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Escherichia coli K-12 substr. MG1655 Polypeptide: D-allose ABC transporter - ATP binding subunit



Gene: alsA Accession Numbers: EG11959 (EcoCyc), b4087, ECK4080

Synonyms: yjcW

Regulation Summary Diagram: ?

Component of: D-allose ABC transporter (extended summary available)

Summary:
AlsA is the ATP-binding component of the allose ABC transporter.

Gene Citations: [Kim97, Poulsen99]

Locations: cytosol, inner membrane

Map Position: [4,307,471 <- 4,309,003] (92.84 centisomes)
Length: 1533 bp / 510 aa

Molecular Weight of Polypeptide: 56.745 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0013394 , DIP:DIP-9093N , EchoBASE:EB1902 , EcoGene:EG11959 , EcoliWiki:b4087 , ModBase:P32721 , OU-Microarray:b4087 , PortEco:alsA , PR:PRO_000022087 , Pride:P32721 , Protein Model Portal:P32721 , RefSeq:NP_418511 , RegulonDB:EG11959 , SMR:P32721 , String:511145.b4087 , UniProt:P32721

Relationship Links: EcoO157Cyc:Homolog:Z5691 , EcoO157Cyc:Homolog:Z5691-MONOMER , InterPro:IN-FAMILY:IPR003439 , InterPro:IN-FAMILY:IPR003593 , InterPro:IN-FAMILY:IPR017871 , InterPro:IN-FAMILY:IPR027417 , Pfam:IN-FAMILY:PF00005 , Prosite:IN-FAMILY:PS00211 , Prosite:IN-FAMILY:PS50893 , Smart:IN-FAMILY:SM00382

In Paralogous Gene Group: 23 (75 members)

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0006200 - ATP catabolic process Inferred by computational analysis [GOA01a]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a]
GO:0008152 - metabolic process Inferred by computational analysis [UniProtGOA11a]
GO:0008643 - carbohydrate transport Inferred by computational analysis [UniProtGOA11a]
GO:0015749 - monosaccharide transport Inferred by computational analysis [GOA01]
Molecular Function: GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11a]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0015407 - monosaccharide-transporting ATPase activity Inferred by computational analysis [GOA01]
GO:0016787 - hydrolase activity Inferred by computational analysis [UniProtGOA11a]
GO:0016887 - ATPase activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Zhang07]
GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a]

MultiFun Terms: metabolism carbon utilization carbon compounds
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily ATP binding cytoplasmic component

Essentiality data for alsA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of: D-allose ABC transporter

Subunit composition of D-allose ABC transporter = [AlsA]2[AlsC]2[AlsB]
         D-allose ABC transporter - ATP binding subunit = AlsA (summary available)
         D-allose ABC transporter - membrane subunit = AlsC (summary available)
         D-allose ABC transporter - periplasmic binding protein = AlsB (summary available)

Summary:
The AlsBAC transporter belongs to the ATP-binding Cassette (ABC) Superfamily [Saurin94]. It is responsible for the uptake of D-allose, an all-cis hexose that can be used by E. coli as a sole carbon source [Kim97]. Deletion of the als genes resulted in an inability to grow on D-allose [Kim97]. AlsB is a periplasmic protein that binds D-allose with a Kd of 0.33μM, as determined by fluorescence spectroscopy [Kim97]. Based on sequence similarity, AlsA is the ATP-binding component, and AlsC is the membrane component of the ABC transporter [Chaudhuri99]. The AlsBAC system also transports ribose at low affinity [Kim97]. Complementation analysis of als mutants showed that the cloned als genes could restore growth on ribose minimal media [Kim97]. Analysis of β-galactosidase transcriptional fusions suggest that AlsR is a negative regulator of alsABC, and transcription of alsR is regulated by allose [Kim97].


Enzymatic reaction of: D-allose ABC transporter

Synonyms: Transport of D-allose

Alternative Products for D-allose: β-D-ribopyranose [Kim97 ]


Sequence Features

Feature Class Location Citations Comment
Conserved-Region 6 -> 245
[UniProt09]
UniProt: ABC transporter 1;
Nucleotide-Phosphate-Binding-Region 38 -> 45
[UniProt10a]
UniProt: ATP; Non-Experimental Qualifier: potential;
Conserved-Region 260 -> 509
[UniProt09]
UniProt: ABC transporter 2;


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
10/20/97 Gene b4087 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11959; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Chaudhuri99: Chaudhuri BN, Ko J, Park C, Jones TA, Mowbray SL (1999). "Structure of D-allose binding protein from Escherichia coli bound to D-allose at 1.8 A resolution." J Mol Biol 1999;286(5);1519-31. PMID: 10064713

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Kim97: Kim C, Song S, Park C (1997). "The D-allose operon of Escherichia coli K-12." J Bacteriol 1997;179(24);7631-7. PMID: 9401019

Poulsen99: Poulsen TS, Chang YY, Hove-Jensen B (1999). "D-Allose catabolism of Escherichia coli: involvement of alsI and regulation of als regulon expression by allose and ribose." J Bacteriol 1999;181(22);7126-30. PMID: 10559180

Saurin94: Saurin W, Dassa E (1994). "Sequence relationships between integral inner membrane proteins of binding protein-dependent transport systems: evolution by recurrent gene duplications." Protein Sci 1994;3(2);325-44. PMID: 8003968

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Zhang07: Zhang N, Chen R, Young N, Wishart D, Winter P, Weiner JH, Li L (2007). "Comparison of SDS- and methanol-assisted protein solubilization and digestion methods for Escherichia coli membrane proteome analysis by 2-D LC-MS/MS." Proteomics 7(4);484-93. PMID: 17309111

Other References Related to Gene Regulation

Bennik00: Bennik MH, Pomposiello PJ, Thorne DF, Demple B (2000). "Defining a rob regulon in Escherichia coli by using transposon mutagenesis." J Bacteriol 182(13);3794-801. PMID: 10850996

Sorensen96: Sorensen KI, Hove-Jensen B (1996). "Ribose catabolism of Escherichia coli: characterization of the rpiB gene encoding ribose phosphate isomerase B and of the rpiR gene, which is involved in regulation of rpiB expression." J Bacteriol 1996;178(4);1003-11. PMID: 8576032

Zaslaver06: Zaslaver A, Bren A, Ronen M, Itzkovitz S, Kikoin I, Shavit S, Liebermeister W, Surette MG, Alon U (2006). "A comprehensive library of fluorescent transcriptional reporters for Escherichia coli." Nat Methods 3(8);623-8. PMID: 16862137


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Mon Dec 22, 2014, biocyc11.