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Escherichia coli K-12 substr. MG1655 Polypeptide: glucose PTS permease - PtsG subunit



Gene: ptsG Accession Numbers: EG10787 (EcoCyc), b1101, ECK1087

Synonyms: umgC, umg, tgl, CR, car, cat, catB2, glcA, gptA, EIIBCglc, Enzyme IIBCglc, EIIBglc, Enzyme IIBglc

Regulation Summary Diagram: ?

Component of: glucose PTS permease (extended summary available)

Summary:
PtsG belongs to the bacterial phosphotransferase system and mediates uptake with concomittant phosphorylation of glucose. PtsG consists of a large hydrophobic domain (IIC domain) that spans the membrane 8 - 10 times and contains the sugar binding site plus a smaller hydrophilic domain (IIB domain) that is exposed on the cytoplasmic face of the membrane and is the site of phosphotransfer from EIIAglc (Crr) to O-6' of glucose. Purified PtsG is a homodimeric protein [Meins88, Buhr93, Zhuang99]. Phosphorylated PtsG is an intermediate during transport and phosphorylation of glucose [Begley82]. Purified EIIBCglc is phosphorylated in the presence of phosphoenolpyruvate, Enzyme I (PtsI), HPr (PtsH) and EIIAglc (Crr) [Meins93]. Cysteine residue 421 is required for transport and phosphorylation of glucose [Nuoffer88, Meins93].

PtsG may exist in two forms. It is oligomeric within the membrane and monomeric in its soluble form in the cytoplasm. Both forms exhibit PEP-dependent sugar phosphorylation and sugar phosphate-dependent transphosphorylation activity, though the kinetic properties of these reactions differ between the two forms of the protein [Aboulwafa03, Aboulwafa04, Aboulwafa11]. Soluble and membrane integrated forms of PtsG glc can be interconverted in vitro [Aboulwafa07].

The IIB domain of PtsG forms a spit α/β sandwich containing 4 β strands and 3 α helices. The active cysteine residue is located on a convex surface surrounded by hydrophobic residues [Eberstadt96, Golic94, Buhr94]. A mutant form of the IIC domain of PtsG has been overexpressed, purified and crystallised [Zurbriggen10, Jeckelmann11].

The monocistronic ptsG operon is subject to multiple layers of transcriptional and post-transcriptional control (reviewed in [Plumbridge02] and [Deutscher06]). Expression of ptsG is stimulated by the cyclic AMP-cyclic AMP receptor protein (CRP) complex and repressed by the Mlc transcriptional regulator [Kimata97, Plumbridge98, Kimata98, Zeppenfeld00, Lee00, Tanaka00].

Citations: [Erni86, Gutknecht98, Lanz98, Meadow05, Peterkofsky01]

Locations: cytosol, inner membrane

Map Position: [1,157,092 -> 1,158,525] (24.94 centisomes)
Length: 1434 bp / 477 aa

Molecular Weight of Polypeptide: 50.677 kD (from nucleotide sequence), 43.0 kD (experimental) [Bouma87 ]

Unification Links: ASAP:ABE-0003722 , CGSC:349 , DIP:DIP-29833N , EchoBASE:EB0780 , EcoGene:EG10787 , EcoliWiki:b1101 , ModBase:P69786 , OU-Microarray:b1101 , PortEco:ptsG , PR:PRO_000023631 , Pride:P69786 , Protein Model Portal:P69786 , RefSeq:NP_415619 , RegulonDB:EG10787 , SMR:P69786 , String:511145.b1101 , UniProt:P69786

Relationship Links: InterPro:IN-FAMILY:IPR001996 , InterPro:IN-FAMILY:IPR003352 , InterPro:IN-FAMILY:IPR004719 , InterPro:IN-FAMILY:IPR011299 , InterPro:IN-FAMILY:IPR011535 , InterPro:IN-FAMILY:IPR013013 , InterPro:IN-FAMILY:IPR018113 , PDB:Structure:1IBA , PDB:Structure:1O2F , PDB:Structure:3BP3 , PDB:Structure:3BP8 , Pfam:IN-FAMILY:PF00367 , Pfam:IN-FAMILY:PF02378 , Prosite:IN-FAMILY:PS01035 , Prosite:IN-FAMILY:PS51098 , Prosite:IN-FAMILY:PS51103

