|Gene:||yneM||Accession Numbers: G0-8892 (EcoCyc), b4599, ECK4409|
YneM contains a predicted transmembrane segment, and the protein can be found in the membrane fraction [Hemm, 2008]. Using a sucrose fractionation protocol the majority of YneM partitions into the inner membrane fraction [Fontaine, 2011]. Topology assays using GFP and PhoA fusion proteins suggest that YneM is oriented with the C-terminus in the cytoplasm [Fontaine, 2011].
yneM mRNA has been detected [Wassarman01]. yneM is expressed during stationary phase (data not shown) [Hemm, 2008]. Expression of yneM is lower in minimal glucose medium than in rich medium, increases upon heat shock and SDS/EDTA treatment, and decreases upon diamide treatment [Hemm10].
Gene Citations: [Moon09]
Locations: inner membrane
|Map Position: [1,620,670 -> 1,620,765] (34.93 centisomes, 126°)||Length: 96 bp / 31 aa|
Molecular Weight of Polypeptide: 3.509 kD (from nucleotide sequence)
|Cellular Component:||GO:0005886 - plasma membrane
[UniProtGOA11a, UniProtGOA11, Fontaine, 2011]
GO:0016020 - membrane [UniProtGOA11, Hemm, 2008]
GO:0016021 - integral component of membrane [UniProtGOA11]
Hemm, 2008: Hemm MR, Paul BJ, Schneider TD, Storz G, Rudd KE (2008). "Small membrane proteins found by comparative genomics and ribosome binding site models." Mol Microbiol 70(6);1487-501. PMID: 19121005
Hemm10: Hemm MR, Paul BJ, Miranda-Rios J, Zhang A, Soltanzad N, Storz G (2010). "Small Stress Response Proteins in Escherichia coli: Proteins Missed by Classical Proteomic Studies." J Bacteriol 192(1):46-58. PMID: 19734316
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