Escherichia coli K-12 substr. MG1655 Polypeptide: conserved inner membrane protein

Gene: yjcH Accession Numbers: EG11943 (EcoCyc), b4068, ECK4061

Regulation Summary Diagram: ?

Regulation summary diagram for yjcH

yjcH is cotranscribed with the acs and actP genes, which encode proteins involved in acetate dissimilation and transport [Gimenez03].

Gene Citations: [Kumari00]

Locations: inner membrane

Map Position: [4,282,922 <- 4,283,236] (92.31 centisomes, 332°)
Length: 315 bp / 104 aa

Molecular Weight of Polypeptide: 11.672 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0013330 , EchoBASE:EB1887 , EcoGene:EG11943 , EcoliWiki:b4068 , OU-Microarray:b4068 , PortEco:yjcH , Protein Model Portal:P0AF54 , RefSeq:NP_418492 , RegulonDB:EG11943 , String:511145.b4068 , UniProt:P0AF54

Relationship Links: InterPro:IN-FAMILY:IPR007436 , Pfam:IN-FAMILY:PF04341

Genetic Regulation Schematic: ?

Genetic regulation schematic for yjcH

GO Terms:

Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11]

Gene Class: ORFs Conserved-ORFs

Essentiality data for yjcH knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Last-Curated ? 07-Jun-2006 by Keseler I , SRI International

Sequence Features

Protein sequence of conserved inner membrane protein with features indicated

Feature Class Location Citations Comment
Transmembrane-Region 25 -> 47
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 62 -> 84
UniProt: Helical;; Non-Experimental Qualifier: potential;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Units:

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram


Peter D. Karp on Thu Jan 16, 2003:
Predicted gene function revised as a result of E. coli genome reannotation by Serres et al. [Serres01 ].
10/20/97 Gene b4068 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11943; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

Gimenez03: Gimenez R, Nunez MF, Badia J, Aguilar J, Baldoma L (2003). "The gene yjcG, cotranscribed with the gene acs, encodes an acetate permease in Escherichia coli." J Bacteriol 185(21);6448-55. PMID: 14563880

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Kumari00: Kumari S, Beatty CM, Browning DF, Busby SJ, Simel EJ, Hovel-Miner G, Wolfe AJ (2000). "Regulation of acetyl coenzyme A synthetase in Escherichia coli." J Bacteriol 182(15);4173-9. PMID: 10894724

Serres01: Serres MH, Gopal S, Nahum LA, Liang P, Gaasterland T, Riley M (2001). "A functional update of the Escherichia coli K-12 genome." Genome Biol 2(9);RESEARCH0035. PMID: 11574054

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Beatty03: Beatty CM, Browning DF, Busby SJ, Wolfe AJ (2003). "Cyclic AMP receptor protein-dependent activation of the Escherichia coli acsP2 promoter by a synergistic class III mechanism." J Bacteriol 185(17);5148-57. PMID: 12923087

Browning02: Browning DF, Beatty CM, Wolfe AJ, Cole JA, Busby SJ (2002). "Independent regulation of the divergent Escherichia coli nrfA and acsP1 promoters by a nucleoprotein assembly at a shared regulatory region." Mol Microbiol 43(3);687-701. PMID: 11929525

Browning04a: Browning DF, Beatty CM, Sanstad EA, Gunn KE, Busby SJ, Wolfe AJ (2004). "Modulation of CRP-dependent transcription at the Escherichia coli acsP2 promoter by nucleoprotein complexes: anti-activation by the nucleoid proteins FIS and IHF." Mol Microbiol 51(1);241-54. PMID: 14651625

Browning05: Browning DF, Grainger DC, Beatty CM, Wolfe AJ, Cole JA, Busby SJ (2005). "Integration of three signals at the Escherichia coli nrf promoter: a role for Fis protein in catabolite repression." Mol Microbiol 57(2);496-510. PMID: 15978080

Browning06: Browning DF, Lee DJ, Wolfe AJ, Cole JA, Busby SJ (2006). "The Escherichia coli K-12 NarL and NarP proteins insulate the nrf promoter from the effects of integration host factor." J Bacteriol 188(21);7449-56. PMID: 16936015

Kumari00a: Kumari S, Simel EJ, Wolfe AJ (2000). "sigma(70) is the principal sigma factor responsible for transcription of acs, which encodes acetyl coenzyme A synthetase in Escherichia coli." J Bacteriol 182(2);551-4. PMID: 10629211

Olvera09: Olvera L, Mendoza-Vargas A, Flores N, Olvera M, Sigala JC, Gosset G, Morett E, Bolivar F (2009). "Transcription analysis of central metabolism genes in Escherichia coli. Possible roles of sigma38 in their expression, as a response to carbon limitation." PLoS One 4(10);e7466. PMID: 19838295

Sclavi07: Sclavi B, Beatty CM, Thach DS, Fredericks CE, Buckle M, Wolfe AJ (2007). "The multiple roles of CRP at the complex acs promoter depend on activation region 2 and IHF." Mol Microbiol 65(2);425-40. PMID: 17630973

Shin97a: Shin S, Song SG, Lee DS, Pan JG, Park C (1997). "Involvement of iclR and rpoS in the induction of acs, the gene for acetyl coenzyme A synthetase of Escherichia coli K-12." FEMS Microbiol Lett 1997;146(1);103-8. PMID: 8997713

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Wed Oct 7, 2015, biocyc13.