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Escherichia coli K-12 substr. MG1655 Transporter: glutathione ABC transporter

Subunit composition of glutathione ABC transporter = [GsiA]2[GsiB][GsiD][GsiC]
         glutathione ABC transporter - ATP binding subunit = GsiA (summary available)
         glutathione ABC transporter - periplasmic binding protein = GsiB (summary available)
         glutathione ABC transporter - membrane subunit = GsiD (summary available)
         glutathione ABC transporter - membrane subunit = GsiC (summary available)

Summary:
The GsiABCD glutathione transporter is a member of the ATP Binding Cassette (ABC) superfamily of transporters. Based on sequence similarity, GsiA is predicted to be the ATP-binding component, GsiB is predicted to be the periplasmic binding component, and GsiC and GsiD are predicted to be inner membrane components. Mutation of any component impairs the ability of E. coli to transport the glutathione molecule into the cell. Expression of the cloned genes in their respective mutants restores their ability to transport glutathione. Transport by the glutathione ABC transporter is one of two known mechanisms for salvage of glutathione excreted from the cell. The other involves hydrolysis of glutathione by γ-glutamyl transpeptidase in the periplasm to yield glutamic acid and cysteinylglycine which can be taken back into the cell [Suzuki05a].

Locations: inner membrane

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0034775 - glutathione transmembrane transport Inferred from experiment [Suzuki05a]
Molecular Function: GO:0034634 - glutathione transmembrane transporter activity Inferred from experiment [Suzuki05a]
GO:0043492 - ATPase activity, coupled to movement of substances Inferred from experiment [Suzuki05a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment [Suzuki05a]


Enzymatic reaction of: transport of glutathione (glutathione ABC transporter)


Subunit of glutathione ABC transporter: glutathione ABC transporter - ATP binding subunit

Synonyms: GsiA, YliA

Gene: gsiA Accession Numbers: G6429 (EcoCyc), b0829, ECK0819

Locations: inner membrane

Sequence Length: 612 AAs

Molecular Weight: 67.979 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0006200 - ATP catabolic process Inferred by computational analysis Inferred from experiment [Suzuki05a, GOA01a]
GO:0034775 - glutathione transmembrane transport Inferred from experiment [Suzuki05a]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a]
GO:0008152 - metabolic process Inferred by computational analysis [UniProtGOA11a]
GO:0015833 - peptide transport Inferred by computational analysis [GOA01a]
Molecular Function: GO:0043492 - ATPase activity, coupled to movement of substances Inferred from experiment [Suzuki05a]
GO:0000166 - nucleotide binding Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0005524 - ATP binding Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0016787 - hydrolase activity Inferred by computational analysis [UniProtGOA11a]
GO:0016887 - ATPase activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Zhang07]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a]

MultiFun Terms: transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily ATP binding cytoplasmic component

Unification Links: EcoliWiki:b0829 , ModBase:P75796 , PR:PRO_000022844 , Protein Model Portal:P75796 , RefSeq:NP_415350 , SMR:P75796 , String:511145.b0829 , UniProt:P75796

Relationship Links: InterPro:IN-FAMILY:IPR003439 , InterPro:IN-FAMILY:IPR003593 , InterPro:IN-FAMILY:IPR013563 , InterPro:IN-FAMILY:IPR017871 , InterPro:IN-FAMILY:IPR027417 , Pfam:IN-FAMILY:PF00005 , Pfam:IN-FAMILY:PF08352 , Prosite:IN-FAMILY:PS00211 , Prosite:IN-FAMILY:PS50893 , Smart:IN-FAMILY:SM00382

Summary:
ATP-binding component of glutathione ABC transporter

Essentiality data for gsiA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]

Subunit of glutathione ABC transporter: glutathione ABC transporter - periplasmic binding protein

Synonyms: GsiB, YliB

Gene: gsiB Accession Numbers: G6430 (EcoCyc), b0830, ECK0820

Locations: periplasmic space

Sequence Length: 512 AAs

Molecular Weight: 56.47 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0006974 - cellular response to DNA damage stimulus Inferred from experiment [Khil02]
GO:0034775 - glutathione transmembrane transport Inferred from experiment [Suzuki05a]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a, GOA01a]
Molecular Function: GO:0034634 - glutathione transmembrane transporter activity Inferred from experiment [Suzuki05a]
GO:0005215 - transporter activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0030288 - outer membrane-bounded periplasmic space Inferred from experiment Inferred by computational analysis [DiazMejia09, Han13, LopezCampistrou05]
GO:0042597 - periplasmic space Inferred by computational analysis [UniProtGOA11, UniProtGOA11a]

