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Escherichia coli K-12 substr. MG1655 Protein: MalT-MalK

Subunit composition of MalT-MalK = [MalT][MalK]
         MalT transcriptional activator = MalT
         maltose ABC transporter - ATP binding subunit = MalK (summary available)

In Reactions of unknown directionality:

Not in pathways:
MalT + MalK = MalT-MalK

Gene-Reaction Schematic: ?


Subunit of MalT-MalK: MalT transcriptional activator

Synonyms: MalA, MalT

Gene: malT Accession Numbers: EG10562 (EcoCyc), b3418, ECK3405

Locations: cytosol

Sequence Length: 901 AAs

Molecular Weight: 103.12 kD (from nucleotide sequence)

pI: 6.3

GO Terms:

Biological Process: GO:0005975 - carbohydrate metabolic process Inferred by computational analysis [UniProtGOA11a]
GO:0006351 - transcription, DNA-templated Inferred by computational analysis [UniProtGOA11a]
GO:0006355 - regulation of transcription, DNA-templated Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0045893 - positive regulation of transcription, DNA-templated Inferred by computational analysis [GOA06]
GO:0045913 - positive regulation of carbohydrate metabolic process Inferred by computational analysis [GOA06]
Molecular Function: GO:0005515 - protein binding Inferred from experiment [Rajagopala14, Butland05, Joly02]
GO:0042802 - identical protein binding Inferred from experiment [Rajagopala14, Marquenet10]
GO:0048031 - trisaccharide binding Inferred from experiment [Danot10]
GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11a]
GO:0003677 - DNA binding Inferred by computational analysis [UniProtGOA11a, GOA06, GOA01a]
GO:0003700 - sequence-specific DNA binding transcription factor activity Inferred by computational analysis [GOA06, GOA01a]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11a, GOA06]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: information transfer RNA related Transcription related
metabolism carbon utilization carbon compounds
regulation genetic unit regulated operon
regulation type of regulation transcriptional level activator

Unification Links: DIP:DIP-10149N , EcoliWiki:b3418 , Mint:MINT-1233939 , ModBase:P06993 , PR:PRO_000023155 , Pride:P06993 , Protein Model Portal:P06993 , RefSeq:NP_417877 , SMR:P06993 , String:511145.b3418 , UniProt:P06993

Relationship Links: InterPro:IN-FAMILY:IPR000792 , InterPro:IN-FAMILY:IPR011990 , InterPro:IN-FAMILY:IPR011991 , InterPro:IN-FAMILY:IPR016032 , InterPro:IN-FAMILY:IPR023768 , InterPro:IN-FAMILY:IPR027417 , PDB:Structure:1HZ4 , Pfam:IN-FAMILY:PF00196 , Prints:IN-FAMILY:PR00038 , Prosite:IN-FAMILY:PS00622 , Prosite:IN-FAMILY:PS50043 , Smart:IN-FAMILY:SM00421

In Reactions of unknown directionality:

Not in pathways:
MalT + MalK = MalT-MalK
MalT + maltotriose + ATP = MalT-maltotriose-ATP

Citations: [Cole86, Richet89]

Essentiality data for malT knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Subunit of MalT-MalK: maltose ABC transporter - ATP binding subunit

Synonyms: MalB

Gene: malK Accession Numbers: EG10558 (EcoCyc), b4035, ECK4027

Locations: inner membrane

Sequence Length: 371 AAs

Molecular Weight: 40.99 kD (from nucleotide sequence)

Molecular Weight: 43.0 kD (experimental) [Bavoil80]

GO Terms:

Biological Process: GO:0015768 - maltose transport Inferred from experiment Inferred by computational analysis [GOA01a, Davidson91]
GO:0042956 - maltodextrin transport Inferred from experiment [Ferenci80]
GO:0006200 - ATP catabolic process Inferred by computational analysis [GOA01a]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a]
GO:0008152 - metabolic process Inferred by computational analysis [UniProtGOA11a]
GO:0008643 - carbohydrate transport Inferred by computational analysis [UniProtGOA11a, GOA06]
GO:0055085 - transmembrane transport Inferred by computational analysis [GOA01a]
Molecular Function: GO:0015423 - maltose-transporting ATPase activity Inferred from experiment Inferred by computational analysis [GOA01, GOA01a, Davidson91]
GO:0015609 - maltooligosaccharide-importing ATPase activity Inferred from experiment Inferred by computational analysis [GOA06, Ferenci80]
GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11a]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11a, GOA06, GOA01a]
GO:0016787 - hydrolase activity Inferred by computational analysis [UniProtGOA11a]
GO:0016820 - hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances Inferred by computational analysis [GOA01a]
GO:0016887 - ATPase activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0016020 - membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, GOA01a, Wagner08]
GO:0043190 - ATP-binding cassette (ABC) transporter complex Inferred from experiment Inferred by computational analysis [GOA06, GOA01a, Davidson91]
GO:0005886 - plasma membrane Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, Bavoil80]

MultiFun Terms: metabolism carbon utilization carbon compounds
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily ATP binding cytoplasmic component

Unification Links: DIP:DIP-47850N , EcoliWiki:b4035 , Mint:MINT-1223477 , ModBase:P68187 , PR:PRO_000023151 , Pride:P68187 , Protein Model Portal:P68187 , RefSeq:NP_418459 , SMR:P68187 , String:511145.b4035 , UniProt:P68187

