Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
twitter

Escherichia coli K-12 substr. MG1655 Polypeptide: predicted PTS permease - SgcA subunit



Gene: sgcA Accession Numbers: EG12554 (EcoCyc), b4302, ECK4291

Synonyms: yjhL, EIIASgc, Enzyme IIASgc

Regulation Summary Diagram: ?

Component of: predicted PTS permease SgcBCA (extended summary available)

Summary:
sgcA contains an Enzyme IIA domain [Reizer96b].

Locations: cytosol

Map Position: [4,525,572 <- 4,526,003] (97.54 centisomes)
Length: 432 bp / 143 aa

Molecular Weight of Polypeptide: 15.638 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0014100 , EchoBASE:EB2442 , EcoGene:EG12554 , EcoliWiki:b4302 , ModBase:P39363 , OU-Microarray:b4302 , PortEco:sgcA , Protein Model Portal:P39363 , RefSeq:NP_418722 , RegulonDB:EG12554 , SMR:P39363 , String:511145.b4302 , UniProt:P39363

Relationship Links: InterPro:IN-FAMILY:IPR002178 , InterPro:IN-FAMILY:IPR016152 , Pfam:IN-FAMILY:PF00359 , Prosite:IN-FAMILY:PS00372 , Prosite:IN-FAMILY:PS51094

In Paralogous Gene Group: 187 (14 members)

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0008643 - carbohydrate transport Inferred by computational analysis [UniProtGOA11]
GO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred by computational analysis [UniProtGOA11, Reizer96b]
GO:0016310 - phosphorylation Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0005215 - transporter activity Inferred by computational analysis [GOA01]
GO:0016301 - kinase activity Inferred by computational analysis [UniProtGOA11]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11]
Cellular Component: GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11a, UniProtGOA11]
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: transport Group Translocators Phosphotransferase Systems (PEP-dependent PTS)

Essentiality data for sgcA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Subunit of: predicted PTS permease SgcBCA

Synonyms: EIISgc, Enzyme IISgc

Subunit composition of predicted PTS permease SgcBCA = [SgcB][SgcC][SgcA]
         predicted PTS permease - SgcB subunit = SgcB (summary available)
         predicted PTS permease - SgcC subunit = SgcC (summary available)
         predicted PTS permease - SgcA subunit = SgcA (summary available)

Summary:
SgcBCA, a putative PTS permease, belongs to the functional superfamily of the phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system (PTSsugar). The PTSsugar transports and simultaneously phosphorylates its substrates in a process called group translocation (reviewed in [Postma93].

sgcA is homologous to the IIA domains of the fructose- and mannitol-specific PTS permeases. sgcA has the highest similarity to IIA domains of the L-ascorbate PTS and the cryptic mannitol PTS. sgcC has 40% identity with the IIC domain of the galactitol PTS. sgcC encodes a protein with 9 or 10 predicted transmembrane regions [Reizer96b]. sgcB shows sequence similarity to the IIB domain of the galactitol-specific PTS permease [Reizer97]. The function of these proteins is not known [Reizer96b].

Please note that the expression of the sgc gene cluster (sgcREAQCBX) has not been studied and the presence of an sgcBCA encoded protein complex is hypothetical.

GO Terms:

Biological Process: GO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred by computational analysis [Reizer96b, Reizer97]

Credits:
Revised 29-Apr-2014 by Mackie A , Macquarie University
Last-Curated ? 29-Apr-2014 by Mackie A , Macquarie University


Sequence Features

Feature Class Location Citations Comment
Conserved-Region 1 -> 143
[UniProt09]
UniProt: PTS EIIA type-2;
Active-Site 63
[UniProt10]
UniProt: Tele-phosphohistidine intermediate; Non-Experimental Qualifier: by similarity;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b4302 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12554; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Postma93: Postma PW, Lengeler JW, Jacobson GR (1993). "Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria." Microbiol Rev 57(3);543-94. PMID: 8246840

Reizer96b: Reizer J, Charbit A, Reizer A, Saier MH (1996). "Novel Phosphotransferase System Genes Revealed by Bacterial Genome Analysis: Operons Encoding Homologues of Sugar-Specific Permease Domains of the Phosphotransferase System and Pentose Catabolic Enzymes." Genome Science and Technology, 1(2), 53-75.

Reizer97: Reizer J, Reizer A, Saier MH (1997). "Is the ribulose monophosphate pathway widely distributed in bacteria?." Microbiology 143 ( Pt 8);2519-20. PMID: 9274005

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Thu Nov 27, 2014, BIOCYC14B.