MultiFun Terms: transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily, periplasmic binding component

Unification Links: DIP:DIP-12697N , EcoliWiki:b0830 , ModBase:P75797 , PR:PRO_000022845 , Pride:P75797 , Protein Model Portal:P75797 , RefSeq:NP_415351 , SMR:P75797 , String:511145.b0830 , UniProt:P75797

Relationship Links: InterPro:IN-FAMILY:IPR000914 , InterPro:IN-FAMILY:IPR023765 , PDB:Structure:1UQW , Pfam:IN-FAMILY:PF00496 , Prosite:IN-FAMILY:PS01040

Summary:
periplasmic binding component of glutathione ABC transporter

Essentiality data for gsiB knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of glutathione ABC transporter: glutathione ABC transporter - membrane subunit

Synonyms: GsiD, YliD

Gene: gsiD Accession Numbers: G6432 (EcoCyc), b0832, ECK0822

Locations: inner membrane

Sequence Length: 303 AAs

Molecular Weight: 33.238 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0034775 - glutathione transmembrane transport Inferred from experiment [Suzuki05a]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a, GOA01a]
Molecular Function: GO:0034634 - glutathione transmembrane transporter activity Inferred from experiment [Suzuki05a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a]

MultiFun Terms: cell structure membrane
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily, membrane component

Unification Links: EcoliWiki:b0832 , PR:PRO_000022847 , Protein Model Portal:P75799 , RefSeq:NP_415353 , SMR:P75799 , String:511145.b0832 , UniProt:P75799

Relationship Links: InterPro:IN-FAMILY:IPR000515 , InterPro:IN-FAMILY:IPR025966 , Pfam:IN-FAMILY:PF00528 , Pfam:IN-FAMILY:PF12911 , Prosite:IN-FAMILY:PS50928

Summary:
inner membrane component of glutathione ABC transporter

Essentiality data for gsiD knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of glutathione ABC transporter: glutathione ABC transporter - membrane subunit

Synonyms: GsiC, YliC

Gene: gsiC Accession Numbers: G6431 (EcoCyc), b0831, ECK0821

Locations: inner membrane

Sequence Length: 306 AAs

Molecular Weight: 34.066 kD (from nucleotide sequence)

GO Terms:

Biological Process: GO:0034775 - glutathione transmembrane transport Inferred from experiment [Suzuki05a]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a, GOA01a]
Molecular Function: GO:0034634 - glutathione transmembrane transporter activity Inferred from experiment [Suzuki05a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Zhang07, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a]

MultiFun Terms: transport Putative uncharacterized transport protein

Unification Links: EcoliWiki:b0831 , PR:PRO_000022846 , Protein Model Portal:P75798 , RefSeq:NP_415352 , String:511145.b0831 , UniProt:P75798

Relationship Links: InterPro:IN-FAMILY:IPR000515 , Pfam:IN-FAMILY:PF00528 , Prosite:IN-FAMILY:PS50928

Summary:
inner membrane component of glutathione ABC transporter

Essentiality data for gsiC knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Han13: Han MJ, Kim JY, Kim JA (2013). "Comparison of the large-scale periplasmic proteomes of the Escherichia coli K-12 and B strains." J Biosci Bioeng. PMID: 24140104

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Khil02: Khil PP, Camerini-Otero RD (2002). "Over 1000 genes are involved in the DNA damage response of Escherichia coli." Mol Microbiol 44(1);89-105. PMID: 11967071

LopezCampistrou05: Lopez-Campistrous A, Semchuk P, Burke L, Palmer-Stone T, Brokx SJ, Broderick G, Bottorff D, Bolch S, Weiner JH, Ellison MJ (2005). "Localization, annotation, and comparison of the Escherichia coli K-12 proteome under two states of growth." Mol Cell Proteomics 4(8);1205-9. PMID: 15911532

Suzuki05a: Suzuki H, Koyanagi T, Izuka S, Onishi A, Kumagai H (2005). "The yliA, -B, -C, and -D Genes of Escherichia coli K-12 Encode a Novel Glutathione Importer with an ATP-Binding Cassette." J Bacteriol 187(17);5861-7. PMID: 16109926

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Zhang07: Zhang N, Chen R, Young N, Wishart D, Winter P, Weiner JH, Li L (2007). "Comparison of SDS- and methanol-assisted protein solubilization and digestion methods for Escherichia coli membrane proteome analysis by 2-D LC-MS/MS." Proteomics 7(4);484-93. PMID: 17309111


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 28, 2014, BIOCYC14B.