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Escherichia coli K-12 substr. MG1655 Transporter: branched chain amino acid transporter BrnQ



Gene: brnQ Accession Numbers: EG12168 (EcoCyc), b0401, ECK0395

Synonyms: hrbA, LIV-II, BrnQ branched chain amino acid LIVCS transporter

Regulation Summary Diagram: ?

Summary:
BrnQ is a probable branched chain amino acid transporter. BrnQ is highly similar to the Salmonella typhimurium BrnQ branched chain amino acid transporter [Ohnishi88] and very probably corresponds to the Liv-II branched chain amino acid transport system in E. coli, which has been shown to transport leucine, valine, and isoleucine [Guardiola74, Guardiola71, Anderson78]. BrnQ is a member of the LIVCS family of branched chain amino acid transporters and probably functions as a sodium/branched chain amino acid symporter.

Locations: inner membrane

Map Position: [418,815 -> 420,134] (9.03 centisomes)
Length: 1320 bp / 439 aa

Molecular Weight of Polypeptide: 46.209 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0001394 , CGSC:950 , DIP:DIP-48078N , EchoBASE:EB2087 , EcoGene:EG12168 , EcoliWiki:b0401 , Mint:MINT-1238115 , OU-Microarray:b0401 , PortEco:brnQ , PR:PRO_000022231 , Pride:P0AD99 , Protein Model Portal:P0AD99 , RefSeq:NP_414935 , RegulonDB:EG12168 , String:511145.b0401 , UniProt:P0AD99

Relationship Links: InterPro:IN-FAMILY:IPR004685 , Pfam:IN-FAMILY:PF05525

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0015803 - branched-chain amino acid transport Inferred from experiment Inferred by computational analysis [GOA01a, Guardiola74]
GO:0015807 - L-amino acid transport Inferred from experiment [Guardiola71, Guardiola74]
GO:0015818 - isoleucine transport Inferred from experiment [Guardiola71, Guardiola74]
GO:0015820 - leucine transport Inferred from experiment [Guardiola71, Guardiola74]
GO:0015829 - valine transport Inferred from experiment [Guardiola71, Guardiola74]
GO:0098655 - cation transmembrane transport Inferred from experiment [Guardiola71, Guardiola74]
GO:1902475 - L-alpha-amino acid transmembrane transport Inferred from experiment [Guardiola71, Guardiola74]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a]
GO:0006865 - amino acid transport Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0005304 - L-valine transmembrane transporter activity Inferred from experiment [Guardiola71, Guardiola74]
GO:0005515 - protein binding Inferred from experiment [Rajagopala14, Butland05]
GO:0015188 - L-isoleucine transmembrane transporter activity Inferred from experiment [Guardiola74, Guardiola71]
GO:0015190 - L-leucine transmembrane transporter activity Inferred from experiment [Guardiola71, Guardiola74]
GO:0015658 - branched-chain amino acid transmembrane transporter activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05, Yamato79]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]

MultiFun Terms: cell structure membrane
metabolism biosynthesis of building blocks amino acids isoleucine/valine
metabolism biosynthesis of building blocks amino acids leucine
metabolism carbon utilization amino acids
transport Electrochemical potential driven transporters Porters (Uni-, Sym- and Antiporters)

Essentiality data for brnQ knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Enzymatic reaction of: branched chain amino acid transporter BrnQ

Synonyms: Transport of L-leucine

Summary:
The reaction catalysed by BrnQ is shown as sodium:leucine symport but this has not been experimentally characterised. The LIVCS family of which BrnQ is a member catalyses the general reaction:
[L, I or V] (out) + [Na+ or H+] (out) --> [L, I or V] (in) + [Na+ or H+] (in)


Enzymatic reaction of: branched chain amino acid transporter BrnQ

Synonyms: Transport of L-valine

Summary:
The reaction catalysed by BrnQ is shown as sodium:valine symport but this has not been experimentally characterised. The LIVCS family of which BrnQ is a member catalyses the general reaction:
[L, I or V] (out) + [Na+ or H+] (out) --> [L, I or V] (in) + [Na+ or H+] (in)


Enzymatic reaction of: branched chain amino acid transporter BrnQ

Synonyms: Transport of L-isoleucine

Summary:
The reaction catalysed by BrnQ is shown as sodium:isoleucine symport but this has not been experimentally characterised. The LIVCS family of which BrnQ is a member catalyses the general reaction:
[L, I or V] (out) + [Na+ or H+] (out) --> [L, I or V] (in) + [Na+ or H+] (in)


Sequence Features

Feature Class Location Citations Comment
Transmembrane-Region 10 -> 30
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 46 -> 66
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 80 -> 100
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 119 -> 139
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 150 -> 170
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 190 -> 210
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 227 -> 247
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 278 -> 298
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 317 -> 337
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 339 -> 359
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 370 -> 390
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 405 -> 425
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b0401 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12168; confirmed by SwissProt match.


References

Anderson78: Anderson JJ, Oxender DL (1978). "Genetic separation of high- and low-affinity transport systems for branched-chain amino acids in Escherichia coli K-12." J Bacteriol 1978;136(1);168-74. PMID: 361686

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Butland05: Butland G, Peregrin-Alvarez JM, Li J, Yang W, Yang X, Canadien V, Starostine A, Richards D, Beattie B, Krogan N, Davey M, Parkinson J, Greenblatt J, Emili A (2005). "Interaction network containing conserved and essential protein complexes in Escherichia coli." Nature 433(7025);531-7. PMID: 15690043

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Guardiola71: Guardiola J, Iaccarino M (1971). "Escherichia coli K-12 mutants altered in the transport of branched-chain amino acids." J Bacteriol 108(3);1034-44. PMID: 4945181

Guardiola74: Guardiola J, De Felice M, Klopotowski T, Iaccarino M (1974). "Mutations affecting the different transport systems for isoleucine, leucine, and valine in Escherichia coli K-12." J Bacteriol 117(2);393-405. PMID: 4590465

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Ohnishi88: Ohnishi K, Hasegawa A, Matsubara K, Date T, Okada T, Kiritani K (1988). "Cloning and nucleotide sequence of the brnQ gene, the structural gene for a membrane-associated component of the LIV-II transport system for branched-chain amino acids in Salmonella typhimurium." Jpn J Genet 1988;63(4);343-57. PMID: 3078876

Rajagopala14: Rajagopala SV, Sikorski P, Kumar A, Mosca R, Vlasblom J, Arnold R, Franca-Koh J, Pakala SB, Phanse S, Ceol A, Hauser R, Siszler G, Wuchty S, Emili A, Babu M, Aloy P, Pieper R, Uetz P (2014). "The binary protein-protein interaction landscape of Escherichia coli." Nat Biotechnol 32(3);285-90. PMID: 24561554

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Yamato79: Yamato I, Ohki M, Anraku Y (1979). "Genetic and biochemical studies of transport systems for branched-chain amino acids in Escherichia coli." J Bacteriol 138(1);24-32. PMID: 374366

Other References Related to Gene Regulation

MendozaVargas09: Mendoza-Vargas A, Olvera L, Olvera M, Grande R, Vega-Alvarado L, Taboada B, Jimenez-Jacinto V, Salgado H, Juarez K, Contreras-Moreira B, Huerta AM, Collado-Vides J, Morett E (2009). "Genome-wide identification of transcription start sites, promoters and transcription factor binding sites in E. coli." PLoS One 4(10);e7526. PMID: 19838305


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Thu Nov 27, 2014, biocyc12.