Escherichia coli K-12 substr. MG1655 Polypeptide: putative transport protein, ABC superfamily - ATP-binding component

Gene: yadG Accession Numbers: EG12320 (EcoCyc), b0127, ECK0126

Regulation Summary Diagram: ?

Regulation summary diagram for yadG

Component of: putative transporter complex, ABC superfamily (summary available)

YadG is an ATP-binding component of a predicted ABC superfamily efflux transporter.

yadG transcription is increased specifically in response to 2,4,6-trinitrotoluene and its indicator compounds 1,3-DNB, 2,4-DNT, and 2,6-DNT; the response was utilized for construction of an E. coli biosensor strain [Tan15].

Locations: inner membrane

Map Position: [142,779 -> 143,705] (3.08 centisomes, 11°)
Length: 927 bp / 308 aa

Molecular Weight of Polypeptide: 34.647 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0000447 , DIP:DIP-11185N , EchoBASE:EB2225 , EcoGene:EG12320 , EcoliWiki:b0127 , Mint:MINT-1289416 , ModBase:P36879 , OU-Microarray:b0127 , PortEco:yadG , Pride:P36879 , Protein Model Portal:P36879 , RefSeq:NP_414669 , RegulonDB:EG12320 , SMR:P36879 , String:511145.b0127 , UniProt:P36879

Relationship Links: InterPro:IN-FAMILY:IPR003439 , InterPro:IN-FAMILY:IPR003593 , InterPro:IN-FAMILY:IPR017871 , InterPro:IN-FAMILY:IPR027417 , Pfam:IN-FAMILY:PF00005 , Prosite:IN-FAMILY:PS00211 , Prosite:IN-FAMILY:PS50893 , Smart:IN-FAMILY:SM00382

In Paralogous Gene Group: 23 (75 members)

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a]
GO:0008152 - metabolic process Inferred by computational analysis [GOA01a]
Molecular Function: GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11a]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0016887 - ATPase activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [DiazMejia09, LopezCampistrou05]

MultiFun Terms: transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily ATP binding cytoplasmic component

Essentiality data for yadG knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Last-Curated ? 12-Jan-2015 by Keseler I , SRI International

Subunit of: putative transporter complex, ABC superfamily

Subunit composition of putative transporter complex, ABC superfamily = [YadG][YadH]
         putative transport protein, ABC superfamily - ATP-binding component = YadG (summary available)
         putative transport protein, ABC superfamily - membrane component = YadH (summary available)

YadG and YadH are uncharacterized members of the ABC superfamily of transporters [Saurin99]. YadG is the putative ATP binding component, YadH is the putative membrane compenent. Based on sequence similarity, they probably function together as as an ATP-driven efflux system. The genes yadG and yadH are probably located within a single operon.

Sequence Features

Protein sequence of putative transport protein, ABC superfamily - ATP-binding component with features indicated

Feature Class Location Citations Comment
Conserved-Region 5 -> 236
UniProt: ABC transporter;
Nucleotide-Phosphate-Binding-Region 38 -> 45
UniProt: ATP; Non-Experimental Qualifier: potential;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


10/20/97 Gene b0127 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12320; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

LopezCampistrou05: Lopez-Campistrous A, Semchuk P, Burke L, Palmer-Stone T, Brokx SJ, Broderick G, Bottorff D, Bolch S, Weiner JH, Ellison MJ (2005). "Localization, annotation, and comparison of the Escherichia coli K-12 proteome under two states of growth." Mol Cell Proteomics 4(8);1205-9. PMID: 15911532

Saurin99: Saurin W, Hofnung M, Dassa E (1999). "Getting in or out: early segregation between importers and exporters in the evolution of ATP-binding cassette (ABC) transporters." J Mol Evol 1999;48(1);22-41. PMID: 9873074

Tan15: Tan J, Kan N, Wang W, Ling J, Qu G, Jin J, Shao Y, Liu G, Chen H (2015). "Construction of 2,4,6-Trinitrotoluene Biosensors with Novel Sensing Elements from Escherichia coli K-12 MG1655." Cell Biochem Biophys. PMID: 25561288

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Fri Oct 9, 2015, biocyc12.