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BioCyc websites down
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Escherichia coli K-12 substr. MG1655 Polypeptide: MalI DNA-binding transcriptional repressor



Gene: malI Accession Numbers: EG10557 (EcoCyc), b1620, ECK1615

Regulation Summary Diagram: ?

Summary:
"Maltose inhibitor," MalI, is a repressor that controls genes related to the maltose system. It is negatively autoregulated and coordinately represses transcription of a divergent operon that encodes a maltose-glucose PTS permease and a bifunctional protein that interacts directly with the MalT transcriptional activator [Reidl89, Reidl91].

This transcription factor binds to two 12-bp-long direct repeat nucleotide sequences in tandem, overlapping the malXp and malIp simultaneously [Reidl89, Reidl91].

MalI shows high similarity to GalR (28%), CytR (21%), and LacI (24%), and homology to a fragment of 30 amino acids from MalT. Additionally, the N-terminal region has a helix-turn-helix DNA-binding domain [Reidl89]. The gene encoding this protein is homologous to transcriptional repressors of the LacI/GalR family.

Locations: cytosol

Map Position: [1,696,176 <- 1,697,204] (36.56 centisomes)
Length: 1029 bp / 342 aa

Molecular Weight of Polypeptide: 36.625 kD (from nucleotide sequence)

pI: 6.77

Unification Links: ASAP:ABE-0005421 , CGSC:18181 , DIP:DIP-10143N , EchoBASE:EB0552 , EcoGene:EG10557 , EcoliWiki:b1620 , ModBase:P18811 , OU-Microarray:b1620 , PortEco:malI , PR:PRO_000023150 , Protein Model Portal:P18811 , RefSeq:NP_416137 , RegulonDB:EG10557 , SMR:P18811 , String:511145.b1620 , UniProt:P18811

Relationship Links: InterPro:IN-FAMILY:IPR000843 , InterPro:IN-FAMILY:IPR001761 , InterPro:IN-FAMILY:IPR010982 , InterPro:IN-FAMILY:IPR026909 , InterPro:IN-FAMILY:IPR028082 , Panther:IN-FAMILY:PTHR30146:SF3 , Panther:IN-FAMILY:PTHR30146:SF7 , Pfam:IN-FAMILY:PF00356 , Pfam:IN-FAMILY:PF00532 , Prosite:IN-FAMILY:PS00356 , Prosite:IN-FAMILY:PS50932 , Smart:IN-FAMILY:SM00354

In Paralogous Gene Group: 30 (25 members)

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0045892 - negative regulation of transcription, DNA-templated Inferred from experiment [Reidl89]
GO:0006351 - transcription, DNA-templated Inferred by computational analysis [UniProtGOA11]
GO:0006355 - regulation of transcription, DNA-templated Inferred by computational analysis [UniProtGOA11, GOA01]
Molecular Function: GO:0003677 - DNA binding Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0003700 - sequence-specific DNA binding transcription factor activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: information transfer RNA related Transcription related
metabolism carbon utilization carbon compounds
regulation genetic unit regulated operon
regulation type of regulation transcriptional level repressor

DNA binding site length: 18 base-pairs

Symmetry: Inverted Repeat

Consensus DNA Binding Sequence: aGaTAAAACGTTTTATCT

Regulated Transcription Units (2 total): ?

Notes:

Essentiality data for malI knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Sequence Features

Feature Class Location Citations Comment
Conserved-Region 7 -> 61
[UniProt09]
UniProt: HTH lacI-type;
DNA-Binding-Region 9 -> 28
[UniProt10a]
UniProt: H-T-H motif; Non-Experimental Qualifier: by similarity;
Sequence-Conflict 287 -> 297
[Reidl89, Reidl91, UniProt10]
Alternate sequence: FTDATPTTLDD → IYRCDTNHTCMH; UniProt: (in Ref. 1 and 2);
Sequence-Conflict 317 -> 342
[Reidl89, Reidl91, UniProt10]
Alternate sequence: RMMQKITHEETHSRNLIIPARLIAAK → SHDAKNHP; UniProt: (in Ref. 1 and 2);


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
10/20/97 Gene b1620 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10557; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Reidl89: Reidl J, Romisch K, Ehrmann M, Boos W (1989). "MalI, a novel protein involved in regulation of the maltose system of Escherichia coli, is highly homologous to the repressor proteins GalR, CytR, and LacI." J Bacteriol 1989;171(9);4888-99. PMID: 2670898

Reidl91: Reidl J, Boos W (1991). "The malX malY operon of Escherichia coli encodes a novel enzyme II of the phosphotransferase system recognizing glucose and maltose and an enzyme abolishing the endogenous induction of the maltose system." J Bacteriol 173(15);4862-76. PMID: 1856179

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Lloyd08: Lloyd GS, Hollands K, Godfrey RE, Busby SJ (2008). "Transcription initiation in the Escherichia coli K-12 malI-malX intergenic region and the role of the cyclic AMP receptor protein." FEMS Microbiol Lett 288(2);250-7. PMID: 19054084

Lloyd10: Lloyd GS, Godfrey RE, Busby SJ (2010). "Targets for the MalI repressor at the divergent Escherichia coli K-12 malX-malI promoters." FEMS Microbiol Lett 305(1);28-34. PMID: 20141531

Otsuka96: Otsuka J, Watanabe H, Mori KT (1996). "Evolution of transcriptional regulation system through promiscuous coupling of regulatory proteins with operons; suggestion from protein sequence similarities in Escherichia coli." J Theor Biol 1996;178(2);183-204. PMID: 8729576

Zheng04: Zheng D, Constantinidou C, Hobman JL, Minchin SD (2004). "Identification of the CRP regulon using in vitro and in vivo transcriptional profiling." Nucleic Acids Res 32(19);5874-93. PMID: 15520470


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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