Escherichia coli K-12 substr. MG1655 Transporter: shikimate:H+ symporter

Gene: shiA Accession Numbers: G7067 (EcoCyc), b1981, ECK1976

Synonyms: yeeM, ShiA shikimate MFS transporter

Regulation Summary Diagram: ?

Regulation summary diagram for shiA

ShiA has been implicated in the high affinity transport of shikimate, an intermediate in the aromatic amino acid biosynthetic pathway [Whipp98, Brown76]. Chromosomal mutants in shiA are unable to transport shikimate, and introduction of the cloned shiA gene restores shikimate transport [Whipp98]. The ShiA protein has twelve predicted TMS and is a member of the major facilitator superfamily of transporters (MFS) [Pao98] and hence is likely to function as a proton/shikimate symporter. Analysis of a shiA-lacZ fusion has suggested that shiA expression is constitutive and is not regulated by the TyrR repressor [Whipp98]. The shiA gene probably constitutes a monocistronic operon. Imported shikimate presumably serves as a substrate for biosynthesis of aromatic compounds.

Locations: inner membrane

Map Position: [2,051,667 -> 2,052,983] (44.22 centisomes, 159°)
Length: 1317 bp / 438 aa

Molecular Weight of Polypeptide: 47.817 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0006572 , EchoBASE:EB4148 , EcoGene:EG20205 , EcoliWiki:b1981 , ModBase:P76350 , OU-Microarray:b1981 , PortEco:shiA , PR:PRO_000023947 , Pride:P76350 , Protein Model Portal:P76350 , RefSeq:NP_416488 , RegulonDB:G7067 , SMR:P76350 , String:511145.b1981 , UniProt:P76350

Relationship Links: InterPro:IN-FAMILY:IPR004736 , InterPro:IN-FAMILY:IPR005828 , InterPro:IN-FAMILY:IPR005829 , InterPro:IN-FAMILY:IPR020846 , Pfam:IN-FAMILY:PF00083 , Prosite:IN-FAMILY:PS00217 , Prosite:IN-FAMILY:PS50850

In Paralogous Gene Group: 332 (5 members)

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Genetic Regulation Schematic: ?

Genetic regulation schematic for shiA

GO Terms:

Biological Process: GO:0015733 - shikimate transport Inferred from experiment [Whipp98]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11, GOA01a]
GO:0055085 - transmembrane transport Inferred by computational analysis [GOA01a]
Molecular Function: GO:0015530 - shikimate transmembrane transporter activity Inferred from experiment [Whipp98]
GO:0005215 - transporter activity Inferred by computational analysis [GOA01a]
GO:0015293 - symporter activity Inferred by computational analysis [UniProtGOA11]
GO:0022857 - transmembrane transporter activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, DiazMejia09, Daley05]
GO:0005887 - integral component of plasma membrane Inferred by computational analysis [Gaudet10]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11, GOA01a]

MultiFun Terms: cell structure membrane
metabolism biosynthesis of building blocks amino acids chorismate
transport Electrochemical potential driven transporters Porters (Uni-, Sym- and Antiporters)

Essentiality data for shiA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Enzymatic reaction of: shikimate:H+ symporter

Synonyms: Transport of shikimate

Transport reaction diagram for shikimate:H+ symporter

Sequence Features

Protein sequence of shikimate:H+ symporter with features indicated

Feature Class Location Citations Comment
Transmembrane-Region 28 -> 48
UniProt: Helical; Name=1;; Non-Experimental Qualifier: potential;
Transmembrane-Region 64 -> 84
UniProt: Helical; Name=2;; Non-Experimental Qualifier: potential;
Transmembrane-Region 109 -> 129
UniProt: Helical; Name=3;; Non-Experimental Qualifier: potential;
Transmembrane-Region 133 -> 153
UniProt: Helical; Name=4;; Non-Experimental Qualifier: potential;
Transmembrane-Region 168 -> 188
UniProt: Helical; Name=5;; Non-Experimental Qualifier: potential;
Transmembrane-Region 193 -> 213
UniProt: Helical; Name=6;; Non-Experimental Qualifier: potential;
Transmembrane-Region 255 -> 275
UniProt: Helical; Name=7;; Non-Experimental Qualifier: potential;
Transmembrane-Region 287 -> 307
UniProt: Helical; Name=8;; Non-Experimental Qualifier: potential;
Transmembrane-Region 318 -> 337
UniProt: Helical; Name=9;; Non-Experimental Qualifier: potential;
Transmembrane-Region 341 -> 363
UniProt: Helical; Name=10;; Non-Experimental Qualifier: potential;
Transmembrane-Region 387 -> 407
UniProt: Helical; Name=11;; Non-Experimental Qualifier: potential;
Transmembrane-Region 411 -> 431
UniProt: Helical; Name=12;; Non-Experimental Qualifier: potential;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


3/2/1998 (pkarp) Merged genes G446/EG20205 and G7067/shiA
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Brown76: Brown KD, Doy CH (1976). "Transport and utilization of the biosynthetic intermediate shikimic acid in Escherichia coli." Biochim Biophys Acta 1976;428(3);550-62. PMID: 6050

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gaudet10: Gaudet P, Livstone M, Thomas P (2010). "Annotation inferences using phylogenetic trees." PMID: 19578431

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Pao98: Pao SS, Paulsen IT, Saier MH (1998). "Major facilitator superfamily." Microbiol Mol Biol Rev 1998;62(1);1-34. PMID: 9529885

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Whipp98: Whipp MJ, Camakaris H, Pittard AJ (1998). "Cloning and analysis of the shiA gene, which encodes the shikimate transport system of Escherichia coli K-12." Gene 1998;209(1-2);185-92. PMID: 9524262

Other References Related to Gene Regulation

Prevost07: Prevost K, Salvail H, Desnoyers G, Jacques JF, Phaneuf E, Masse E (2007). "The small RNA RyhB activates the translation of shiA mRNA encoding a permease of shikimate, a compound involved in siderophore synthesis." Mol Microbiol 64(5);1260-73. PMID: 17542919

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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