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Escherichia coli K-12 substr. MG1655 Polypeptide: predicted sugar transporter subunit: ATP-binding component of ABC superfamily



Gene: ycjV Accession Numbers: G6656 (EcoCyc), b1318, ECK1313

Regulation Summary Diagram: ?

Summary:
YcjV is an uncharacterized member of the ABC superfamily of transporters [Saurin99]. It is a putative ATP-binding protein. Based on sequence similarity it may function as part of an ATP-dependent sugar transporter.

Locations: inner membrane, cytosol

Map Position: [1,378,845 -> 1,379,813] (29.72 centisomes)
Length: 969 bp / 322 aa

Molecular Weight of Polypeptide: 35.767 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0004422 , DIP:DIP-11613N , EchoBASE:EB3678 , EcoGene:EG13919 , EcoliWiki:b1318 , ModBase:P77481 , OU-Microarray:b1318 , PortEco:ycjV , Pride:P77481 , Protein Model Portal:P77481 , RegulonDB:G6656 , SMR:P77481 , Swiss-Model:P77481 , UniProt:P77481

Relationship Links: InterPro:IN-FAMILY:IPR003439 , InterPro:IN-FAMILY:IPR003593 , InterPro:IN-FAMILY:IPR008995 , InterPro:IN-FAMILY:IPR012340 , InterPro:IN-FAMILY:IPR013611 , InterPro:IN-FAMILY:IPR017871 , InterPro:IN-FAMILY:IPR027417 , Pfam:IN-FAMILY:PF00005 , Pfam:IN-FAMILY:PF08402 , Prosite:IN-FAMILY:PS00211 , Prosite:IN-FAMILY:PS50893 , Smart:IN-FAMILY:SM00382

In Paralogous Gene Group: 23 (75 members)

GO Terms:

Biological Process: GO:0006200 - ATP catabolic process Inferred by computational analysis [GOA et al., 2001]
GO:0006810 - transport Inferred by computational analysis [UniProt-GOA, 2011]
GO:0055085 - transmembrane transport Inferred by computational analysis [GOA et al., 2001]
Molecular Function: GO:0000166 - nucleotide binding Inferred by computational analysis [UniProt-GOA, 2011]
GO:0005524 - ATP binding Inferred by computational analysis [UniProt-GOA, 2011, GOA et al., 2001]
GO:0016820 - hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances Inferred by computational analysis [GOA et al., 2001]
GO:0016887 - ATPase activity Inferred by computational analysis [GOA et al., 2001]
Cellular Component: GO:0005829 - cytosol
GO:0043190 - ATP-binding cassette (ABC) transporter complex Inferred by computational analysis [GOA et al., 2001]

MultiFun Terms: transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily ATP binding cytoplasmic component

Essentiality data for ycjV knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes et al., 2003, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba et al., 2006, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce et al., 2006, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba et al., 2006, Comment 2]

Sequence Features

Feature Class Location Citations Comment
Conserved-Region 4 -> 235
[UniProt, 2009]
UniProt: ABC transporter;
Sequence-Conflict 37 -> 38
[Fajardo98, UniProt, 2010]
Alternate sequence: GP → AA; UniProt: (in Ref. 1; AAC34719);
Nucleotide-Phosphate-Binding-Region 37 -> 44
[UniProt10]
UniProt: ATP; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba et al., 2006: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Fajardo98: Fajardo DA, Cheung J, Ito C, Sugawara E, Nikaido H, Misra R (1998). "Biochemistry and regulation of a novel Escherichia coli K-12 porin protein, OmpG, which produces unusually large channels." J Bacteriol 1998;180(17);4452-9. PMID: 9721282

Gerdes et al., 2003: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA et al., 2001: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce et al., 2006: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Saurin99: Saurin W, Hofnung M, Dassa E (1999). "Getting in or out: early segregation between importers and exporters in the evolution of ATP-binding cassette (ABC) transporters." J Mol Evol 1999;48(1);22-41. PMID: 9873074

UniProt, 2009: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt, 2010: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt-GOA, 2011: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Thu Dec 18, 2014, biocyc11.