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Escherichia coli K-12 substr. MG1655 Polypeptide: osmotically inducible protein OsmE

Gene: osmE Accession Numbers: EG10044 (EcoCyc), b1739, ECK1737

Synonyms: anr

Regulation Summary Diagram: ?

Regulation summary diagram for osmE

OsmE is an osmotically inducible gene product in Escherichia coli . Operon fusion experiments demonstrated that osmE osmotic regulation probably occurs at the transcriptional level [Gutierrez95]. Under conditions of low osmolarity, osmE is induced at the onset of stationary phase [Conter97]. In the absence of sigma s, the stationary phase sigma factor encoded by rpoS, stationary phase induction does not occur but the osmotic effect is still observed [Conter97]. OsmE has a lipoprotein-type signal sequence at the amino terminus [Gutierrez95].

Locations: inner membrane

Map Position: [1,819,942 <- 1,820,280] (39.23 centisomes, 141°)
Length: 339 bp / 112 aa

Molecular Weight of Polypeptide: 12.021 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0005799 , CGSC:36628 , EchoBASE:EB0042 , EcoGene:EG10044 , EcoliWiki:b1739 , OU-Microarray:b1739 , PortEco:osmE , PR:PRO_000023469 , Pride:P0ADB1 , Protein Model Portal:P0ADB1 , RefSeq:NP_416253 , RegulonDB:EG10044 , SMR:P0ADB1 , String:511145.b1739 , UniProt:P0ADB1

Relationship Links: InterPro:IN-FAMILY:IPR007450 , Pfam:IN-FAMILY:PF04355 , Prosite:IN-FAMILY:PS51257

Genetic Regulation Schematic: ?

Genetic regulation schematic for osmE

GO Terms:

Biological Process: GO:0006970 - response to osmotic stress Inferred from experiment [Conter97]
Molecular Function: GO:0005515 - protein binding Inferred from experiment [Zheng11]
Cellular Component: GO:0005886 - plasma membrane Inferred by computational analysis [UniProtGOA11a, UniProtGOA11]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11]
GO:0019867 - outer membrane Inferred by computational analysis [GOA01]

MultiFun Terms: cell processes adaptations osmotic pressure
information transfer RNA related Transcription related
regulation genetic unit regulated operon
regulation type of regulation transcriptional level activator

Essentiality data for osmE knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Sequence Features

Protein sequence of osmotically inducible protein OsmE with features indicated

Feature Class Location Citations Comment
Signal-Sequence 1 -> 20
UniProt: Non-Experimental Qualifier: probable;
Lipid-Binding-Site 21
UniProt: N-palmitoyl cysteine; Non-Experimental Qualifier: probable;
Chain 21 -> 112
UniProt: Osmotically-inducible lipoprotein E;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


10/20/97 Gene b1739 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10044; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Conter97: Conter A, Menchon C, Gutierrez C (1997). "Role of DNA supercoiling and rpoS sigma factor in the osmotic and growth phase-dependent induction of the gene osmE of Escherichia coli K12." J Mol Biol 1997;273(1);75-83. PMID: 9367747

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Gutierrez95: Gutierrez C, Gordia S, Bonnassie S (1995). "Characterization of the osmotically inducible gene osmE of Escherichia coli K-12." Mol Microbiol 1995;16(3);553-63. PMID: 7565114

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Zheng11: Zheng C, Yang L, Hoopmann MR, Eng JK, Tang X, Weisbrod CR, Bruce JE (2011). "Cross-linking measurements of in vivo protein complex topologies." Mol Cell Proteomics 10(10);M110.006841. PMID: 21697552

Other References Related to Gene Regulation

Bordes00: Bordes P, Repoila F, Kolb A, Gutierrez C (2000). "Involvement of differential efficiency of transcription by esigmas and esigma70 RNA polymerase holoenzymes in growth phase regulation of the Escherichia coli osmE promoter." Mol Microbiol 35(4);845-53. PMID: 10692161

Bordes02: Bordes P, Bouvier J, Conter A, Kolb A, Gutierrez C (2002). "Transient repressor effect of Fis on the growth phase-regulated osmE promoter of Escherichia coli K12." Mol Genet Genomics 268(2);206-13. PMID: 12395194

Ishida76: Ishida Y, Watanabe K, Kobayashi S, Kihar M (1976). "Selective alpha-adrenoceptor blocking actions of a new derivative of 2-halogenotheylamine: 6-(2-bromoethyl)-10,11-methylenedioxy-5,6,7,8-tetrahydrodibenz[c,e]azocine." Jpn J Pharmacol 26(5);607-14. PMID: 12395

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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