In Paralogous Gene Group: 176 (4 members) , 188 (6 members)

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from experiment Inferred by computational analysis [UniProtGOA11a, GOA01a, Bouma87, Meins93]
GO:0015758 - glucose transport Inferred from experiment Inferred by computational analysis [GOA01a, ValdiviaGonzale12]
GO:0034219 - carbohydrate transmembrane transport Inferred by computational analysis Inferred from experiment [Meins93, Nuoffer88, Bouma87, GOA01, GOA01a]
GO:0061490 - glucose import into cell Inferred from experiment [Bouma87, Curtis75, Stock82]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a]
GO:0008643 - carbohydrate transport Inferred by computational analysis [UniProtGOA11a]
GO:0016310 - phosphorylation Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0090564 - protein-phosphocysteine-glucose phosphotransferase system transporter activity Inferred from experiment [Bouma87, Nuoffer88, Meins93]
GO:0005355 - glucose transmembrane transporter activity Inferred by computational analysis [GOA01a]
GO:0008982 - protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred by computational analysis [GOA01, GOA01a]
GO:0016301 - kinase activity Inferred by computational analysis [UniProtGOA11a]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005829 - cytosol Inferred from experiment [Aboulwafa04]
GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Zhang07, Daley05]
GO:0005887 - integral component of plasma membrane Inferred from experiment [Meins88]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]

MultiFun Terms: cell structure membrane
information transfer protein related posttranslational modification
metabolism carbon utilization carbon compounds
regulation type of regulation posttranscriptional covalent modification, demodification, maturation
transport Group Translocators Phosphotransferase Systems (PEP-dependent PTS)

Essentiality data for ptsG knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of: glucose PTS permease

Synonyms: EIIglc, enzyme II glc

Subunit composition of glucose PTS permease = [PtsG]2[Crr]
         glucose PTS permease - PtsG subunit = PtsG (extended summary available)
         Enzyme IIAGlc; Crr = Crr (extended summary available)

Summary:
PtsG/Crr, the glucose-specific PTS permease, belongs to the functional superfamily of the phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system (PTSsugar). The PTSsugar transports and simultaneously phosphorylates its sugar substrates in a process called group translocation (reviewed in [Postma93]. PtsG/Crr takes up exogenous glucose, releasing the phosphate ester into the cell cytoplasm in preparation for metabolism, primarily via glycolysis. It also transports and phosphorylates the non-metabolizable analogue methyl α-D-glucoside [Curtis75, Stock82]. PtsG/Crr is a member of the PTS Glucose-Glucoside family of transporters [Saier14].

The glucose PTS permease, PtsG, possesses two domains in a single polypeptide chain with the domain order IIC-IIB and it functions with an additional polypeptide chain, the Crr or Enzyme IIAglc protein. The overall PTS-mediated phosphoryl transfer reaction, requiring the two general energy coupling proteins of the PTS, Enzyme I (PtsI) and HPr (PtsH), as well as the three domains of the Enzyme IIglc complex can be represented as:

PEP → Enzyme I-Phis189 → HPr-Phis15 → Enzyme IIA-Phis90 → Enzyme IIB-Pcys421 - (Enzyme IIC) → glucose-6-P

EIIglc activity is induced by growth in the presence of glucose [Kornberg72a, Erni86].

Citations: [GarciaAlles02, GarciaAlles02a, Pikis06]

GO Terms:

Biological Process: GO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from experiment [Bouma87, Meins93]
GO:0061490 - glucose import into cell Inferred from experiment [Curtis75, Bouma87]
Molecular Function: GO:0022855 - protein-N(PI)-phosphohistidine-glucose phosphotransferase system transporter activity Inferred from experiment [Bouma87, Meins93]
GO:0090564 - protein-phosphocysteine-glucose phosphotransferase system transporter activity Inferred from experiment [Nuoffer88, Meins93]

Credits:
Last-Curated ? 23-Mar-2014 by Mackie A , Macquarie University


Enzymatic reaction of: transport and phosphorylation of D-glucose (glucose PTS permease)

Synonyms: Transport of β-D-glucose


Enzymatic reaction of: transport and phosphorylation of methyl α-D-glucoside (glucose PTS permease)

Note: The enzyme may catalyze this reaction in vitro, but this reaction is not considered to be physiologically relevant.


