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Escherichia coli K-12 substr. MG1655 Transporter: enterobactin efflux transporter EntS



Gene: entS Accession Numbers: EG11104 (EcoCyc), b0591, ECK0584

Synonyms: ybdA

Regulation Summary Diagram: ?

Component of: EntS-TolC Enterobactin Efflux Transport System (summary available)

Summary:
entS was identified in a screen for genes that reduce the lethal effects of stress. An entS insertion mutant is more sensitive than wild type to mitomycin C and other stresses [Han10b]. EntS has been implicated in arabinose efflux [Koita12].

Locations: inner membrane

Map Position: [621,523 -> 622,773] (13.4 centisomes)
Length: 1251 bp / 416 aa

Molecular Weight of Polypeptide: 43.282 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0002036 , EchoBASE:EB1096 , EcoGene:EG11104 , EcoliWiki:b0591 , ModBase:P24077 , OU-Microarray:b0591 , PortEco:entS , PR:PRO_000022525 , Protein Model Portal:P24077 , RefSeq:NP_415123 , RegulonDB:EG11104 , SMR:P24077 , String:511145.b0591 , UniProt:P24077

Relationship Links: InterPro:IN-FAMILY:IPR010290 , InterPro:IN-FAMILY:IPR016196 , InterPro:IN-FAMILY:IPR020846 , InterPro:IN-FAMILY:IPR023722 , Pfam:IN-FAMILY:PF05977 , Prosite:IN-FAMILY:PS50850

In Paralogous Gene Group: 159 (2 members)

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0006974 - cellular response to DNA damage stimulus Inferred from experiment [Han10b]
GO:0042930 - enterobactin transport Inferred from experiment Inferred by computational analysis [GOA01, Bleuel05]
GO:0046677 - response to antibiotic Inferred from experiment [Han10b]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
GO:0015891 - siderophore transport Inferred by computational analysis [GOA06]
Molecular Function: GO:0042931 - enterobactin transporter activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, GOA06, GOA01, DiazMejia09, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11]

MultiFun Terms: cell structure membrane
transport Electrochemical potential driven transporters Porters (Uni-, Sym- and Antiporters)

Essentiality data for entS knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]
Yes [Feist07, Comment 2]

Enzymatic reaction of: enterobactin efflux transporter EntS


Subunit of: EntS-TolC Enterobactin Efflux Transport System

Subunit composition of EntS-TolC Enterobactin Efflux Transport System = [EntS][(TolC)3]
         enterobactin efflux transporter EntS = EntS (summary available)
         TolC outer membrane channel = (TolC)3 (extended summary available)
                 TolC monomer = TolC

Summary:
The EntS protein is a member of the major facilitator superfamily (MFS) of transporters [Pao98]. Based on sequence similarity, it functions as a proton-driven efflux system. Siderophore nutrition assays have shown that an entS mutant is unable to export enterobactin efficiently to alleviate iron deprivation [Furrer02], though some export does still occur through another mechanism. Deletion of tolC abolishes enterobactin export completely. Enterobactin appears to have more than one mechanism for export to the periplasm, one involving EntS, but TolC is the only outer membrane channel that can export enterobactin from the periplasm [Bleuel05].


Enzymatic reaction of: EntS-TolC Enterobactin Efflux Transport System


Sequence Features

Feature Class Location Citations Comment
Transmembrane-Region 22 -> 42
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 56 -> 76
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 96 -> 116
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 119 -> 139
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Sequence-Conflict 123
[Shea91, UniProt10]
Alternate sequence: F → S; UniProt: (in Ref. 1; CAA40706);
Transmembrane-Region 157 -> 177
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 179 -> 199
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 219 -> 239
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 257 -> 277
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 301 -> 321
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 357 -> 377
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 379 -> 399
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
10/20/97 Gene b0591 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11104; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Bleuel05: Bleuel C, Grosse C, Taudte N, Scherer J, Wesenberg D, Krauss GJ, Nies DH, Grass G (2005). "TolC is involved in enterobactin efflux across the outer membrane of Escherichia coli." J Bacteriol 187(19);6701-7. PMID: 16166532

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Furrer02: Furrer JL, Sanders DN, Hook-Barnard IG, McIntosh MA (2002). "Export of the siderophore enterobactin in Escherichia coli: involvement of a 43 kDa membrane exporter." Mol Microbiol 44(5);1225-34. PMID: 12068807

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Han10b: Han X, Dorsey-Oresto A, Malik M, Wang JY, Drlica K, Zhao X, Lu T (2010). "Escherichia coli genes that reduce the lethal effects of stress." BMC Microbiol 10;35. PMID: 20128927

Koita12: Koita K, Rao CV (2012). "Identification and Analysis of the Putative Pentose Sugar Efflux Transporters in Escherichia coli." PLoS One 7(8);e43700. PMID: 22952739

Pao98: Pao SS, Paulsen IT, Saier MH (1998). "Major facilitator superfamily." Microbiol Mol Biol Rev 1998;62(1);1-34. PMID: 9529885

Shea91: Shea CM, McIntosh MA (1991). "Nucleotide sequence and genetic organization of the ferric enterobactin transport system: homology to other periplasmic binding protein-dependent systems in Escherichia coli." Mol Microbiol 1991;5(6);1415-28. PMID: 1838574

UniProt10: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Chen07: Chen Z, Lewis KA, Shultzaberger RK, Lyakhov IG, Zheng M, Doan B, Storz G, Schneider TD (2007). "Discovery of Fur binding site clusters in Escherichia coli by information theory models." Nucleic Acids Res 35(20);6762-77. PMID: 17921503

Chenault91: Chenault SS, Earhart CF (1991). "Organization of genes encoding membrane proteins of the Escherichia coli ferrienterobactin permease." Mol Microbiol 1991;5(6);1405-13. PMID: 1787794

Christoffersen01: Christoffersen CA, Brickman TJ, Hook-Barnard I, McIntosh MA (2001). "Regulatory architecture of the iron-regulated fepD-ybdA bidirectional promoter region in Escherichia coli." J Bacteriol 183(6);2059-70. PMID: 11222606

Lavrrar02: Lavrrar JL, Christoffersen CA, McIntosh MA (2002). "Fur-DNA interactions at the bidirectional fepDGC-entS promoter region in Escherichia coli." J Mol Biol 322(5);983-95. PMID: 12367523


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Mon Dec 22, 2014, biocyc13.