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Escherichia coli K-12 substr. MG1655 Polypeptide: ZraS sensory histidine kinase



Gene: zraS Accession Numbers: EG10008 (EcoCyc), b4003, ECK3995

Synonyms: hydH

Regulation Summary Diagram: ?

Alternative forms of ZraS sensory histidine kinase: ZraS sensory histidine kinase - phosphorylated (summary available)

Summary:
ZraS is the sensory histidine kinase of the two component signal transduction system ZraS/ZraR which regulates the expression of zraP - a periplasmic zinc-binding protein [Leonhartsberger01]. ZraS is an inner membrane protein with two predicted transmembrane segments [Leonhartsberger01]. Zinc dependent regulation of zraP expression requires a functional zraS gene [Leonhartsberger01]. The expression of zraS is positively autoregulated with zinc as an effector [Leonhartsberger01]. Autophosphorylation of the purifed cytoplasmic domain of ZraS and subsequent transfer of the phosphoryl group to ZraR has been demonstrated in vitro [Yamamoto05]. A metal binding site in ZraS has not been identified.

Gene Citations: [Stoker89]

Locations: inner membrane

Map Position: [4,199,949 -> 4,201,346] (90.52 centisomes)
Length: 1398 bp / 465 aa

Molecular Weight of Polypeptide: 51.032 kD (from nucleotide sequence)

pI: 8.92

Unification Links: ASAP:ABE-0013088 , CGSC:34249 , DIP:DIP-9981N , EchoBASE:EB0008 , EcoGene:EG10008 , EcoliWiki:b4003 , ModBase:P14377 , OU-Microarray:b4003 , PortEco:zraS , PR:PRO_000024257 , Pride:P14377 , Protein Model Portal:P14377 , RegulonDB:EG10008 , SMR:P14377 , String:511145.b4003 , UniProt:P14377

Relationship Links: InterPro:IN-FAMILY:IPR003594 , InterPro:IN-FAMILY:IPR003661 , InterPro:IN-FAMILY:IPR004358 , InterPro:IN-FAMILY:IPR005467 , InterPro:IN-FAMILY:IPR009082 , Pfam:IN-FAMILY:PF00512 , Pfam:IN-FAMILY:PF02518 , Prints:IN-FAMILY:PR00344 , Prosite:IN-FAMILY:PS50109 , Smart:IN-FAMILY:SM00387 , Smart:IN-FAMILY:SM00388

In Paralogous Gene Group: 122 (29 members)

Reactions known to consume the compound:

ZraSR Two-Component Signal Transduction System :
ZraS[inner membrane] + ATP → ZraS-Phis254[inner membrane] + ADP

Reactions known to produce the compound:

ZraSR Two-Component Signal Transduction System :
ZraR + ZraS-Phis254[inner membrane] → ZraR-Pasp56 + ZraS[inner membrane]

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0000160 - phosphorelay signal transduction system Inferred from experiment Inferred by computational analysis [UniProt-GOA, 2011, Yamamoto05]
GO:0023014 - signal transduction by phosphorylation Inferred by computational analysis Inferred from experiment [Leonhartsberger01, GOA et al., 2001]
GO:0046777 - protein autophosphorylation Inferred from experiment [Yamamoto05]
GO:0071284 - cellular response to lead ion Inferred from experiment [Leonhartsberger01]
GO:0071294 - cellular response to zinc ion Inferred from experiment [Leonhartsberger01]
GO:0007165 - signal transduction Inferred by computational analysis [GOA et al., 2001]
GO:0016310 - phosphorylation Inferred by computational analysis [UniProt-GOA, 2011, GOA et al., 2001]
GO:0018106 - peptidyl-histidine phosphorylation Inferred by computational analysis [GOA01]
Molecular Function: GO:0000155 - phosphorelay sensor kinase activity Inferred from experiment Inferred by computational analysis [GOA et al., 2001, Leonhartsberger01]
GO:0000166 - nucleotide binding Inferred by computational analysis [UniProt-GOA, 2011]
GO:0004673 - protein histidine kinase activity Inferred by computational analysis [GOA01]
GO:0004871 - signal transducer activity Inferred by computational analysis [GOA et al., 2001]
GO:0005524 - ATP binding Inferred by computational analysis [UniProt-GOA, 2011]
GO:0016301 - kinase activity Inferred by computational analysis [UniProt-GOA, 2011]
GO:0016740 - transferase activity Inferred by computational analysis [UniProt-GOA, 2011]
GO:0016772 - transferase activity, transferring phosphorus-containing groups Inferred by computational analysis [GOA et al., 2001]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProt-GOA, 2011, Diaz-Mejia et al., 2009, Daley05]
GO:0005887 - integral component of plasma membrane Inferred from experiment [Leonhartsberger01]
GO:0016020 - membrane Inferred from experiment Inferred by computational analysis [UniProt-GOA, 2011, GOA et al., 2001, Leonhartsberger01]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProt-GOA, 2011]

