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Escherichia coli K-12 substr. MG1655 Transporter: β-glucoside PTS permease AscF (cryptic)



Gene: ascF Accession Numbers: EG10086 (EcoCyc), b2715, ECK2710

Synonyms: sac, EIIBCasc, Enzyme IIBCasc

Regulation Summary Diagram: ?

Subunit composition of β-glucoside PTS permease AscF (cryptic) = [AscF]2

Summary:
AscF belongs to the functional superfamily of the phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system (PTSsugar). The PTSsugar transports and simultaneously phosphorylates its sugar sustrates in a process known as group translocation (reviewed in [Postma93].

When activated (see below) ascF encodes an Enzyme IIBCAsc complex. AscF possesses two domains in a single polypeptide chain with the domain order IIB-IIC. It is homologous to PtsG (the glucose-specific PTS Enzyme II) which has been reported to possess 8 transmembrane α-helical segments in its IIC domain. In order for transport to occur, a IIA domain from another PTS transport system, such as IIAGlc, would be required to transfer the phosphate from HPr to IIBAsc [Hall92a]. The specific IIA domain that associates with the cryptic β-glucoside PTS transporter has not been identified. AscF is a member of the PTS Glucose-Glucoside family of transporters [Saier14].

The asc operon contains the ascF gene encoding the Enzyme IIBCAsc and the ascB gene encoding a phospho-β-glucosidase that hydrolyzes the aglycone from the glycoside phosphate ester [Hall92a]. Monocistronic ascG, encoding a transcriptional repressor of the ascFB operon, and the ascFB operon are transcribed from divergent promoters [Hall92a].

The asc operon is cryptic in wild type E. coli K-12 [Parker88, Ishida09]. It can be activated by the insertion of IS186 into ascG in some E. coli isolates [Hall92a]. Crypticity of this and other E. coli β-glucoside metabolic operons presumably serves as a protective device against toxic β-glucosides found in nature [Hall92a]. Decryptified asc mutants are able to utilize arbutin, salicin, and, to a lesser extent, cellobiose as sole carbon and energy sources [Parker88]. ascG deletion strains have a positive response to arbutin and salicin in Biolog phenotype microarrays [Ishida09]. AscG is maintained at a high level under ordinary culture conditions [Ishida09].

AscF and AscB are paralogues of BglF and BglB, respectively [Hall92a]. The bgl and asc operons are estimated to have arisen by operon duplication about 3x108 years ago [Hall92a].

asc: arbutin, salicin, cellobiose

Citations: [Hall91]

Locations: inner membrane

Map Position: [2,837,546 -> 2,839,003] (61.16 centisomes)
Length: 1458 bp / 485 aa

Molecular Weight of Polypeptide: 51.026 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0008926 , CGSC:33233 , EchoBASE:EB0084 , EcoGene:EG10086 , EcoliWiki:b2715 , ModBase:P24241 , OU-Microarray:b2715 , PortEco:ascF , PR:PRO_000022162 , Pride:P24241 , Protein Model Portal:P24241 , RefSeq:YP_026182 , RegulonDB:EG10086 , SMR:P24241 , String:511145.b2715 , UniProt:P24241

Relationship Links: InterPro:IN-FAMILY:IPR001996 , InterPro:IN-FAMILY:IPR003352 , InterPro:IN-FAMILY:IPR004719 , InterPro:IN-FAMILY:IPR013013 , InterPro:IN-FAMILY:IPR018113 , Pfam:IN-FAMILY:PF00367 , Pfam:IN-FAMILY:PF02378 , Prosite:IN-FAMILY:PS01035 , Prosite:IN-FAMILY:PS51098 , Prosite:IN-FAMILY:PS51103

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
GO:0008643 - carbohydrate transport Inferred by computational analysis [UniProtGOA11]
GO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0016310 - phosphorylation Inferred by computational analysis [UniProtGOA11]
GO:0034219 - carbohydrate transmembrane transport Inferred by computational analysis [GOA01a, GOA01]
Molecular Function: GO:0008982 - protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred by computational analysis [GOA01a, GOA01]
GO:0016301 - kinase activity Inferred by computational analysis [UniProtGOA11]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, DiazMejia09, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11, GOA01]

