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Escherichia coli K-12 substr. MG1655 Transporter: N-acetylglucosamine PTS permease



Gene: nagE Accession Numbers: EG10635 (EcoCyc), b0679, ECK0667

Synonyms: pstN, ptsN, EIINag, Enzyme II N-acetyl glucosamine, EIICBANag

Regulation Summary Diagram: ?

Subunit composition of N-acetylglucosamine PTS permease = [NagE]2

Summary:
NagE, the N-acetylglucosamine PTS permease, belongs to the functional superfamily of the phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system (PTSsugar). The PTSsugar transports and simultaneously phosphorylates it's sugar substrates in a process called group translocation (reviewed in [Postma93]. NagE takes up exogenous N-acetylglucosamine, releasing the phosphate ester into the cell cytoplasm in preparation for metabolism, primarily via glycolysis [White70, Peri90] (see N-acetylglucosamine degradation I).

NagE, the Enzyme IINag complex, possesses three domains in a single polypeptide chain with the domain order IIC-IIB-IIA [Peri88]. It is homologous to PtsG/Crr (the glucose-specific PTS Enzyme II) [Peri88]. Hydropathy and sequence analysis suggests that the N-terminal region of NagE resides in the membrane. Histidine at position 569 and cysteine at position 412 are believed to be the sites of phosphorylation [Peri88, Rogers88, Postma93]. NagE is a member of the PTS Glucose-Glucoside family of transporters [Saier14].

The overall PTS-mediated phosphoryl transfer reaction, requiring the two general energy coupling proteins of the PTS, Enzyme I (PtsI) and HPr (PtsH), as well as the three domains of the Enzyme IINag complex can be represented as:

PEP → Enzyme I-Phis189 → HPr-Phis15 → Enzyme IIA-Phis569 → Enzyme IIB-Pcys412-(Enzyme IIC) → N-acetylglucosamine-6-P.

NagE transports N-acetylglucosamine with low micromolar affinity. It can also transport the antibiotic streptozotocin [Ammer79, Lengeler80]. Genetic studies suggest that the NagE PTS transporter plays a significant role in the recycling of peptidoglycan [Plumbridge09].

Expression of the nagE monocistronic operon is regulated by NagC together with the cyclic AMP-cyclic AMP receptor protein (CRP) complex [Plumbridge91, Plumbridge95].

Gene Citations: [Plumbridge91a]

Locations: inner membrane

Map Position: [703,167 -> 705,113] (15.16 centisomes)
Length: 1947 bp / 648 aa

Molecular Weight of Polypeptide: 68.347 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0002312 , CGSC:38380 , EchoBASE:EB0629 , EcoGene:EG10635 , EcoliWiki:b0679 , ModBase:P09323 , OU-Microarray:b0679 , PortEco:nagE , PR:PRO_000023340 , Pride:P09323 , Protein Model Portal:P09323 , RefSeq:NP_415205 , RegulonDB:EG10635 , SMR:P09323 , String:511145.b0679 , Swiss-Model:P09323 , UniProt:P09323

Relationship Links: InterPro:IN-FAMILY:IPR001127 , InterPro:IN-FAMILY:IPR001996 , InterPro:IN-FAMILY:IPR003352 , InterPro:IN-FAMILY:IPR010974 , InterPro:IN-FAMILY:IPR011055 , InterPro:IN-FAMILY:IPR011535 , InterPro:IN-FAMILY:IPR013013 , InterPro:IN-FAMILY:IPR018113 , Pfam:IN-FAMILY:PF00358 , Pfam:IN-FAMILY:PF00367 , Pfam:IN-FAMILY:PF02378 , Prosite:IN-FAMILY:PS00371 , Prosite:IN-FAMILY:PS01035 , Prosite:IN-FAMILY:PS51093 , Prosite:IN-FAMILY:PS51098 , Prosite:IN-FAMILY:PS51103

In Paralogous Gene Group: 176 (4 members) , 177 (2 members)

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0006974 - cellular response to DNA damage stimulus Inferred from experiment [Khil02]
GO:0009254 - peptidoglycan turnover Inferred from experiment [Plumbridge09]
GO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from experiment Inferred by computational analysis [UniProtGOA11a, GOA01a, Peri88]
GO:0015764 - N-acetylglucosamine transport Inferred from experiment [White70]
GO:0015771 - trehalose transport Inferred from experiment [Peri88]
GO:0042891 - antibiotic transport Inferred from experiment [Lengeler80]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a]
GO:0008643 - carbohydrate transport Inferred by computational analysis [UniProtGOA11a]
GO:0016310 - phosphorylation Inferred by computational analysis [UniProtGOA11a]
GO:0034219 - carbohydrate transmembrane transport Inferred by computational analysis [GOA01, GOA01a]
Molecular Function: GO:0042895 - antibiotic transporter activity Inferred from experiment [Lengeler80]
GO:0090586 - protein-phosphocysteine-N-acetylglucosamine phosphotransferase system transporter activity Author statement Inferred from experiment [Peri88, Postma93]
GO:0090587 - protein-phosphocysteine-glucosamine phosphotransferase system transporter activity Inferred from experiment [Lengeler80]
GO:0008982 - protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred by computational analysis [GOA01, GOA01a]
GO:0015572 - N-acetylglucosamine transmembrane transporter activity Inferred by computational analysis [GOA01a]
GO:0016301 - kinase activity Inferred by computational analysis [UniProtGOA11a]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005886 - plasma membrane Inferred by computational analysis [UniProtGOA11, UniProtGOA11a]
GO:0005887 - integral component of plasma membrane Inferred by computational analysis [Rogers88]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0019866 - organelle inner membrane Inferred by computational analysis [GOA01a]

