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Escherichia coli K-12 substr. MG1655 Enzyme: pyruvate:ferredoxin oxidoreductase

Summary:
The main activity of this enzyme appears to be the reduction of ferredoxin using the oxidation of pyruvate as the electron source. Ferredoxin can be replaced by flavodoxin and a variety of other electron acceptors [Blaschkowski82].

The molecular weight of this enzyme is approximately 200 kDa by gel filtration [Blaschkowski82]. According to [Kalim09], it is most likely identical to YdbK, pyruvate:flavodoxin oxidoreductase.

Molecular Weight of Polypeptide: 200.0 kD (experimental) [Blaschkowski82 ]

Gene-Reaction Schematic: ?

Credits:
Last-Curated ? 30-Jul-2009 by Keseler I , SRI International


Enzymatic reaction of: pyruvate:ferredoxin oxidoreductase

Synonyms: pyruvate-flavodoxin reductase, pyruvate:ferredoxin 2-oxidoreductase (CoA-acetylating), pyruvate-dependent reductase, CoA-acylating pyruvate:flavodoxin oxidoreductase, pyruvate synthase

EC Number: 1.2.7.1

pyruvate + 2 an oxidized ferredoxin + coenzyme A <=> acetyl-CoA + CO2 + 2 a reduced ferredoxin + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is physiologically favored in the direction shown.

Alternative Substrates for an oxidized ferredoxin: FAD [Blaschkowski82 ] , FMN [Blaschkowski82 ] , an oxidized flavodoxin [Blaschkowski82 ]

Cofactors or Prosthetic Groups: thiamin diphosphate [Blaschkowski82]


References

Blaschkowski82: Blaschkowski HP, Neuer G, Ludwig-Festl M, Knappe J (1982). "Routes of flavodoxin and ferredoxin reduction in Escherichia coli. CoA-acylating pyruvate: flavodoxin and NADPH: flavodoxin oxidoreductases participating in the activation of pyruvate formate-lyase." Eur J Biochem 1982;123(3);563-9. PMID: 7042345

Kalim09: Kalim Akhtar M, Jones PR (2009). "Construction of a synthetic YdbK-dependent pyruvate:H(2) pathway in Escherichia coli BL21(DE3)." Metab Eng 11(3);139-47. PMID: 19558967


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sat Dec 20, 2014, BIOCYC14B.