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Escherichia coli K-12 substr. MG1655 Transporter: K+ transporter TrkG



Gene: trkG Accession Numbers: EG11020 (EcoCyc), b1363, ECK1362

Regulation Summary Diagram: ?

Summary:
TrkG is a potassium ion transporter, closely related to the TrkH potassium ion transporter. Inactivation of either the trkG or trkH gene affects the kinetics of potassium uptake [Schlosser95]. Kinetic studies suggest that TrkG and TrkH are both active as low affinity high rate potassium ion transporters (Km of 1-6 mM) [Schlosser95] and function in conjunction with TrkA, a peripheral membrane binding protein that binds NAD+ and is essential for TrkG/H activity [Bossemeyer89, Schlosser93]. TrkE, an ATP binding protein, is required for TrkH activity and affects the kinetics of TrkG activity [Dosch91, Harms01]. TrkG and TrkH are members of the Trk family of potassium ion transporters and probably function as potassium ion/proton symporters.

TrkG is an inner membrane protein with 10 - 12 predicted transmembrane regions [Schlosser91]. Similar to TrkH, TrkG is predicted to contain 4 sequential MPM (membrane-pore-membrane) regions plus two additional TM regions [Durell99].

trkG displays atypical codon usage and has a G+C content of only 37%. The trkG gene is probably a foreign gene which was inserted with a rac prophage [Schlosser91].

Trk mediated K+ transport in E. coli requires both ATP and the proton motive force [Rhoads77] however Trk systems are not energised directly by ATP [Stewart85].

Citations: [Epstein71, CorratgeFaillie10]

Locations: inner membrane

Map Position: [1,421,806 -> 1,423,263] (30.64 centisomes)
Length: 1458 bp / 485 aa

Molecular Weight of Polypeptide: 53.944 kD (from nucleotide sequence), 35.0 kD (experimental) [Schlosser91 ]

Unification Links: ASAP:ABE-0004567 , CGSC:29963 , DIP:DIP-11029N , EchoBASE:EB1013 , EcoGene:EG11020 , EcoliWiki:b1363 , OU-Microarray:b1363 , PortEco:trkG , PR:PRO_000024113 , Protein Model Portal:P23849 , RefSeq:NP_415881 , RegulonDB:EG11020 , SMR:P23849 , String:511145.b1363 , UniProt:P23849

Relationship Links: InterPro:IN-FAMILY:IPR003445 , InterPro:IN-FAMILY:IPR004772 , Pfam:IN-FAMILY:PF02386

In Paralogous Gene Group: 282 (2 members)

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0006813 - potassium ion transport Inferred from experiment Inferred by computational analysis [UniProtGOA11, Schlosser91]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
GO:0006811 - ion transport Inferred by computational analysis [UniProtGOA11]
GO:0006812 - cation transport Inferred by computational analysis [GOA01]
GO:0055085 - transmembrane transport Inferred by computational analysis [GOA01]
GO:0071805 - potassium ion transmembrane transport Inferred by computational analysis [GOA01, GOA]
Molecular Function: GO:0015388 - potassium uptake transmembrane transporter activity Inferred from experiment [Dosch91, Schlosser91]
GO:0005267 - potassium channel activity Inferred by computational analysis [GOA]
GO:0008324 - cation transmembrane transporter activity Inferred by computational analysis [GOA01]
GO:0022820 - potassium ion symporter activity Inferred by computational analysis [GOA01]
GO:0030955 - potassium ion binding Inferred by computational analysis [GOA]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, DiazMejia09, Daley05]
GO:0005887 - integral component of plasma membrane Inferred from experiment [Schlosser91]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11, GOA01]

MultiFun Terms: cell structure membrane
extrachromosomal prophage genes and phage related functions
transport Electrochemical potential driven transporters Porters (Uni-, Sym- and Antiporters)

Essentiality data for trkG knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Credits:
Last-Curated ? 11-Feb-2014 by Mackie A , Macquarie University


Enzymatic reaction of: K+ transporter TrkG

Alternative Products for K+: Rb+ [Schlosser95 ]