Sequence Features

Feature Class Location Citations Comment
Conserved-Region 1 -> 388
[UniProt09]
UniProt: PTS EIIC type-1;
Transmembrane-Region 15 -> 35
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 51 -> 71
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 80 -> 100
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 112 -> 132
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 152 -> 172
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 191 -> 211
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 250 -> 270
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 280 -> 300
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 310 -> 330
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 356 -> 376
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Conserved-Region 399 -> 477
[UniProt09]
UniProt: PTS EIIB type-1;
Active-Site 421
[UniProt, 2010]
UniProt: Phosphocysteine intermediate; for EIIB activity;


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
10/20/97 Gene b1101 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10787; confirmed by SwissProt match.


References

Aboulwafa03: Aboulwafa M, Saier M (2003). "Soluble sugar permeases of the phosphotransferase system in Escherichia coli: evidence for two physically distinct forms of the proteins in vivo." Mol Microbiol 48(1);131-41. PMID: 12657050

Aboulwafa04: Aboulwafa M, Saier MH (2004). "Characterization of soluble enzyme II complexes of the Escherichia coli phosphotransferase system." J Bacteriol 186(24);8453-62. PMID: 15576795

Aboulwafa07: Aboulwafa M, Saier MH (2007). "In vitro interconversion of the soluble and membrane- integrated forms of the Escherichia coli glucose enzyme II of the phosphoenolpyruvate-dependent sugar-transporting phosphotransferase system." J Mol Microbiol Biotechnol 12(3-4);263-8. PMID: 17587874

Aboulwafa11: Aboulwafa M, Saier MH (2011). "Biophysical studies of the membrane-embedded and cytoplasmic forms of the glucose-specific Enzyme II of the E. coli phosphotransferase system (PTS)." PLoS One 6(9);e24088. PMID: 21935376

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Begley82: Begley GS, Hansen DE, Jacobson GR, Knowles JR (1982). "Stereochemical course of the reactions catalyzed by the bacterial phosphoenolpyruvate:glucose phosphotransferase system." Biochemistry 21 (22) 5552.

Bouma87: Bouma CL, Meadow ND, Stover EW, Roseman S (1987). "II-BGlc, a glucose receptor of the bacterial phosphotransferase system: molecular cloning of ptsG and purification of the receptor from an overproducing strain of Escherichia coli." Proc Natl Acad Sci U S A 84(4);930-4. PMID: 3029764

Buhr93: Buhr A, Erni B (1993). "Membrane topology of the glucose transporter of Escherichia coli." J Biol Chem 268(16);11599-603. PMID: 8505291

Buhr94: Buhr A, Flukiger K, Erni B (1994). "The glucose transporter of Escherichia coli. Overexpression, purification, and characterization of functional domains." J Biol Chem 269(38);23437-43. PMID: 8089109

Curtis75: Curtis SJ, Epstein W (1975). "Phosphorylation of D-glucose in Escherichia coli mutants defective in glucosephosphotransferase, mannosephosphotransferase, and glucokinase." J Bacteriol 122(3);1189-99. PMID: 1097393

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

Deutscher06: Deutscher J, Francke C, Postma PW (2006). "How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria." Microbiol Mol Biol Rev 70(4);939-1031. PMID: 17158705

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Eberstadt96: Eberstadt M, Grdadolnik SG, Gemmecker G, Kessler H, Buhr A, Erni B (1996). "Solution structure of the IIB domain of the glucose transporter of Escherichia coli." Biochemistry 35(35);11286-92. PMID: 8784182

