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Escherichia coli K-12 substr. MG1655 Polypeptide: putative transport protein, ABC superfamily - ATP-binding component



Gene: yphE Accession Numbers: G7341 (EcoCyc), b2547, ECK2544

Regulation Summary Diagram: ?

Regulation summary diagram for yphE

Component of: putative transport complex, ABC superfamily (summary available)

Summary:
YphE is an ATP-binding component of a predicted ATP-dependent sugar transporter.

Locations: inner membrane, cytosol

Map Position: [2,674,872 <- 2,676,383] (57.65 centisomes, 208°)
Length: 1512 bp / 503 aa

Molecular Weight of Polypeptide: 55.016 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0008376 , DIP:DIP-12830N , EchoBASE:EB3239 , EcoGene:EG13466 , EcoliWiki:b2547 , ModBase:P77509 , OU-Microarray:b2547 , PortEco:yphE , Pride:P77509 , Protein Model Portal:P77509 , RefSeq:NP_417042 , RegulonDB:G7341 , SMR:P77509 , String:511145.b2547 , UniProt:P77509

Relationship Links: InterPro:IN-FAMILY:IPR003439 , InterPro:IN-FAMILY:IPR003593 , InterPro:IN-FAMILY:IPR017871 , InterPro:IN-FAMILY:IPR027417 , Pfam:IN-FAMILY:PF00005 , Prosite:IN-FAMILY:PS00211 , Prosite:IN-FAMILY:PS50893 , Smart:IN-FAMILY:SM00382

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
GO:0008152 - metabolic process Inferred by computational analysis [GOA01]
Molecular Function: GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0016887 - ATPase activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0005886 - plasma membrane Inferred by computational analysis [DiazMejia09]

MultiFun Terms: transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily ATP binding cytoplasmic component

Essentiality data for yphE knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Subunit of: putative transport complex, ABC superfamily

Subunit composition of putative transport complex, ABC superfamily = [YphD][YphF][YphE]
         putative transport protein, ABC superfamily - membrane component = YphD (summary available)
         putative transport protein, ABC superfamily - periplasmic binding protein = YphF (summary available)
         putative transport protein, ABC superfamily - ATP-binding component = YphE (summary available)

Summary:
YphE, YphD and YphF are uncharacterized members of the ABC superfamily of transporters [Saurin99]. YphE is the putative ATP-binding component; YphD is the putative membrane component; YphF is the putative periplasmic binding component. The yphE, yphD and yphF genes are probably located in a single operon; these proteins may function together as an ATP-dependent sugar transporter


Sequence Features

Protein sequence of putative transport protein, ABC superfamily - ATP-binding component with features indicated

Feature Class Location Citations Comment
Conserved-Region 8 -> 249
[UniProt09]
UniProt: ABC transporter 1;
Nucleotide-Phosphate-Binding-Region 43 -> 50
[UniProt10]
UniProt: ATP; Non-Experimental Qualifier: potential;
Conserved-Region 261 -> 499
[UniProt09]
UniProt: ABC transporter 2;


Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram

Notes:

History:
Markus Krummenacker on Tue Oct 14, 1997:
Gene object created from Blattner lab Genbank (v. M52) entry.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Saurin99: Saurin W, Hofnung M, Dassa E (1999). "Getting in or out: early segregation between importers and exporters in the evolution of ATP-binding cassette (ABC) transporters." J Mol Evol 1999;48(1);22-41. PMID: 9873074

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Thu Aug 27, 2015, BIOCYC14B.