MultiFun Terms: cell structure membrane
metabolism carbon utilization amines
metabolism central intermediary metabolism amino sugar conversions
transport Group Translocators Phosphotransferase Systems (PEP-dependent PTS)

Essentiality data for nagE knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Credits:
Last-Curated ? 03-Jul-2013 by Mackie A , Macquarie University


Enzymatic reaction of: N-acetylglucosamine PTS permease

Synonyms: Transport of N-acetyl-D-glucosamine


Enzymatic reaction of: D-glucosamine PTS permease (N-acetylglucosamine PTS permease)


Sequence Features

Feature Class Location Citations Comment
Conserved-Region 1 -> 371
[UniProt09]
UniProt: PTS EIIC type-1;
Transmembrane-Region 16 -> 36
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 38 -> 58
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 70 -> 90
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 92 -> 112
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 132 -> 152
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 159 -> 179
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 192 -> 212
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 232 -> 252
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 260 -> 280
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 282 -> 302
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 303 -> 323
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 339 -> 359
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Conserved-Region 390 -> 472
[UniProt09]
UniProt: PTS EIIB type-1;
Active-Site 412
[UniProt10a]
UniProt: Phosphocysteine intermediate; for EIIB activity; Non-Experimental Qualifier: by similarity;
Conserved-Region 517 -> 621
[UniProt09]
UniProt: PTS EIIA type-1;
Active-Site 569
[UniProt10a]
UniProt: Tele-phosphohistidine intermediate; for EIIA activity; Non-Experimental Qualifier: by similarity;


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
10/20/97 Gene b0679 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10635; confirmed by SwissProt match.


References

Ammer79: Ammer J, Brennenstuhl M, Schindler P, Holtje JV, Zahner H (1979). "Phosphorylation of streptozotocin during uptake via the phosphoenolpyruvate: sugar phosphotransferase system in Escherichia coli." Antimicrob Agents Chemother 16(6);801-7. PMID: 161156

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Khil02: Khil PP, Camerini-Otero RD (2002). "Over 1000 genes are involved in the DNA damage response of Escherichia coli." Mol Microbiol 44(1);89-105. PMID: 11967071

Lengeler80: Lengeler J (1980). "Characterisation of mutants of Escherichia coli K12, selected by resistance to streptozotocin." Mol Gen Genet 179(1);49-54. PMID: 6450313

Peri88: Peri KG, Waygood EB (1988). "Sequence of cloned enzyme IIN-acetylglucosamine of the phosphoenolpyruvate:N-acetylglucosamine phosphotransferase system of Escherichia coli." Biochemistry 1988;27(16);6054-61. PMID: 3056518

Peri90: Peri KG, Goldie H, Waygood EB (1990). "Cloning and characterization of the N-acetylglucosamine operon of Escherichia coli." Biochem Cell Biol 68(1);123-37. PMID: 2190615

Plumbridge09: Plumbridge J (2009). "An alternative route for recycling of N-acetylglucosamine from peptidoglycan involves the N-acetylglucosamine phosphotransferase system in Escherichia coli." J Bacteriol 191(18);5641-7. PMID: 19617367

Plumbridge91: Plumbridge JA (1991). "Repression and induction of the nag regulon of Escherichia coli K-12: the roles of nagC and nagA in maintenance of the uninduced state." Mol Microbiol 1991;5(8);2053-62. PMID: 1766379

Plumbridge91a: Plumbridge J, Kolb A (1991). "CAP and Nag repressor binding to the regulatory regions of the nagE-B and manX genes of Escherichia coli." J Mol Biol 1991;217(4);661-79. PMID: 1848637

Plumbridge95: Plumbridge J, Kolb A (1995). "Nag repressor-operator interactions: protein-DNA contacts cover more than two turns of the DNA helix." J Mol Biol 1995;249(5);890-902. PMID: 7791215

Postma93: Postma PW, Lengeler JW, Jacobson GR (1993). "Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria." Microbiol Rev 57(3);543-94. PMID: 8246840

Rogers88: Rogers MJ, Ohgi T, Plumbridge J, Soll D (1988). "Nucleotide sequences of the Escherichia coli nagE and nagB genes: the structural genes for the N-acetylglucosamine transport protein of the bacterial phosphoenolpyruvate: sugar phosphotransferase system and for glucosamine-6-phosphate deaminase." Gene 1988;62(2);197-207. PMID: 3284790

Saier14: Saier MH, Reddy VS, Tamang DG, Vastermark A (2014). "The transporter classification database." Nucleic Acids Res 42(1);D251-8. PMID: 24225317

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

White70: White RJ (1970). "The role of the phosphoenolpyruvate phosphotransferase system in the transport of N-acetyl-D-glucosamine by Escherichia coli." Biochem J 118(1);89-92. PMID: 4919472

Other References Related to Gene Regulation

Plumbridge01: Plumbridge J (2001). "DNA binding sites for the Mlc and NagC proteins: regulation of nagE, encoding the N-acetylglucosamine-specific transporter in Escherichia coli." Nucleic Acids Res 29(2);506-14. PMID: 11139621

Plumbridge93: Plumbridge J, Kolb A (1993). "DNA loop formation between Nag repressor molecules bound to its two operator sites is necessary for repression of the nag regulon of Escherichia coli in vivo." Mol Microbiol 10(5);973-81. PMID: 7934873

Plumbridge98a: Plumbridge J, Kolb A (1998). "DNA bending and expression of the divergent nagE-B operons." Nucleic Acids Res 26(5);1254-60. PMID: 9469834


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Dec 19, 2014, BIOCYC14A.