Erni86: Erni B, Zanolari B (1986). "Glucose-permease of the bacterial phosphotransferase system. Gene cloning, overproduction, and amino acid sequence of enzyme IIGlc." J Biol Chem 261(35);16398-403. PMID: 3023349

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

GarciaAlles02: Garcia-Alles LF, Zahn A, Erni B (2002). "Sugar recognition by the glucose and mannose permeases of Escherichia coli. Steady-state kinetics and inhibition studies." Biochemistry 41(31);10077-86. PMID: 12146972

GarciaAlles02a: Garcia-Alles LF, Navdaeva V, Haenni S, Erni B (2002). "The glucose-specific carrier of the Escherichia coli phosphotransferase system." Eur J Biochem 269(20);4969-80. PMID: 12383255

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Golic94: Golic Grdadolnik S, Eberstadt M, Gemmecker G, Kessler H, Buhr A, Erni B (1994). "The glucose transporter of Escherichia coli. Assignment of the 1H, 13C and 15N resonances and identification of the secondary structure of the soluble IIB domain." Eur J Biochem 219(3);945-52. PMID: 8112346

Gutknecht98: Gutknecht R, Manni M, Mao Q, Erni B (1998). "The glucose transporter of Escherichia coli with circularly permuted domains is active in vivo and in vitro." J Biol Chem 273(40);25745-50. PMID: 9748244

Jeckelmann11: Jeckelmann JM, Harder D, Mari SA, Meury M, Ucurum Z, Muller DJ, Erni B, Fotiadis D (2011). "Structure and function of the glucose PTS transporter from Escherichia coli." J Struct Biol 176(3);395-403. PMID: 21996078

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Kimata97: Kimata K, Takahashi H, Inada T, Postma P, Aiba H (1997). "cAMP receptor protein-cAMP plays a crucial role in glucose-lactose diauxie by activating the major glucose transporter gene in Escherichia coli." Proc Natl Acad Sci U S A 94(24);12914-9. PMID: 9371775

Kimata98: Kimata K, Inada T, Tagami H, Aiba H (1998). "A global repressor (Mlc) is involved in glucose induction of the ptsG gene encoding major glucose transporter in Escherichia coli." Mol Microbiol 29(6);1509-19. PMID: 9781886

Kornberg72a: Kornberg HL, Reeves RE (1972). "Inducible phosphoenolpyruvate-dependent hexose phosphotransferase activities in Escherichia coli." Biochem J 128(5);1339-44. PMID: 4345358

Lanz98: Lanz R, Erni B (1998). "The glucose transporter of the Escherichia coli phosphotransferase system. Mutant analysis of the invariant arginines, histidines, and domain linker." J Biol Chem 273(20);12239-43. PMID: 9575173

Lee00: Lee SJ, Boos W, Bouche JP, Plumbridge J (2000). "Signal transduction between a membrane-bound transporter, PtsG, and a soluble transcription factor, Mlc, of Escherichia coli." EMBO J 19(20);5353-61. PMID: 11032803

Meadow05: Meadow ND, Savtchenko RS, Nezami A, Roseman S (2005). "Transient state kinetics of enzyme IICBGlc, a glucose transporter of the phosphoenolpyruvate phosphotransferase system of Escherichia coli: equilibrium and second order rate constants for the glucose binding and phosphotransfer reactions." J Biol Chem 280(51);41872-80. PMID: 16204242

Meins88: Meins M, Zanolari B, Rosenbusch JP, Erni B (1988). "Glucose permease of Escherichia coli. Purification of the IIGlc subunit and functional characterization of its oligomeric forms." J Biol Chem 263(26);12986-93. PMID: 3047116

Meins93: Meins M, Jeno P, Muller D, Richter WJ, Rosenbusch JP, Erni B (1993). "Cysteine phosphorylation of the glucose transporter of Escherichia coli." J Biol Chem 268(16);11604-9. PMID: 8505292

Nuoffer88: Nuoffer C, Zanolari B, Erni B (1988). "Glucose permease of Escherichia coli. The effect of cysteine to serine mutations on the function, stability, and regulation of transport and phosphorylation." J Biol Chem 263(14);6647-55. PMID: 3129430

