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Escherichia coli K-12 substr. MG1655 Polypeptide: small membrane protein



Gene: yneM Accession Numbers: G0-8892 (EcoCyc), b4599, ECK4409

Regulation Summary Diagram: ?

Summary:
YneM contains a predicted transmembrane segment, and the protein can be found in the membrane fraction [Hemm08]. Using a sucrose fractionation protocol the majority of YneM partitions into the inner membrane fraction [Fontaine11]. Topology assays using GFP and PhoA fusion proteins suggest that YneM is oriented with the C-terminus in the cytoplasm [Fontaine11].

yneM mRNA has been detected [Wassarman01]. yneM is expressed during stationary phase (data not shown) [Hemm08]. Expression of yneM is lower in minimal glucose medium than in rich medium, increases upon heat shock and SDS/EDTA treatment, and decreases upon diamide treatment [Hemm10].

Gene Citations: [Moon09]

Locations: inner membrane

Map Position: [1,620,670 -> 1,620,765] (34.93 centisomes)
Length: 96 bp / 31 aa

Molecular Weight of Polypeptide: 3.509 kD (from nucleotide sequence)

Unification Links: EcoGene:EG14395 , EcoliWiki:b4599 , PortEco:yneM , RegulonDB:G0-8892 , String:511145.b4599 , UniProt:A5A616

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0006950 - response to stress Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, Fontaine11]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, Hemm08]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a]

Gene Class: ORFs

Credits:
Curated 21-Jan-2010 by Keseler I , SRI International
Last-Curated ? 16-Aug-2011 by Mackie A , Macquarie University


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:


References

Fontaine11: Fontaine F, Fuchs RT, Storz G (2011). "Membrane localization of small proteins in Escherichia coli." J Biol Chem 286(37);32464-74. PMID: 21778229

Hemm08: Hemm MR, Paul BJ, Schneider TD, Storz G, Rudd KE (2008). "Small membrane proteins found by comparative genomics and ribosome binding site models." Mol Microbiol 70(6);1487-501. PMID: 19121005

Hemm10: Hemm MR, Paul BJ, Miranda-Rios J, Zhang A, Soltanzad N, Storz G (2010). "Small Stress Response Proteins in Escherichia coli: Proteins Missed by Classical Proteomic Studies." J Bacteriol 192(1):46-58. PMID: 19734316

Moon09: Moon K, Gottesman S (2009). "A PhoQ/P-Regulated small RNA Regulates Sensitivity of Escherichia coli to Antimicrobial Peptides." Mol Microbiol 74(6):1314-30. PMID: 19889087

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Wassarman01: Wassarman KM, Repoila F, Rosenow C, Storz G, Gottesman S (2001). "Identification of novel small RNAs using comparative genomics and microarrays." Genes Dev 15(13);1637-51. PMID: 11445539


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 28, 2014, BIOCYC13B.