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Escherichia coli K-12 substr. MG1655 Polypeptide: galactofuranose / galactospyranose ABC transporter - putative membrane subunit



Gene: yjfF Accession Numbers: EG12439 (EcoCyc), b4231, ECK4226

Regulation Summary Diagram: ?

Component of: galactofuranose / galactopyranose ABC transporter (summary available)

Summary:
YjfF is a putative membrane component of a galactose ABC transporter.

Gene Citations: [Maciag11]

Locations: inner membrane

Map Position: [4,451,606 -> 4,452,601] (95.95 centisomes)
Length: 996 bp / 331 aa

Molecular Weight of Polypeptide: 34.978 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0013840 , EchoBASE:EB2334 , EcoGene:EG12439 , EcoliWiki:b4231 , OU-Microarray:b4231 , PortEco:yjfF , Protein Model Portal:P37772 , RefSeq:NP_418652 , RegulonDB:EG12439 , String:511145.b4231 , UniProt:P37772

Relationship Links: InterPro:IN-FAMILY:IPR001851 , Pfam:IN-FAMILY:PF02653

In Paralogous Gene Group: 189 (15 members)

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0015757 - galactose transport Inferred from experiment [Horler09]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a, GOA01a]
Molecular Function: GO:0050782 - galactose uniporter activity Inferred from experiment [Horler09]
GO:0005215 - transporter activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a, Saurin99]
GO:0043190 - ATP-binding cassette (ABC) transporter complex Inferred by computational analysis [Saurin99]

MultiFun Terms: cell structure membrane
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily, membrane component

Essentiality data for yjfF knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of: galactofuranose / galactopyranose ABC transporter

Subunit composition of galactofuranose / galactopyranose ABC transporter = [YjfF][YtfR][YtfT]2[YtfQ]
         galactofuranose / galactospyranose ABC transporter - putative membrane subunit = YjfF (summary available)
         galactofuranose / galactopyranose ABC transporter - putative ATP binding subunit = YtfR (summary available)
         galactofuranose / galactopyranose ABC transporter - putative membrane subunit = YtfT (summary available)
         galactofuranose / galactopyranose ABC transporter - periplasmic binding protein = YtfQ (summary available)

Summary:
YtfQRT/YjfF is a galactofuranose and galactopyranose transporter that is a member of the ATP-Binding Cassette (ABC) superfamily of transporters. YtfR is the putative ATP-binding component. YtfT and YjfF are the putative membrane components. YtfQ is the periplasmic binding protein [Horler09].

Locations: inner membrane

GO Terms:

Biological Process: GO:0015757 - galactose transport Inferred from experiment [Horler09]
Molecular Function: GO:0005354 - galactose transmembrane transporter activity Inferred from experiment [Horler09]
GO:0015407 - monosaccharide-transporting ATPase activity Inferred by computational analysis [Saurin99]
Cellular Component: GO:0043190 - ATP-binding cassette (ABC) transporter complex Inferred by computational analysis [Saurin99]


Enzymatic reaction of: transport of β-D-galactofuranose (galactofuranose / galactopyranose ABC transporter)


Enzymatic reaction of: transport of α-D-galactofuranose (galactofuranose / galactopyranose ABC transporter)

EC Number: 3.6.3.-


Enzymatic reaction of: transport of β-D-galactose (galactofuranose / galactopyranose ABC transporter)

EC Number: 3.6.3.17


Sequence Features

Feature Class Location Citations Comment
Transmembrane-Region 6 -> 26
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 43 -> 63
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 65 -> 85
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 89 -> 109
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 114 -> 134
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 160 -> 180
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 223 -> 243
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 252 -> 272
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Sequence-Conflict 257
[Hamilton88a, UniProt10]
Alternate sequence: L → V; UniProt: (in Ref. 4; X12545);
Transmembrane-Region 295 -> 315
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Peter D. Karp on Wed Jan 18, 2006:
Gene left-end position adjusted based on analysis performed in the 2005 E. coli annotation update [Riley06 ].
10/20/97 Gene b4231 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12439; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Hamilton88a: Hamilton WD, Harrison DA, Dyer TA (1988). "Sequence of the Escherichia coli fructose-1,6-bisphosphatase gene." Nucleic Acids Res 1988;16(17);8707. PMID: 2843822

Horler09: Horler RS, Muller A, Williamson DC, Potts JR, Wilson KS, Thomas GH (2009). "Furanose-specific sugar transport: characterization of a bacterial galactofuranose-binding protein." J Biol Chem 284(45);31156-63. PMID: 19744923

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Maciag11: Maciag A, Peano C, Pietrelli A, Egli T, De Bellis G, Landini P (2011). "In vitro transcription profiling of the {sigma}S subunit of bacterial RNA polymerase: re-definition of the {sigma}S regulon and identification of {sigma}S-specific promoter sequence elements." Nucleic Acids Res 39(13);5338-55. PMID: 21398637

Riley06: Riley M, Abe T, Arnaud MB, Berlyn MK, Blattner FR, Chaudhuri RR, Glasner JD, Horiuchi T, Keseler IM, Kosuge T, Mori H, Perna NT, Plunkett G, Rudd KE, Serres MH, Thomas GH, Thomson NR, Wishart D, Wanner BL (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34(1);1-9. PMID: 16397293

Saurin99: Saurin W, Hofnung M, Dassa E (1999). "Getting in or out: early segregation between importers and exporters in the evolution of ATP-binding cassette (ABC) transporters." J Mol Evol 1999;48(1);22-41. PMID: 9873074

UniProt10: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Stringer14: Stringer AM, Currenti S, Bonocora RP, Baranowski C, Petrone BL, Palumbo MJ, Reilly AA, Zhang Z, Erill I, Wade JT (2014). "Genome-scale analyses of Escherichia coli and Salmonella enterica AraC reveal noncanonical targets and an expanded core regulon." J Bacteriol 196(3);660-71. PMID: 24272778


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 28, 2014, BIOCYC13B.