Escherichia coli K-12 substr. MG1655 Polypeptide: predicted PTS permease - SgcA subunit

Gene: sgcA Accession Numbers: EG12554 (EcoCyc), b4302, ECK4291

Synonyms: yjhL, EIIASgc, Enzyme IIASgc

Regulation Summary Diagram: ?

Regulation summary diagram for sgcA

Component of: predicted PTS permease SgcBCA (extended summary available)

sgcA contains an Enzyme IIA domain [Reizer96b].

Locations: cytosol

Map Position: [4,525,572 <- 4,526,003] (97.54 centisomes, 351°)
Length: 432 bp / 143 aa

Molecular Weight of Polypeptide: 15.638 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0014100 , EchoBASE:EB2442 , EcoGene:EG12554 , EcoliWiki:b4302 , ModBase:P39363 , OU-Microarray:b4302 , PortEco:sgcA , Protein Model Portal:P39363 , RefSeq:NP_418722 , RegulonDB:EG12554 , SMR:P39363 , String:511145.b4302 , UniProt:P39363

Relationship Links: InterPro:IN-FAMILY:IPR002178 , InterPro:IN-FAMILY:IPR016152 , Pfam:IN-FAMILY:PF00359 , Prosite:IN-FAMILY:PS00372 , Prosite:IN-FAMILY:PS51094

In Paralogous Gene Group: 187 (14 members)

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0008643 - carbohydrate transport Inferred by computational analysis [UniProtGOA11]
GO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred by computational analysis [UniProtGOA11, Reizer96b]
GO:0016310 - phosphorylation Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0005215 - transporter activity Inferred by computational analysis [GOA01]
GO:0016301 - kinase activity Inferred by computational analysis [UniProtGOA11]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11]
Cellular Component: GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11a, UniProtGOA11]
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: transport Group Translocators Phosphotransferase Systems (PEP-dependent PTS)

Essentiality data for sgcA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Subunit of: predicted PTS permease SgcBCA

Synonyms: EIISgc, Enzyme IISgc

Subunit composition of predicted PTS permease SgcBCA = [SgcB][SgcC][SgcA]
         predicted PTS permease - SgcB subunit = SgcB (summary available)
         predicted PTS permease - SgcC subunit = SgcC (summary available)
         predicted PTS permease - SgcA subunit = SgcA (summary available)

SgcBCA, a putative PTS permease, belongs to the functional superfamily of the phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system (PTSsugar). The PTSsugar transports and simultaneously phosphorylates its substrates in a process called group translocation (reviewed in [Postma93].

sgcA is homologous to the IIA domains of the fructose- and mannitol-specific PTS permeases. sgcA has the highest similarity to IIA domains of the L-ascorbate PTS and the cryptic mannitol PTS. sgcC has 40% identity with the IIC domain of the galactitol PTS. sgcC encodes a protein with 9 or 10 predicted transmembrane regions [Reizer96b]. sgcB shows sequence similarity to the IIB domain of the galactitol-specific PTS permease [Reizer97a]. The function of these proteins is not known [Reizer96b].

Please note that the expression of the sgc gene cluster (sgcREAQCBX) has not been studied and the presence of an sgcBCA encoded protein complex is hypothetical.

GO Terms:

Biological Process: GO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred by computational analysis [Reizer96b, Reizer97a]

Revised 29-Apr-2014 by Mackie A , Macquarie University
Last-Curated ? 29-Apr-2014 by Mackie A , Macquarie University

Sequence Features

Protein sequence of predicted PTS permease - SgcA subunit with features indicated

Feature Class Location Citations Comment
Conserved-Region 1 -> 143
UniProt: PTS EIIA type-2;
Active-Site 63
UniProt: Tele-phosphohistidine intermediate; Non-Experimental Qualifier: by similarity;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


10/20/97 Gene b4302 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12554; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Postma93: Postma PW, Lengeler JW, Jacobson GR (1993). "Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria." Microbiol Rev 57(3);543-94. PMID: 8246840

Reizer96b: Reizer J, Charbit A, Reizer A, Saier MH (1996). "Novel Phosphotransferase System Genes Revealed by Bacterial Genome Analysis: Operons Encoding Homologues of Sugar-Specific Permease Domains of the Phosphotransferase System and Pentose Catabolic Enzymes." Genome Science and Technology, 1(2), 53-75.

Reizer97a: Reizer J, Reizer A, Saier MH (1997). "Is the ribulose monophosphate pathway widely distributed in bacteria?." Microbiology 143 ( Pt 8);2519-20. PMID: 9274005

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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