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Escherichia coli K-12 substr. MG1655 Transporter: serine:H+ symporter SdaC



Gene: sdaC Accession Numbers: EG12142 (EcoCyc), b2796, ECK2791

Synonyms: dcrA, SdaC serine STP transporter

Regulation Summary Diagram: ?

Summary:
SdaC is a probable serine uptake system, likely to function as a serine/proton symporter. Expression of the cloned sdaC resulted in an increased rate of serine uptake and this uptake was insensitive to threonine [Shao94]. SdaC likely corresponds to the threonine-insensitive serine/proton symport system described in a nhaB mutant [Kayahara92]. This sytem was suggested to be a high affinity serine-specific transporter with a Km of 5-6 μM. SdaC is a member of the STP family of amino acid transporters, and is homologous to the threonine/serine transporter TdcC.

The sdaC is part of an operon which includes the sdaB gene, encoding a serine deaminase [Shao94]. SdaB has been suggested to have a degradative, energy-providing role, implying that serine imported via SdaC is used primarily for energy production. Resistance of an sdaC mutant to colicin V has revealed that SdaC is an inner membrane receptor for this peptide antibiotic [Gerard05].

Citations: [Likhacheva96, Samsonov02]

Locations: inner membrane

Map Position: [2,926,251 -> 2,927,540] (63.07 centisomes)
Length: 1290 bp / 429 aa

Molecular Weight of Polypeptide: 46.906 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0009169 , CGSC:33329 , EchoBASE:EB2063 , EcoGene:EG12142 , EcoliWiki:b2796 , OU-Microarray:b2796 , PortEco:sdaC , PR:PRO_000023917 , Pride:P0AAD6 , Protein Model Portal:P0AAD6 , RefSeq:NP_417276 , RegulonDB:EG12142 , SMR:P0AAD6 , String:511145.b2796 , UniProt:P0AAD6

Relationship Links: InterPro:IN-FAMILY:IPR004694

In Paralogous Gene Group: 458 (6 members)

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11, GOA01]
Molecular Function: GO:0005215 - transporter activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, DiazMejia09, Daley05]
GO:0005887 - integral component of plasma membrane Inferred from experiment [Hsieh10]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11, GOA01]

MultiFun Terms: cell structure membrane
metabolism biosynthesis of building blocks amino acids serine
transport Electrochemical potential driven transporters Porters (Uni-, Sym- and Antiporters)

Essentiality data for sdaC knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Enzymatic reaction of: serine:H+ symporter SdaC

Synonyms: Transport of L-serine


Sequence Features

Feature Class Location Citations Comment
Transmembrane-Region 23 -> 43
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 47 -> 67
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 100 -> 120
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 141 -> 161
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 164 -> 184
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 202 -> 222
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 250 -> 270
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 298 -> 318
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 348 -> 368
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 370 -> 390
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 407 -> 427
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b2796 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12142; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerard05: Gerard F, Pradel N, Wu LF (2005). "Bactericidal activity of colicin V is mediated by an inner membrane protein, SdaC, of Escherichia coli." J Bacteriol 187(6);1945-50. PMID: 15743941

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Hsieh10: Hsieh JM, Besserer GM, Madej MG, Bui HQ, Kwon S, Abramson J (2010). "Bridging the gap: a GFP-based strategy for overexpression and purification of membrane proteins with intra and extracellular C-termini." Protein Sci 19(4);868-80. PMID: 20196076

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Kayahara92: Kayahara T, Thelen P, Ogawa W, Inaba K, Tsuda M, Goldberg EB, Tsuchiya T (1992). "Properties of recombinant cells capable of growing on serine without NhaB Na+/H+ antiporter in Escherichia coli." J Bacteriol 1992;174(22);7482-5. PMID: 1331028

Likhacheva96: Likhacheva NA, Samsonov VV, Samsonov VV, Sineoky SP (1996). "Genetic control of the resistance to phage C1 of Escherichia coli K-12." J Bacteriol 178(17);5309-15. PMID: 8752353

Samsonov02: Samsonov VV, Samsonov VV, Sineoky SP (2002). "DcrA and dcrB Escherichia coli genes can control DNA injection by phages specific for BtuB and FhuA receptors." Res Microbiol 153(10);639-46. PMID: 12558182

Shao94: Shao Z, Lin RT, Newman EB (1994). "Sequencing and characterization of the sdaC gene and identification of the sdaCB operon in Escherichia coli K12." Eur J Biochem 1994;222(3);901-7. PMID: 8026499

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Wed Nov 26, 2014, biocyc13.