglA and MglC mutants exhibit normal galactose taxis but defective galactoside uptake activities [Scholle87, Harayama83].


Enzymatic reaction of: transport of methyl-β-D-galactoside (methyl-β-D-galactoside / galactose ABC transporter)


Enzymatic reaction of: transport of β-D-galactose (methyl-β-D-galactoside / galactose ABC transporter)

EC Number: 3.6.3.17


Sequence Features

Feature Class Location Citations Comment
Conserved-Region 14 -> 249
[UniProt09]
UniProt: ABC transporter 1;
Nucleotide-Phosphate-Binding-Region 46 -> 53
[UniProt10a]
UniProt: ATP; Non-Experimental Qualifier: potential;
Sequence-Conflict 57 -> 69
[Scholle87, UniProt10]
Alternate sequence: KCLFGIYQKDSGT → NACLVFIKRLRH; UniProt: (in Ref. 4);
Sequence-Conflict 163
[Blattner97, UniProt10]
Alternate sequence: A → G; UniProt: (in Ref. 2; AAA24170);
Conserved-Region 264 -> 506
[UniProt09]
UniProt: ABC transporter 2;


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
10/20/97 Gene b2149 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10592; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Blattner97: Blattner FR, Plunkett G, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y (1997). "The complete genome sequence of Escherichia coli K-12." Science 277(5331);1453-74. PMID: 9278503

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA01a: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Harayama83: Harayama S, Bollinger J, Iino T, Hazelbauer GL (1983). "Characterization of the mgl operon of Escherichia coli by transposon mutagenesis and molecular cloning." J Bacteriol 1983;153(1);408-15. PMID: 6294056

Hogg91: Hogg RW, Voelker C, Von Carlowitz I (1991). "Nucleotide sequence and analysis of the mgl operon of Escherichia coli K12." Mol Gen Genet 229(3);453-9. PMID: 1719366

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Khil02: Khil PP, Camerini-Otero RD (2002). "Over 1000 genes are involved in the DNA damage response of Escherichia coli." Mol Microbiol 44(1);89-105. PMID: 11967071

Ordal74: Ordal GW, Adler J (1974). "Properties of mutants in galactose taxis and transport." J Bacteriol 117(2);517-26. PMID: 4359648

Robbins75: Robbins AR, Rotman B (1975). "Evidence for binding protein-independent substrate translocation by the methylgalactoside transport system of Escherichia coli K12." Proc Natl Acad Sci U S A 72(2);423-7. PMID: 1091926

Robbins76: Robbins AR, Guzman R, Rotman B (1976). "Roles of individual mgl gene products in the beta-methylgalactoside transport system of Escherichia coli K12." J Biol Chem 251(10);3112-6. PMID: 773938

Rotman82: Rotman B, Guzman R (1982). "Identification of the mglA gene product in the beta-methylgalactoside transport system of Escherichia coli using plasmid DNA deletions generated in vitro." J Biol Chem 1982;257(15);9030-4. PMID: 6807987

Scholle87: Scholle A, Vreemann J, Blank V, Nold A, Boos W, Manson MD (1987). "Sequence of the mglB gene from Escherichia coli K12: comparison of wild-type and mutant galactose chemoreceptors." Mol Gen Genet 1987;208(1-2);247-53. PMID: 3302609

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Wu95: Wu LF, Mandrand-Berthelot MA (1995). "A family of homologous substrate-binding proteins with a broad range of substrate specificity and dissimilar biological functions." Biochimie 1995;77(9);744-50. PMID: 8789466

Other References Related to Gene Regulation

Geanacopoulos97: Geanacopoulos M, Adhya S (1997). "Functional characterization of roles of GalR and GalS as regulators of the gal regulon." J Bacteriol 1997;179(1);228-34. PMID: 8982002

Hollands07: Hollands K, Busby SJ, Lloyd GS (2007). "New targets for the cyclic AMP receptor protein in the Escherichia coli K-12 genome." FEMS Microbiol Lett 274(1);89-94. PMID: 17608696

Huerta03: Huerta AM, Collado-Vides J (2003). "Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals." J Mol Biol 333(2);261-78. PMID: 14529615

Jackson08: Jackson RJ, Binet MR, Lee LJ, Ma R, Graham AI, McLeod CW, Poole RK (2008). "Expression of the PitA phosphate/metal transporter of Escherichia coli is responsive to zinc and inorganic phosphate levels." FEMS Microbiol Lett 289(2);219-24. PMID: 19054109

NotleyMcRobb02: Notley-McRobb L, King T, Ferenci T (2002). "rpoS mutations and loss of general stress resistance in Escherichia coli populations as a consequence of conflict between competing stress responses." J Bacteriol 184(3);806-11. PMID: 11790751

Otsuka96: Otsuka J, Watanabe H, Mori KT (1996). "Evolution of transcriptional regulation system through promiscuous coupling of regulatory proteins with operons; suggestion from protein sequence similarities in Escherichia coli." J Theor Biol 1996;178(2);183-204. PMID: 8729576

Pruss01: Pruss BM, Liu X, Hendrickson W, Matsumura P (2001). "FlhD/FlhC-regulated promoters analyzed by gene array and lacZ gene fusions." FEMS Microbiol Lett 2001;197(1);91-7. PMID: 11287152

Raivio13: Raivio TL, Leblanc SK, Price NL (2013). "The Escherichia coli Cpx envelope stress response regulates genes of diverse function that impact antibiotic resistance and membrane integrity." J Bacteriol 195(12);2755-67. PMID: 23564175

Semsey07: Semsey S, Krishna S, Sneppen K, Adhya S (2007). "Signal integration in the galactose network of Escherichia coli." Mol Microbiol 65(2);465-76. PMID: 17630975

Weickert92: Weickert MJ, Adhya S (1992). "Isorepressor of the gal regulon in Escherichia coli." J Mol Biol 1992;226(1);69-83. PMID: 1619663

Weickert93: Weickert MJ, Adhya S (1993). "The galactose regulon of Escherichia coli." Mol Microbiol 1993;10(2);245-51. PMID: 7934815

Weickert93a: Weickert MJ, Adhya S (1993). "Control of transcription of gal repressor and isorepressor genes in Escherichia coli." J Bacteriol 1993;175(1);251-8. PMID: 8416900

Xu95: Xu J, Johnson RC (1995). "Identification of genes negatively regulated by Fis: Fis and RpoS comodulate growth-phase-dependent gene expression in Escherichia coli." J Bacteriol 177(4);938-47. PMID: 7860604

Zheng04: Zheng D, Constantinidou C, Hobman JL, Minchin SD (2004). "Identification of the CRP regulon using in vitro and in vivo transcriptional profiling." Nucleic Acids Res 32(19);5874-93. PMID: 15520470


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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