MultiFun Terms: cell structure membrane
information transfer protein related posttranslational modification
metabolism energy metabolism, carbon fermentation
regulation type of regulation posttranscriptional covalent modification, demodification, maturation
regulation type of regulation transcriptional level complex regulation two component regulatory systems (external signal)

Essentiality data for zraS knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes et al., 2003, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba et al., 2006, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce et al., 2006, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba et al., 2006, Comment 2]

Credits:
Last-Curated ? 29-Nov-2010 by Mackie A , Macquarie University


Sequence Features

Feature Class Location Citations Comment State
Transmembrane-Region 15 -> 35
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
 
Transmembrane-Region 202 -> 222
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
 
Conserved-Region 251 -> 458
[UniProt, 2009]
UniProt: Histidine kinase;
 
Phosphorylation-Modification 254
[UniProt13]
UniProt: Phosphohistidine; by autocatalysis; Non-Experimental Qualifier: by similarity.
Unmodified
Sequence-Conflict 361
[Stoker89, UniProt, 2010]
Alternate sequence: L → V; UniProt: (in Ref. 4; AAA24003);
 
Sequence-Conflict 383 -> 387
[Stoker89, UniProt, 2010]
Alternate sequence: SESGA → TKAG; UniProt: (in Ref. 4; AAA24003);
 


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
10/20/97 Gene b4003 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10008.


References

Baba et al., 2006: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

Diaz-Mejia et al., 2009: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes et al., 2003: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA et al., 2001: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

Joyce et al., 2006: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Leonhartsberger01: Leonhartsberger S, Huber A, Lottspeich F, Bock A (2001). "The hydH/G Genes from Escherichia coli code for a zinc and lead responsive two-component regulatory system." J Mol Biol 2001;307(1);93-105. PMID: 11243806

Stoker89: Stoker K, Reijnders WN, Oltmann LF, Stouthamer AH (1989). "Initial cloning and sequencing of hydHG, an operon homologous to ntrBC and regulating the labile hydrogenase activity in Escherichia coli K-12." J Bacteriol 1989;171(8);4448-56. PMID: 2666400

UniProt, 2009: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt, 2010: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt-GOA, 2011: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt13: UniProt Consortium (2013). "UniProt version 2013-08 released on 2013-08-01 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Yamamoto05: Yamamoto K, Hirao K, Oshima T, Aiba H, Utsumi R, Ishihama A (2005). "Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli." J Biol Chem 280(2);1448-56. PMID: 15522865

Other References Related to Gene Regulation

Barrios99: Barrios H, Valderrama B, Morett E (1999). "Compilation and analysis of sigma(54)-dependent promoter sequences." Nucleic Acids Res 27(22);4305-13. PMID: 10536136

Reitzer01: Reitzer L, Schneider BL (2001). "Metabolic context and possible physiological themes of sigma(54)-dependent genes in Escherichia coli." Microbiol Mol Biol Rev 65(3);422-44, table of contents. PMID: 11528004

Zheng04: Zheng D, Constantinidou C, Hobman JL, Minchin SD (2004). "Identification of the CRP regulon using in vitro and in vivo transcriptional profiling." Nucleic Acids Res 32(19);5874-93. PMID: 15520470


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Thu Dec 18, 2014, biocyc11.