Relationship Links: InterPro:IN-FAMILY:IPR003439 , InterPro:IN-FAMILY:IPR003593 , InterPro:IN-FAMILY:IPR008995 , InterPro:IN-FAMILY:IPR012340 , InterPro:IN-FAMILY:IPR013611 , InterPro:IN-FAMILY:IPR015855 , InterPro:IN-FAMILY:IPR017871 , InterPro:IN-FAMILY:IPR027417 , PDB:Structure:1q12 , PDB:Structure:1q1b , PDB:Structure:1Q1E , PDB:Structure:2awn , PDB:Structure:2awo , PDB:Structure:2R6G , PDB:Structure:3FH6 , PDB:Structure:3GD7 , PDB:Structure:3PUV , PDB:Structure:3PUW , PDB:Structure:3PUX , PDB:Structure:3PUY , PDB:Structure:3PUZ , PDB:Structure:3PV0 , PDB:Structure:3RLF , PDB:Structure:4JBW , Pfam:IN-FAMILY:PF00005 , Pfam:IN-FAMILY:PF08402 , Prosite:IN-FAMILY:PS00211 , Prosite:IN-FAMILY:PS50893 , Prosite:IN-FAMILY:PS51245 , Smart:IN-FAMILY:SM00382

In Reactions of unknown directionality:

Not in pathways:
MalT + MalK = MalT-MalK

Summary:
MalK is the ATP-binding component of the maltose ABC transporter.

The structure of MalK has been determined to 2.6 Å resolution [Chen03]. The dynamics of MalK have been analysed using normal mode analysis and residues involved in conformational change identified [Lukman09].

Essentiality data for malK knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Bavoil80: Bavoil P, Hofnung M, Nikaido H (1980). "Identification of a cytoplasmic membrane-associated component of the maltose transport system of Escherichia coli." J Biol Chem 255(18);8366-9. PMID: 6997295

Butland05: Butland G, Peregrin-Alvarez JM, Li J, Yang W, Yang X, Canadien V, Starostine A, Richards D, Beattie B, Krogan N, Davey M, Parkinson J, Greenblatt J, Emili A (2005). "Interaction network containing conserved and essential protein complexes in Escherichia coli." Nature 433(7025);531-7. PMID: 15690043

Chen03: Chen J, Lu G, Lin J, Davidson AL, Quiocho FA (2003). "A tweezers-like motion of the ATP-binding cassette dimer in an ABC transport cycle." Mol Cell 12(3);651-61. PMID: 14527411

Cole86: Cole ST, Raibaud O (1986). "The nucleotide sequence of the malT gene encoding the positive regulator of the Escherichia coli maltose regulon." Gene 1986;42(2);201-8. PMID: 3015733

Danot10: Danot O (2010). "The inducer maltotriose binds in the central cavity of the tetratricopeptide-like sensor domain of MalT, a bacterial STAND transcription factor." Mol Microbiol 77(3);628-41. PMID: 20545845

Davidson91: Davidson AL, Nikaido H (1991). "Purification and characterization of the membrane-associated components of the maltose transport system from Escherichia coli." J Biol Chem 266(14);8946-51. PMID: 2026607

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Ferenci80: Ferenci T (1980). "The recognition of maltodextrins by Escherichia coli." Eur J Biochem 108(2);631-6. PMID: 6997044

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Joly02: Joly N, Danot O, Schlegel A, Boos W, Richet E (2002). "The Aes protein directly controls the activity of MalT, the central transcriptional activator of the Escherichia coli maltose regulon." J Biol Chem 277(19);16606-13. PMID: 11867639

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Lukman09: Lukman S, Grant GH (2009). "A network of dynamically conserved residues deciphers the motions of maltose transporter." Proteins 76(3);588-97. PMID: 19274733

Marquenet10: Marquenet E, Richet E (2010). "Conserved motifs involved in ATP hydrolysis by MalT, a signal transduction ATPase with numerous domains from Escherichia coli." J Bacteriol 192(19);5181-91. PMID: 20693326

Rajagopala14: Rajagopala SV, Sikorski P, Kumar A, Mosca R, Vlasblom J, Arnold R, Franca-Koh J, Pakala SB, Phanse S, Ceol A, Hauser R, Siszler G, Wuchty S, Emili A, Babu M, Aloy P, Pieper R, Uetz P (2014). "The binary protein-protein interaction landscape of Escherichia coli." Nat Biotechnol 32(3);285-90. PMID: 24561554

Richet89: Richet E, Raibaud O (1989). "MalT, the regulatory protein of the Escherichia coli maltose system, is an ATP-dependent transcriptional activator." EMBO J 1989;8(3);981-7. PMID: 2524384

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Wagner08: Wagner S, Pop OI, Pop O, Haan GJ, Baars L, Koningstein G, Klepsch MM, Genevaux P, Luirink J, de Gier JW (2008). "Biogenesis of MalF and the MalFGK(2) maltose transport complex in Escherichia coli requires YidC." J Biol Chem 283(26);17881-90. PMID: 18456666


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sat Dec 20, 2014, biocyc11.