Peterkofsky01: Peterkofsky A, Wang G, Garrett DS, Lee BR, Seok YJ, Clore GM (2001). "Three-dimensional structures of protein-protein complexes in the E. coli PTS." J Mol Microbiol Biotechnol 3(3);347-54. PMID: 11361064

Pikis06: Pikis A, Hess S, Arnold I, Erni B, Thompson J (2006). "Genetic requirements for growth of Escherichia coli K12 on methyl-alpha-D-glucopyranoside and the five alpha-D-glucosyl-D-fructose isomers of sucrose." J Biol Chem 281(26);17900-8. PMID: 16636060

Plumbridge02: Plumbridge J (2002). "Regulation of gene expression in the PTS in Escherichia coli: the role and interactions of Mlc." Curr Opin Microbiol 5(2);187-93. PMID: 11934616

Plumbridge98: Plumbridge J (1998). "Expression of ptsG, the gene for the major glucose PTS transporter in Escherichia coli, is repressed by Mlc and induced by growth on glucose." Mol Microbiol 1998;29(4);1053-63. PMID: 9767573

Postma93: Postma PW, Lengeler JW, Jacobson GR (1993). "Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria." Microbiol Rev 57(3);543-94. PMID: 8246840

Saier14: Saier MH, Reddy VS, Tamang DG, Vastermark A (2014). "The transporter classification database." Nucleic Acids Res 42(1);D251-8. PMID: 24225317

Stock82: Stock JB, Waygood EB, Meadow ND, Postma PW, Roseman S (1982). "Sugar transport by the bacterial phosphotransferase system. The glucose receptors of the Salmonella typhimurium phosphotransferase system." J Biol Chem 257(23);14543-52. PMID: 6292227

Tanaka00: Tanaka Y, Kimata K, Aiba H (2000). "A novel regulatory role of glucose transporter of Escherichia coli: membrane sequestration of a global repressor Mlc." EMBO J 19(20);5344-52. PMID: 11032802

UniProt, 2010: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

ValdiviaGonzale12: Valdivia-Gonzalez M, Perez-Donoso JM, Vasquez CC (2012). "Effect of tellurite-mediated oxidative stress on the Escherichia coli glycolytic pathway." Biometals 25(2);451-8. PMID: 22234496

Zeppenfeld00: Zeppenfeld T, Larisch C, Lengeler JW, Jahreis K (2000). "Glucose transporter mutants of Escherichia coli K-12 with changes in substrate recognition of IICB(Glc) and induction behavior of the ptsG gene." J Bacteriol 182(16);4443-52. PMID: 10913077

Zhang07: Zhang N, Chen R, Young N, Wishart D, Winter P, Weiner JH, Li L (2007). "Comparison of SDS- and methanol-assisted protein solubilization and digestion methods for Escherichia coli membrane proteome analysis by 2-D LC-MS/MS." Proteomics 7(4);484-93. PMID: 17309111

Zhuang99: Zhuang J, Gutknecht R, Flukiger K, Hasler L, Erni B, Engel A (1999). "Purification and electron microscopic characterization of the membrane subunit (IICB(Glc)) of the Escherichia coli glucose transporter." Arch Biochem Biophys 372(1);89-96. PMID: 10562420

Zurbriggen10: Zurbriggen A, Schneider P, Bahler P, Baumann U, Erni B (2010). "Expression, purification, crystallization and preliminary X-ray analysis of the EIICGlc domain of the Escherichia coli glucose transporter." Acta Crystallogr Sect F Struct Biol Cryst Commun 66(Pt 6);684-8. PMID: 20516600

Other References Related to Gene Regulation

Chung13: Chung D, Park D, Myers K, Grass J, Kiley P, Landick R, Keles S (2013). "dPeak: high resolution identification of transcription factor binding sites from PET and SET ChIP-Seq data." PLoS Comput Biol 9(10);e1003246. PMID: 24146601

