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Escherichia coli K-12 substr. MG1655 Polypeptide: predicted PTS permease - SgcC subunit



Gene: sgcC Accession Numbers: EG12556 (EcoCyc), b4304, ECK4293

Synonyms: yjhN, EIICSgc, Enzyme IICSgc

Regulation Summary Diagram: ?

Component of: predicted PTS permease SgcBCA (extended summary available)

Summary:
sgcC is predicted to encode a protein with 9 or 10 transmembrane helices; it shows sequence similarity to the Enzyme IIC domain of the galactitol PTS [Reizer96].

Locations: inner membrane

Map Position: [4,526,953 <- 4,528,266] (97.57 centisomes)
Length: 1314 bp / 437 aa

Molecular Weight of Polypeptide: 46.684 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0014105 , EchoBASE:EB2444 , EcoGene:EG12556 , EcoliWiki:b4304 , OU-Microarray:b4304 , PortEco:sgcC , Pride:P39365 , Protein Model Portal:P39365 , RefSeq:NP_418724 , RegulonDB:EG12556 , String:511145.b4304 , UniProt:P39365

Relationship Links: EcoO157Cyc:Homolog:Z4877 , EcoO157Cyc:Homolog:Z4877-MONOMER , InterPro:IN-FAMILY:IPR004703 , InterPro:IN-FAMILY:IPR013014 , InterPro:IN-FAMILY:IPR013853 , Pfam:IN-FAMILY:PF03611 , Prosite:IN-FAMILY:PS51104

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
GO:0008643 - carbohydrate transport Inferred by computational analysis [UniProtGOA11]
GO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred by computational analysis [UniProtGOA11, GOA01, Reizer96]
GO:0015796 - galactitol transport Inferred by computational analysis [GOA01]
Molecular Function: GO:0015577 - galactitol transmembrane transporter activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, DiazMejia09, Daley05]
GO:0005887 - integral component of plasma membrane Inferred by computational analysis [Reizer96]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11, GOA01]

MultiFun Terms: transport Group Translocators Phosphotransferase Systems (PEP-dependent PTS)

Essentiality data for sgcC knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Subunit of: predicted PTS permease SgcBCA

Synonyms: EIISgc, Enzyme IISgc

Subunit composition of predicted PTS permease SgcBCA = [SgcB][SgcC][SgcA]
         predicted PTS permease - SgcB subunit = SgcB (summary available)
         predicted PTS permease - SgcC subunit = SgcC (summary available)
         predicted PTS permease - SgcA subunit = SgcA (summary available)

Summary:
SgcBCA, a putative PTS permease, belongs to the functional superfamily of the phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system (PTSsugar). The PTSsugar transports and simultaneously phosphorylates its substrates in a process called group translocation (reviewed in [Postma93].

sgcA is homologous to the IIA domains of the fructose- and mannitol-specific PTS permeases. sgcA has the highest similarity to IIA domains of the L-ascorbate PTS and the cryptic mannitol PTS. sgcC has 40% identity with the IIC domain of the galactitol PTS. sgcC encodes a protein with 9 or 10 predicted transmembrane regions [Reizer96]. sgcB shows sequence similarity to the IIB domain of the galactitol-specific PTS permease [Reizer97]. The function of these proteins is not known [Reizer96].

Please note that the expression of the sgc gene cluster (sgcREAQCBX) has not been studied and the presence of an sgcBCA encoded protein complex is hypothetical.

GO Terms:

Biological Process: GO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred by computational analysis [Reizer96, Reizer97]

Credits:
Revised 29-Apr-2014 by Mackie A , Macquarie University
Last-Curated ? 29-Apr-2014 by Mackie A , Macquarie University


Sequence Features

Feature Class Location Citations Comment
Conserved-Region 2 -> 437
[UniProt09]
UniProt: PTS EIIC type-2;
Transmembrane-Region 5 -> 25
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 35 -> 55
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 88 -> 108
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 134 -> 154
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 173 -> 193
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 215 -> 235
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 236 -> 256
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 302 -> 322
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 325 -> 345
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 354 -> 374
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 385 -> 405
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 410 -> 430
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b4304 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12556; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Postma93: Postma PW, Lengeler JW, Jacobson GR (1993). "Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria." Microbiol Rev 57(3);543-94. PMID: 8246840

Reizer96: Reizer J, Charbit A, Reizer A, Saier MH (1996). "Novel Phosphotransferase System Genes Revealed by Bacterial Genome Analysis: Operons Encoding Homologues of Sugar-Specific Permease Domains of the Phosphotransferase System and Pentose Catabolic Enzymes." Genome Science and Technology, 1(2), 53-75.

Reizer97: Reizer J, Reizer A, Saier MH (1997). "Is the ribulose monophosphate pathway widely distributed in bacteria?." Microbiology 143 ( Pt 8);2519-20. PMID: 9274005

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Mon Nov 24, 2014, biocyc13.