Escherichia coli K-12 substr. MG1655 Enzyme: KpLE2 phage-like element; IS30 transposase

Gene: insI-3 Accession Numbers: G7905 (EcoCyc), b4284, ECK0258

Synonyms: tra8-3

Regulation Summary Diagram: ?

Regulation summary diagram for insI-3

Locations: cytosol

Map Position: [4,505,489 <- 4,506,640] (97.11 centisomes, 350°)
Length: 1152 bp / 383 aa

Molecular Weight of Polypeptide: 44.281 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0014045 , EcoGene:EG60051 , EcoliWiki:b4284 , OU-Microarray:b4284 , PortEco:insI-3 , Pride:P0CF90 , Protein Model Portal:P0CF90 , RefSeq:NP_418704 , RegulonDB:G7905 , SMR:P0CF90 , UniProt:P0CF90

Relationship Links: InterPro:IN-FAMILY:IPR001584 , InterPro:IN-FAMILY:IPR001598 , InterPro:IN-FAMILY:IPR012337 , InterPro:IN-FAMILY:IPR025246 , Pfam:IN-FAMILY:PF00665 , Pfam:IN-FAMILY:PF13936 , Prosite:IN-FAMILY:PS01043 , Prosite:IN-FAMILY:PS50994

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Biological Process: GO:0006310 - DNA recombination Inferred by computational analysis [UniProtGOA11]
GO:0006313 - transposition, DNA-mediated Inferred by computational analysis [GOA01]
GO:0015074 - DNA integration Inferred by computational analysis [GOA01]
GO:0032196 - transposition Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0003676 - nucleic acid binding Inferred by computational analysis [GOA01]
GO:0003677 - DNA binding Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0004803 - transposase activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: extrachromosomal transposon related

Essentiality data for insI-3 knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]

Created 07-Mar-2012 by Keseler I , SRI International

Enzymatic reaction of: transposase

EC Number: 2.7.7.-

an insertion sequence element IS30 <=> an insertion sequence IS30 with integrated transposon

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Reversibility of this reaction is unspecified.

Sequence Features

Protein sequence of KpLE2 phage-like element; IS30 transposase with features indicated

Feature Class Location Citations Comment
Conserved-Region 213 -> 379
UniProt: Integrase catalytic.

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

UniProt12b: UniProt Consortium (2012). "UniProt version 2012-02 released on 2012-02-29 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Tue Oct 6, 2015, biocyc13.