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Escherichia coli K-12 substr. MG1655 Polypeptide: galactose ABC transporter - membrane subunit



Gene: mglC Accession Numbers: EG10594 (EcoCyc), b2148, ECK2141

Synonyms: mglP

Regulation Summary Diagram: ?

Component of: methyl-β-D-galactoside / galactose ABC transporter (extended summary available)

Summary:
MglC is the integral membrane component of the galactose ABC transporter.

Gene Citations: [Harayama83, Hogg91]

Locations: inner membrane

Map Position: [2,234,765 <- 2,235,775] (48.17 centisomes)
Length: 1011 bp / 336 aa

Molecular Weight of Polypeptide: 35.55 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0007101 , CGSC:500 , EchoBASE:EB0589 , EcoGene:EG10594 , EcoliWiki:b2148 , OU-Microarray:b2148 , PortEco:mglC , PR:PRO_000023224 , Pride:P23200 , Protein Model Portal:P23200 , RefSeq:NP_416653 , RegulonDB:EG10594 , String:511145.b2148 , UniProt:P23200

Relationship Links: InterPro:IN-FAMILY:IPR001851 , Pfam:IN-FAMILY:PF02653

In Paralogous Gene Group: 189 (15 members)

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0006974 - cellular response to DNA damage stimulus Inferred from experiment [Khil02]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0008643 - carbohydrate transport Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0005215 - transporter activity Inferred by computational analysis [GOA01]
GO:0015407 - monosaccharide-transporting ATPase activity Inferred by computational analysis
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, DiazMejia09, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11]

MultiFun Terms: cell structure membrane
metabolism carbon utilization carbon compounds
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily, membrane component

Essentiality data for mglC knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]
Yes [Feist07, Comment 3]

Subunit of: methyl-β-D-galactoside / galactose ABC transporter

Synonyms: MglBAC

Subunit composition of methyl-β-D-galactoside / galactose ABC transporter = [MglA]2[MglC]2[MglB]
         galactose ABC transporter - ATP binding subunit = MglA (summary available)
         galactose ABC transporter - membrane subunit = MglC (summary available)
         galactose ABC transporter - periplasmic binding protein = MglB (summary available)

Summary:
MglBAC is a beta-methylgalactoside transport system that is a member of the ATP-Binding Cassette (ABC) Superfamily of transporters [Wu95]. Based on sequence similarity, mglB encodes the galactose-binding component, mglC encodes the integral membrane component, and mglA encodes the ATP-binding component of the ABC transporter. Insertional mutations in each gene indicate that all three components are necessary for beta-methylgalactoside transport function [Harayama83]. Complementation experiments show that mlg genes cloned into a plasmid vector are able to complement the transport functions of the mglA, mglB, and mglC mutants [Rotman82]. MglB, but not MglA or MglC, was found to also serve as the galactose chemoreceptor in E. coli [Harayama83]. mglB mutants eliminate the chemotactic function in E. coli; however, mglA and MglC mutants exhibit normal galactose taxis but defective galactoside uptake activities [Scholle87, Harayama83].


Enzymatic reaction of: transport of methyl-β-D-galactoside (methyl-β-D-galactoside / galactose ABC transporter)


Enzymatic reaction of: transport of β-D-galactose (methyl-β-D-galactoside / galactose ABC transporter)

EC Number: 3.6.3.17


Sequence Features

Feature Class Location Citations Comment
Transmembrane-Region 17 -> 37
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 53 -> 73
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 107 -> 127
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 181 -> 201
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 227 -> 247
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 257 -> 277
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 279 -> 299
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 306 -> 326
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
10/20/97 Gene b2148 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10594; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Harayama83: Harayama S, Bollinger J, Iino T, Hazelbauer GL (1983). "Characterization of the mgl operon of Escherichia coli by transposon mutagenesis and molecular cloning." J Bacteriol 1983;153(1);408-15. PMID: 6294056

Hogg91: Hogg RW, Voelker C, Von Carlowitz I (1991). "Nucleotide sequence and analysis of the mgl operon of Escherichia coli K12." Mol Gen Genet 229(3);453-9. PMID: 1719366

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Khil02: Khil PP, Camerini-Otero RD (2002). "Over 1000 genes are involved in the DNA damage response of Escherichia coli." Mol Microbiol 44(1);89-105. PMID: 11967071

Robbins75: Robbins AR, Rotman B (1975). "Evidence for binding protein-independent substrate translocation by the methylgalactoside transport system of Escherichia coli K12." Proc Natl Acad Sci U S A 72(2);423-7. PMID: 1091926