Sequence Features

Feature Class Location Citations Comment
Transmembrane-Region 6 -> 32
[UniProt12a]
UniProt: Helical; Non-Experimental Qualifier: by similarity.
Transmembrane-Region 39 -> 60
[UniProt12a]
UniProt: Helical; Non-Experimental Qualifier: by similarity.
Transmembrane-Region 69 -> 93
[UniProt12a]
UniProt: Helical; Non-Experimental Qualifier: by similarity.
Intramembrane-Region 101 -> 112
[UniProt12a]
UniProt: Helical; Pore-forming;; Non-Experimental Qualifier: by similarity.
Intramembrane-Region 113 -> 118
[UniProt12a]
UniProt: Non-Experimental Qualifier: by similarity.
Amino-Acid-Sites-That-Bind 114
[UniProt12a]
UniProt: Potassium ion; via carbonyl oxygen; Non-Experimental Qualifier: by similarity.
Amino-Acid-Sites-That-Bind 115
[UniProt12a]
UniProt: Potassium ion; via carbonyl oxygen; Non-Experimental Qualifier: by similarity.
Transmembrane-Region 128 -> 153
[UniProt12a]
UniProt: Helical; Non-Experimental Qualifier: by similarity.
Transmembrane-Region 181 -> 205
[UniProt12a]
UniProt: Helical; Non-Experimental Qualifier: by similarity.
Intramembrane-Region 209
[UniProt12a]
UniProt: Non-Experimental Qualifier: by similarity.
Intramembrane-Region 210 -> 221
[UniProt12a]
UniProt: Helical; Pore-forming;; Non-Experimental Qualifier: by similarity.
Intramembrane-Region 222 -> 227
[UniProt12a]
UniProt: Non-Experimental Qualifier: by similarity.
Amino-Acid-Sites-That-Bind 224
[UniProt12a]
UniProt: Potassium ion; via carbonyl oxygen; Non-Experimental Qualifier: by similarity.
Intramembrane-Region 238 -> 253
[UniProt12a]
UniProt: Helical; Non-Experimental Qualifier: by similarity.
Transmembrane-Region 277 -> 297
[UniProt12a]
UniProt: Helical; Non-Experimental Qualifier: by similarity.
Intramembrane-Region 303 -> 318
[UniProt12a]
UniProt: Helical; Pore-forming;; Non-Experimental Qualifier: by similarity.
Intramembrane-Region 319 -> 324
[UniProt12a]
UniProt: Non-Experimental Qualifier: by similarity.
Intramembrane-Region 333 -> 344
[UniProt12a]
UniProt: Helical; Non-Experimental Qualifier: by similarity.
Intramembrane-Region 345 -> 357
[UniProt12a]
UniProt: Note=Loop between two helices; Non-Experimental Qualifier: by similarity.
Transmembrane-Region 392 -> 419
[UniProt12a]
UniProt: Helical; Non-Experimental Qualifier: by similarity.
Intramembrane-Region 422 -> 423
[UniProt12a]
UniProt: Non-Experimental Qualifier: by similarity.
Intramembrane-Region 424 -> 434
[UniProt12a]
UniProt: Helical; Pore-forming;; Non-Experimental Qualifier: by similarity.
Intramembrane-Region 435 -> 441
[UniProt12a]
UniProt: Non-Experimental Qualifier: by similarity.
Protein-Segment 436 -> 441
[UniProt12a]
UniProt: Selectivity filter part 4; Sequence Annotation Type: region of interest; Non-Experimental Qualifier: by similarity.
Amino-Acid-Sites-That-Bind 437
[UniProt12a]
UniProt: Potassium ion; via carbonyl oxygen; Non-Experimental Qualifier: by similarity.
Intramembrane-Region 454 -> 465
[UniProt12a]
UniProt: Helical; Non-Experimental Qualifier: by similarity.


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b1363 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11020; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Bossemeyer89: Bossemeyer D, Borchard A, Dosch DC, Helmer GC, Epstein W, Booth IR, Bakker EP (1989). "K+-transport protein TrkA of Escherichia coli is a peripheral membrane protein that requires other trk gene products for attachment to the cytoplasmic membrane." J Biol Chem 1989;264(28);16403-10. PMID: 2674131

CorratgeFaillie10: Corratge-Faillie C, Jabnoune M, Zimmermann S, Very AA, Fizames C, Sentenac H (2010). "Potassium and sodium transport in non-animal cells: the Trk/Ktr/HKT transporter family." Cell Mol Life Sci 67(15);2511-32. PMID: 20333436

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Dosch91: Dosch DC, Helmer GL, Sutton SH, Salvacion FF, Epstein W (1991). "Genetic analysis of potassium transport loci in Escherichia coli: evidence for three constitutive systems mediating uptake potassium." J Bacteriol 1991;173(2);687-96. PMID: 1987159

Durell99: Durell SR, Hao Y, Nakamura T, Bakker EP, Guy HR (1999). "Evolutionary relationship between K(+) channels and symporters." Biophys J 77(2);775-88. PMID: 10423425

Epstein71: Epstein W, Kim BS (1971). "Potassium transport loci in Escherichia coli K-12." J Bacteriol 108(2);639-44. PMID: 4942756

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA: GOA "Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity."

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Harms01: Harms C, Domoto Y, Celik C, Rahe E, Stumpe S, Schmid R, Nakamura T, Bakker EP (2001). "Identification of the ABC protein SapD as the subunit that confers ATP dependence to the K+-uptake systems Trk(H) and Trk(G) from Escherichia coli K-12." Microbiology 2001;147(Pt 11);2991-3003. PMID: 11700350

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Rhoads77: Rhoads DB, Epstein W (1977). "Energy coupling to net K+ transport in Escherichia coli K-12." J Biol Chem 252(4);1394-401. PMID: 320207

Schlosser91: Schlosser A, Kluttig S, Hamann A, Bakker EP (1991). "Subcloning, nucleotide sequence, and expression of trkG, a gene that encodes an integral membrane protein involved in potassium uptake via the Trk system of Escherichia coli." J Bacteriol 173(10);3170-6. PMID: 2022616

Schlosser93: Schlosser A, Hamann A, Bossemeyer D, Schneider E, Bakker EP (1993). "NAD+ binding to the Escherichia coli K(+)-uptake protein TrkA and sequence similarity between TrkA and domains of a family of dehydrogenases suggest a role for NAD+ in bacterial transport." Mol Microbiol 1993;9(3);533-43. PMID: 8412700

Schlosser95: Schlosser A, Meldorf M, Stumpe S, Bakker EP, Epstein W (1995). "TrkH and its homolog, TrkG, determine the specificity and kinetics of cation transport by the Trk system of Escherichia coli." J Bacteriol 1995;177(7);1908-10. PMID: 7896723

Stewart85: Stewart LM, Bakker EP, Booth IR (1985). "Energy coupling to K+ uptake via the Trk system in Escherichia coli: the role of ATP." J Gen Microbiol 131(1);77-85. PMID: 3886836

UniProt12a: UniProt Consortium (2012). "UniProt version 2012-11 released on 2012-11-26 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sun Dec 21, 2014, BIOCYC13B.