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Escherichia coli K-12 substr. MG1655 Polypeptide: NarP



Gene: narP Accession Numbers: EG11527 (EcoCyc), b2193, ECK2185

Synonyms: nitrate/nitrite response regulator NarP

Regulation Summary Diagram: ?

Alternative forms of NarP: NarP-Phosphorylated DNA-binding transcriptional dual regulator (extended summary available)

Summary:

Citations: [Pao95, Parkinson92, Stock90, Parkinson93, Rabin93, Darwin97, Browning05]

Locations: cytosol

Map Position: [2,288,522 -> 2,289,169] (49.33 centisomes)
Length: 648 bp / 215 aa

Molecular Weight of Polypeptide: 23.575 kD (from nucleotide sequence)

pI: 5.06

Unification Links: ASAP:ABE-0007259 , CGSC:32598 , DIP:DIP-10317N , EchoBASE:EB1489 , EcoGene:EG11527 , EcoliWiki:b2193 , ModBase:P31802 , OU-Microarray:b2193 , PortEco:narP , PR:PRO_000023357 , Pride:P31802 , Protein Model Portal:P31802 , RegulonDB:EG11527 , SMR:P31802 , String:511145.b2193 , Swiss-Model:P31802 , UniProt:P31802

Relationship Links: InterPro:IN-FAMILY:IPR000792 , InterPro:IN-FAMILY:IPR001789 , InterPro:IN-FAMILY:IPR011006 , InterPro:IN-FAMILY:IPR011991 , InterPro:IN-FAMILY:IPR016032 , Pfam:IN-FAMILY:PF00072 , Pfam:IN-FAMILY:PF00196 , Prints:IN-FAMILY:PR00038 , Prosite:IN-FAMILY:PS00622 , Prosite:IN-FAMILY:PS50043 , Prosite:IN-FAMILY:PS50110 , Smart:IN-FAMILY:SM00421 , Smart:IN-FAMILY:SM00448

In Paralogous Gene Group: 121 (40 members)

Reactions known to consume the compound:

NarQ Two-Component Signal Transduction System, nitrate dependent :
NarQ sensory histidine kinase - phosphorylated[inner membrane] + NarP → NarQ[inner membrane] + NarP-Pasp59

NarX Two-Component Signal Transduction System, nitrate dependent :
NarX sensory histidine kinase - phosphorylated[inner membrane] + NarP → NarX[inner membrane] + NarP-Pasp59

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0006355 - regulation of transcription, DNA-templated Inferred from experiment Inferred by computational analysis [UniProtGOA11a, GOA01a, Darwin97]
GO:0000160 - phosphorelay signal transduction system Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0006351 - transcription, DNA-templated Inferred by computational analysis [UniProtGOA11a]
GO:0042128 - nitrate assimilation Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0003677 - DNA binding Inferred from experiment Inferred by computational analysis [UniProtGOA11a, GOA01a, Darwin97]
GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11a]
GO:0003700 - sequence-specific DNA binding transcription factor activity Inferred by computational analysis [GOA01a]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: information transfer RNA related Transcription related
metabolism energy metabolism, carbon anaerobic respiration
regulation genetic unit regulated regulon
regulation type of regulation transcriptional level activator
regulation type of regulation transcriptional level complex regulation two component regulatory systems (external signal)
regulation type of regulation transcriptional level repressor

Transcription Units regulated by related protein NarP-Phosphorylated DNA-binding transcriptional dual regulator (12 total):

Essentiality data for narP knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes et al., 2003, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba et al., 2006, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba et al., 2006, Comment 2]

Sequence Features

Feature Class Location Citations Comment State
Conserved-Region 8 -> 124
[UniProt09]
UniProt: Response regulatory;
 
4-aspartylphosphate-Modification 59
[UniProt11]
UniProt: 4-aspartylphosphate; Non-Experimental Qualifier: by similarity.
 
Phosphorylation-Modification 59
[UniProt, 2010]
UniProt: 4-aspartylphosphate; Non-Experimental Qualifier: by similarity;
Unmodified
Conserved-Region 147 -> 212
[UniProt09]
UniProt: HTH luxR-type;
 
DNA-Binding-Region 171 -> 190
[UniProt, 2010]
UniProt: H-T-H motif; Non-Experimental Qualifier: by similarity;
 


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b2193 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11527; confirmed by SwissProt match.


References

Baba et al., 2006: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Browning05: Browning DF, Grainger DC, Beatty CM, Wolfe AJ, Cole JA, Busby SJ (2005). "Integration of three signals at the Escherichia coli nrf promoter: a role for Fis protein in catabolite repression." Mol Microbiol 57(2);496-510. PMID: 15978080

Darwin97: Darwin AJ, Tyson KL, Busby SJ, Stewart V (1997). "Differential regulation by the homologous response regulators NarL and NarP of Escherichia coli K-12 depends on DNA binding site arrangement." Mol Microbiol 1997;25(3);583-95. PMID: 9302020

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes et al., 2003: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Pao95: Pao GM, Saier MH (1995). "Response regulators of bacterial signal transduction systems: selective domain shuffling during evolution." J Mol Evol 1995;40(2);136-54. PMID: 7699720

Parkinson92: Parkinson JS, Kofoid EC (1992). "Communication modules in bacterial signaling proteins." Annu Rev Genet 1992;26;71-112. PMID: 1482126

Parkinson93: Parkinson JS (1993). "Signal transduction schemes of bacteria." Cell 1993;73(5);857-71. PMID: 8098993

Rabin93: Rabin RS, Stewart V (1993). "Dual response regulators (NarL and NarP) interact with dual sensors (NarX and NarQ) to control nitrate- and nitrite-regulated gene expression in Escherichia coli K-12." J Bacteriol 1993;175(11);3259-68. PMID: 8501030

Stock90: Stock JB, Stock AM, Mottonen JM (1990). "Signal transduction in bacteria." Nature 1990;344(6265);395-400. PMID: 2157156

UniProt, 2010: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt11: UniProt Consortium (2011). "UniProt version 2011-11 released on 2011-11-22 00:00:00." Database.

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Nonaka06: Nonaka G, Blankschien M, Herman C, Gross CA, Rhodius VA (2006). "Regulon and promoter analysis of the E. coli heat-shock factor, sigma32, reveals a multifaceted cellular response to heat stress." Genes Dev 20(13);1776-89. PMID: 16818608

Wade06: Wade JT, Roa DC, Grainger DC, Hurd D, Busby SJ, Struhl K, Nudler E (2006). "Extensive functional overlap between sigma factors in Escherichia coli." Nat Struct Mol Biol 13(9);806-14. PMID: 16892065


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Tue Nov 25, 2014, BIOCYC14B.