Robbins76: Robbins AR, Guzman R, Rotman B (1976). "Roles of individual mgl gene products in the beta-methylgalactoside transport system of Escherichia coli K12." J Biol Chem 251(10);3112-6. PMID: 773938

Rotman82: Rotman B, Guzman R (1982). "Identification of the mglA gene product in the beta-methylgalactoside transport system of Escherichia coli using plasmid DNA deletions generated in vitro." J Biol Chem 1982;257(15);9030-4. PMID: 6807987

Scholle87: Scholle A, Vreemann J, Blank V, Nold A, Boos W, Manson MD (1987). "Sequence of the mglB gene from Escherichia coli K12: comparison of wild-type and mutant galactose chemoreceptors." Mol Gen Genet 1987;208(1-2);247-53. PMID: 3302609

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Wu95: Wu LF, Mandrand-Berthelot MA (1995). "A family of homologous substrate-binding proteins with a broad range of substrate specificity and dissimilar biological functions." Biochimie 1995;77(9);744-50. PMID: 8789466

Other References Related to Gene Regulation

Geanacopoulos97: Geanacopoulos M, Adhya S (1997). "Functional characterization of roles of GalR and GalS as regulators of the gal regulon." J Bacteriol 1997;179(1);228-34. PMID: 8982002

Hollands07: Hollands K, Busby SJ, Lloyd GS (2007). "New targets for the cyclic AMP receptor protein in the Escherichia coli K-12 genome." FEMS Microbiol Lett 274(1);89-94. PMID: 17608696

Huerta03: Huerta AM, Collado-Vides J (2003). "Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals." J Mol Biol 333(2);261-78. PMID: 14529615

Jackson08: Jackson RJ, Binet MR, Lee LJ, Ma R, Graham AI, McLeod CW, Poole RK (2008). "Expression of the PitA phosphate/metal transporter of Escherichia coli is responsive to zinc and inorganic phosphate levels." FEMS Microbiol Lett 289(2);219-24. PMID: 19054109

NotleyMcRobb02: Notley-McRobb L, King T, Ferenci T (2002). "rpoS mutations and loss of general stress resistance in Escherichia coli populations as a consequence of conflict between competing stress responses." J Bacteriol 184(3);806-11. PMID: 11790751

Otsuka96: Otsuka J, Watanabe H, Mori KT (1996). "Evolution of transcriptional regulation system through promiscuous coupling of regulatory proteins with operons; suggestion from protein sequence similarities in Escherichia coli." J Theor Biol 1996;178(2);183-204. PMID: 8729576

Pruss01: Pruss BM, Liu X, Hendrickson W, Matsumura P (2001). "FlhD/FlhC-regulated promoters analyzed by gene array and lacZ gene fusions." FEMS Microbiol Lett 2001;197(1);91-7. PMID: 11287152

Raivio13: Raivio TL, Leblanc SK, Price NL (2013). "The Escherichia coli Cpx envelope stress response regulates genes of diverse function that impact antibiotic resistance and membrane integrity." J Bacteriol 195(12);2755-67. PMID: 23564175

Semsey07: Semsey S, Krishna S, Sneppen K, Adhya S (2007). "Signal integration in the galactose network of Escherichia coli." Mol Microbiol 65(2);465-76. PMID: 17630975

Weickert92: Weickert MJ, Adhya S (1992). "Isorepressor of the gal regulon in Escherichia coli." J Mol Biol 1992;226(1);69-83. PMID: 1619663

Weickert93: Weickert MJ, Adhya S (1993). "The galactose regulon of Escherichia coli." Mol Microbiol 1993;10(2);245-51. PMID: 7934815

Weickert93a: Weickert MJ, Adhya S (1993). "Control of transcription of gal repressor and isorepressor genes in Escherichia coli." J Bacteriol 1993;175(1);251-8. PMID: 8416900

Xu95b: Xu J, Johnson RC (1995). "Identification of genes negatively regulated by Fis: Fis and RpoS comodulate growth-phase-dependent gene expression in Escherichia coli." J Bacteriol 177(4);938-47. PMID: 7860604

Zheng04: Zheng D, Constantinidou C, Hobman JL, Minchin SD (2004). "Identification of the CRP regulon using in vitro and in vivo transcriptional profiling." Nucleic Acids Res 32(19);5874-93. PMID: 15520470


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 28, 2014, BIOCYC13A.