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Escherichia coli K-12 substr. MG1655 Enzyme: dTDP-fucosamine acetyltransferase



Gene: rffC Accession Numbers: EG11455 (EcoCyc), b3790, ECK3782

Synonyms: rff, wecD, yifH

Regulation Summary Diagram: ?

Summary:
dTDP-fucosamine acetyltransferase catalyzes the synthesis of TDP-4-acetamido-4,6-dideoxy-D-galactose, which is utilized in the biosynthesis of Enterobacterial Common Antigen (ECA). Surprisingly little experimental work on this enzyme has been published.

Crystal structures of RffC from the uropathogenic strain CFT073, both in the apo form and in complex with acetyl-CoA, have been solved at 1.95 and 1.66 Å resolution, respectively [Hung06].

Mutants with a defective rffC gene accumulate lipid III [MeierDieter90, MeierDieter92].

Locations: inner membrane

Map Position: [3,972,619 -> 3,973,164] (85.62 centisomes)
Length: 546 bp / 181 aa

Molecular Weight of Polypeptide: 19.545 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0012382 , EchoBASE:EB1424 , EcoGene:EG11455 , EcoliWiki:b3790 , ModBase:P27832 , OU-Microarray:b3790 , PortEco:rffC , Protein Model Portal:P27832 , RefSeq:YP_026256 , RegulonDB:EG11455 , SMR:P27832 , String:511145.b3790 , UniProt:P27832

Relationship Links: InterPro:IN-FAMILY:IPR000182 , InterPro:IN-FAMILY:IPR002110 , InterPro:IN-FAMILY:IPR012752 , InterPro:IN-FAMILY:IPR016181 , InterPro:IN-FAMILY:IPR020683 , Pfam:IN-FAMILY:PF00583 , Prosite:IN-FAMILY:PS51186

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0009246 - enterobacterial common antigen biosynthetic process Inferred from experiment Inferred by computational analysis [GOA01, Rahman01]
GO:0009103 - lipopolysaccharide biosynthetic process Inferred by computational analysis [UniProtGOA11]
GO:0009243 - O antigen biosynthetic process Inferred by computational analysis [UniProtGOA12]
Molecular Function: GO:0008080 - N-acetyltransferase activity Inferred from experiment Inferred by computational analysis [GOA01, Matsuhashi64]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11]
GO:0016746 - transferase activity, transferring acyl groups Inferred by computational analysis [UniProtGOA11]
Cellular Component: GO:0005886 - plasma membrane

MultiFun Terms: cell structure surface antigens (ECA, O antigen of LPS)

Essentiality data for rffC knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Credits:
Curated 02-Aug-2006 by Keseler I , SRI International
Last-Curated ? 03-Jul-2013 by Kubo A , SRI International


Enzymatic reaction of: dTDP-fucosamine acetyltransferase

EC Number: 2.3.1.210

dTDP-thomosamine + acetyl-CoA <=> dTDP-N-acetylthomosamine + coenzyme A + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the Enzyme Commission system.

The reaction is favored in the direction shown.

In Pathways: enterobacterial common antigen biosynthesis , dTDP-N-acetylthomosamine biosynthesis


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b3790 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11455; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Hung06: Hung MN, Rangarajan E, Munger C, Nadeau G, Sulea T, Matte A (2006). "Crystal structure of TDP-fucosamine acetyltransferase (WecD) from Escherichia coli, an enzyme required for enterobacterial common antigen synthesis." J Bacteriol 188(15);5606-17. PMID: 16855251

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Matsuhashi64: Matsuhashi M, Strominger JL (1964). "Thymidine Diphosphate 4-Acetamido-4, 6-dideoxyhexoses. I. Enzymatic Synthesis by Strains of Escherichia coli." J Biol Chem 239;2454-63. PMID: 14235522

MeierDieter90: Meier-Dieter U, Starman R, Barr K, Mayer H, Rick PD (1990). "Biosynthesis of enterobacterial common antigen in Escherichia coli. Biochemical characterization of Tn10 insertion mutants defective in enterobacterial common antigen synthesis." J Biol Chem 1990;265(23);13490-7. PMID: 2166030

MeierDieter92: Meier-Dieter U, Barr K, Starman R, Hatch L, Rick PD (1992). "Nucleotide sequence of the Escherichia coli rfe gene involved in the synthesis of enterobacterial common antigen. Molecular cloning of the rfe-rff gene cluster." J Biol Chem 1992;267(2);746-53. PMID: 1730666

Rahman01: Rahman A, Barr K, Rick PD (2001). "Identification of the structural gene for the TDP-Fuc4NAc:lipid II Fuc4NAc transferase involved in synthesis of enterobacterial common antigen in Escherichia coli K-12." J Bacteriol 183(22);6509-16. PMID: 11673418

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA12: UniProt-GOA (2012). "Gene Ontology annotation based on UniPathway vocabulary mapping."

Other References Related to Gene Regulation

Huerta03: Huerta AM, Collado-Vides J (2003). "Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals." J Mol Biol 333(2);261-78. PMID: 14529615

Partridge09: Partridge JD, Bodenmiller DM, Humphrys MS, Spiro S (2009). "NsrR targets in the Escherichia coli genome: new insights into DNA sequence requirements for binding and a role for NsrR in the regulation of motility." Mol Microbiol 73(4);680-94. PMID: 19656291


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sun Nov 23, 2014, biocyc14.