MultiFun Terms: All-Genes Pseudo-Genes Cryptic-Genes
MultiFun cell structure membrane
MultiFun metabolism carbon utilization carbon compounds
MultiFun transport Group Translocators Phosphotransferase Systems (PEP-dependent PTS)

Essentiality data for ascF knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Credits:
Last-Curated ? 27-Mar-2014 by Mackie A , Macquarie University


Enzymatic reaction of: transport of cellobiose (β-glucoside PTS permease AscF (cryptic))

Note: The enzyme may catalyze this reaction in vitro, but this reaction is not considered to be physiologically relevant.


Enzymatic reaction of: transport of salicin (β-glucoside PTS permease AscF (cryptic))

Note: The enzyme may catalyze this reaction in vitro, but this reaction is not considered to be physiologically relevant.


Enzymatic reaction of: transport of arbutin (β-glucoside PTS permease AscF (cryptic))

Note: The enzyme may catalyze this reaction in vitro, but this reaction is not considered to be physiologically relevant.


Sequence Features

Feature Class Location Citations Comment
Conserved-Region 1 -> 88
[UniProt09]
UniProt: PTS EIIB type-1;
Active-Site 28
[UniProt10]
UniProt: Phosphocysteine intermediate; for EIIB activity; Non-Experimental Qualifier: by similarity;
Transmembrane-Region 102 -> 122
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Conserved-Region 108 -> 470
[UniProt09]
UniProt: PTS EIIC type-1;
Transmembrane-Region 147 -> 167
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Sequence-Conflict 167
[Hall92a, UniProt10a]
[Blattner97, UniProt10a]
Alternate sequence: A → Q; UniProt: (in Ref. 1; AAA16429);
Alternate sequence: ASAA → HLPR; UniProt: (in Ref. 2; AAA69225);
Transmembrane-Region 177 -> 197
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 207 -> 227
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 254 -> 274
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 285 -> 305
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Sequence-Conflict 311
[Hall92a, UniProt10a]
Alternate sequence: R → H; UniProt: (in Ref. 1; AAA16429);
Transmembrane-Region 330 -> 350
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 363 -> 383
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 389 -> 409
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 433 -> 453
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b2715 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10086; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Blattner97: Blattner FR, Plunkett G, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y (1997). "The complete genome sequence of Escherichia coli K-12." Science 277(5331);1453-74. PMID: 9278503

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA01a: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

Hall91: Hall BG, Xu L, Ochman H (1991). "Physical map location of the asc (formerly sac) operon of Escherichia coli K-12." J Bacteriol 173(17);5250. PMID: 1885507

Hall92a: Hall BG, Xu L (1992). "Nucleotide sequence, function, activation, and evolution of the cryptic asc operon of Escherichia coli K12." Mol Biol Evol 9(4);688-706. PMID: 1630307

Ishida09: Ishida Y, Kori A, Ishihama A (2009). "Participation of regulator AscG of the beta-glucoside utilization operon in regulation of the propionate catabolism operon." J Bacteriol 191(19);6136-44. PMID: 19633077

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Parker88: Parker LL, Hall BG (1988). "A fourth Escherichia coli gene system with the potential to evolve beta-glucoside utilization." Genetics 119(3);485-90. PMID: 3042507

Postma93: Postma PW, Lengeler JW, Jacobson GR (1993). "Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria." Microbiol Rev 57(3);543-94. PMID: 8246840

Saier14: Saier MH, Reddy VS, Tamang DG, Vastermark A (2014). "The transporter classification database." Nucleic Acids Res 42(1);D251-8. PMID: 24225317

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Beisel12: Beisel CL, Updegrove TB, Janson BJ, Storz G (2012). "Multiple factors dictate target selection by Hfq-binding small RNAs." EMBO J 31(8);1961-74. PMID: 22388518


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Nov 21, 2014, BIOCYC14B.