Gohler12: Gohler AK, Staab A, Gabor E, Homann K, Klang E, Kosfeld A, Muus JE, Wulftange JS, Jahreis K (2012). "Characterization of MtfA, a novel regulatory output signal protein of the glucose-phosphotransferase system in Escherichia coli K-12." J Bacteriol 194(5);1024-35. PMID: 22178967

Jeong04: Jeong JY, Kim YJ, Cho N, Shin D, Nam TW, Ryu S, Seok YJ (2004). "Expression of ptsG Encoding the Major Glucose Transporter Is Regulated by ArcA in Escherichia coli." J Biol Chem 279(37);38513-8. PMID: 15252051

Kawamoto06: Kawamoto H, Koide Y, Morita T, Aiba H (2006). "Base-pairing requirement for RNA silencing by a bacterial small RNA and acceleration of duplex formation by Hfq." Mol Microbiol 61(4);1013-22. PMID: 16859494

Kimata01: Kimata K, Tanaka Y, Inada T, Aiba H (2001). "Expression of the glucose transporter gene, ptsG, is regulated at the mRNA degradation step in response to glycolytic flux in Escherichia coli." EMBO J 20(13);3587-95. PMID: 11432845

Kumar11: Kumar R, Shimizu K (2011). "Transcriptional regulation of main metabolic pathways of cyoA, cydB, fnr, and fur gene knockout Escherichia coli in C-limited and N-limited aerobic continuous cultures." Microb Cell Fact 10;3. PMID: 21272324

Marzan13: Marzan LW, Hasan CM, Shimizu K (2013). "Effect of acidic condition on the metabolic regulation of Escherichia coli and its phoB mutant." Arch Microbiol 195(3);161-71. PMID: 23274360

Pennetier10: Pennetier C, Oberto J, Plumbridge J (2010). "An antisense transcript from within the ptsG promoter region in Escherichia coli." J Mol Microbiol Biotechnol 18(4);230-40. PMID: 20668389

Plumbridge01: Plumbridge J (2001). "DNA binding sites for the Mlc and NagC proteins: regulation of nagE, encoding the N-acetylglucosamine-specific transporter in Escherichia coli." Nucleic Acids Res 29(2);506-14. PMID: 11139621

Rungrassamee08: Rungrassamee W, Liu X, Pomposiello PJ (2008). "Activation of glucose transport under oxidative stress in Escherichia coli." Arch Microbiol 190(1):41-9. PMID: 18368388

ShalelLevanon05: Shalel-Levanon S, San KY, Bennett GN (2005). "Effect of ArcA and FNR on the expression of genes related to the oxygen regulation and the glycolysis pathway in Escherichia coli under microaerobic growth conditions." Biotechnol Bioeng 92(2):147-59. PMID: 15988767

Shin01a: Shin D, Lim S, Seok YJ, Ryu S (2001). "Heat shock RNA polymerase (E sigma(32)) is involved in the transcription of mlc and crucial for induction of the Mlc regulon by glucose in Escherichia coli." J Biol Chem 276(28);25871-5. PMID: 11340070

Shin03: Shin D, Cho N, Heu S, Ryu S (2003). "Selective regulation of ptsG expression by Fis. Formation of either activating or repressing nucleoprotein complex in response to glucose." J Biol Chem 278(17);14776-81. PMID: 12588863

Shin08a: Shin D, Cho N, Kim YJ, Seok YJ, Ryu S (2008). "Up-regulation of the cellular level of Escherichia coli PTS components by stabilizing reduced transcripts of the genes in response to the low oxygen level." Biochem Biophys Res Commun 370(4);609-12. PMID: 18402772

Vanderpool04: Vanderpool CK, Gottesman S (2004). "Involvement of a novel transcriptional activator and small RNA in post-transcriptional regulation of the glucose phosphoenolpyruvate phosphotransferase system." Mol Microbiol 54(4);1076-89. PMID: 15522088

Zheng04: Zheng D, Constantinidou C, Hobman JL, Minchin SD (2004). "Identification of the CRP regulon using in vitro and in vivo transcriptional profiling." Nucleic Acids Res 32(19);5874-93. PMID: 15520470


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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