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Escherichia coli K-12 substr. MG1655 Polypeptide: YbbA



Gene: ybbA Accession Numbers: EG11657 (EcoCyc), b0495, ECK0489

Regulation Summary Diagram: ?

Component of: YbbA/YbbP ABC transporter (summary available)

Summary:
YbbA is an ATP-binding component of a predicted ABC superfamily metabolite uptake transporter.

Locations: inner membrane, cytosol

Map Position: [518,957 -> 519,643] (11.19 centisomes)
Length: 687 bp / 228 aa

Molecular Weight of Polypeptide: 24.533 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0001717 , EchoBASE:EB1609 , EcoGene:EG11657 , EcoliWiki:b0495 , Mint:MINT-1283721 , ModBase:P0A9T8 , OU-Microarray:b0495 , PortEco:ybbA , Pride:P0A9T8 , Protein Model Portal:P0A9T8 , RefSeq:NP_415028 , RegulonDB:EG11657 , SMR:P0A9T8 , String:511145.b0495 , UniProt:P0A9T8

Relationship Links: InterPro:IN-FAMILY:IPR003439 , InterPro:IN-FAMILY:IPR003593 , InterPro:IN-FAMILY:IPR017871 , InterPro:IN-FAMILY:IPR027417 , Pfam:IN-FAMILY:PF00005 , Prosite:IN-FAMILY:PS00211 , Prosite:IN-FAMILY:PS50893 , Smart:IN-FAMILY:SM00382

In Paralogous Gene Group: 23 (75 members)

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0006200 - ATP catabolic process Inferred by computational analysis [GOA01]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0016887 - ATPase activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0005886 - plasma membrane Inferred by computational analysis [DiazMejia09]

MultiFun Terms: transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily ATP binding cytoplasmic component

Essentiality data for ybbA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Subunit of: YbbA/YbbP ABC transporter

Subunit composition of YbbA/YbbP ABC transporter = [YbbP]2[YbbA]2

Summary:
YbbA and YbbP are uncharacterized members of the ABC superfamily of transporters [Saurin99]. YbbA is the putative ATP binding component. YbbP may be the membrane component. Based on sequence similarity, these proteins may work together as part of an ATP dependent metabolite uptake system. The genes ybbA, and ybbP are probably located within a single operon.


Sequence Features

Feature Class Location Citations Comment
Conserved-Region 7 -> 228
[UniProt09]
UniProt: ABC transporter;
Nucleotide-Phosphate-Binding-Region 43 -> 50
[UniProt10]
UniProt: ATP; Non-Experimental Qualifier: potential;
Sequence-Conflict 88 -> 89
[Cho93, UniProt10a]
Alternate sequence: KH → ND; UniProt: (in Ref. 4; L06182);


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b0495 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11657; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Cho93: Cho H, Cronan JE (1993). "Escherichia coli thioesterase I, molecular cloning and sequencing of the structural gene and identification as a periplasmic enzyme." J Biol Chem 1993;268(13);9238-45. PMID: 8098033

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Saurin99: Saurin W, Hofnung M, Dassa E (1999). "Getting in or out: early segregation between importers and exporters in the evolution of ATP-binding cassette (ABC) transporters." J Mol Evol 1999;48(1);22-41. PMID: 9873074

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 28, 2014, biocyc14.