Escherichia coli K-12 substr. MG1655 Polypeptide: protease involved in Microcin B17 maturation and in sensitivity to the DNA gyrase inhibitor LetD

Gene: pmbA Accession Numbers: EG10741 (EcoCyc), b4235, ECK4230

Synonyms: mcb, tldE

Regulation Summary Diagram: ?

Regulation summary diagram for pmbA

TldD and/or TldE act as a protease [Allali02] that is involved in sensitivity to DNA gyrase inhibitor LetD/CcdB (encoded by the F plasmid) [Murayama96] by effecting degradation of the F plasmid-encoded LetD inhibitor, LetA/CcdA [Allali02]. TldD [Allali02] and TldE [RodriguezSainz90, Allali02] are also involved in proteolytic processing of the antibiotic Microcin B17 (encoded by the pMccB17 plasmid) prior to its export from the cell. TldD, TldE and GroE have been proposed to antagonize ZfiA-mediated inhibition of the binding of the F-plasmid-encoded LetD DNA gyrase inhibitor to the DNA gyrase A subunit [Murayama96].

A tldD [Allali02] or tldE [RodriguezSainz90, Allali02] mutant harboring the pMccB17 episome exhibits accumulation of Microcin B17 precursor protein, in contrast to wild type, which exhibits processing and the export of Microcin B17 from the cell. A tldD, tldE, or groES mutant exhibits resistance to the F-plasmid-encoded LetD DNA gyrase inhibitor [Murayama96]. A zfiA mutation suppresses the resistance to the F-plasmid-encoded LetD DNA gyrase inhibitor exhibited by a tldD, tldE or groES mutant [Murayama96].

TldE is cytoplasmic [RodriguezSainz90].

Locations: cytosol

Map Position: [4,455,982 -> 4,457,334] (96.04 centisomes, 346°)
Length: 1353 bp / 450 aa

Molecular Weight of Polypeptide: 48.37 kD (from nucleotide sequence), 48.4 kD (experimental) [RodriguezSainz90 ]

Unification Links: ASAP:ABE-0013854 , CGSC:34577 , DIP:DIP-47844N , EchoBASE:EB0734 , EcoGene:EG10741 , EcoliWiki:b4235 , OU-Microarray:b4235 , PortEco:pmbA , PR:PRO_000023558 , Pride:P0AFK0 , Protein Model Portal:P0AFK0 , RefSeq:NP_418656 , RegulonDB:EG10741 , SMR:P0AFK0 , String:511145.b4235 , Swiss-Model:P0AFK0 , UniProt:P0AFK0

Relationship Links: InterPro:IN-FAMILY:IPR002510 , Pfam:IN-FAMILY:PF01523

In Paralogous Gene Group: 507 (2 members)

GO Terms:

Biological Process: GO:0006508 - proteolysis Inferred from experiment Inferred by computational analysis [UniProtGOA11, Allali02]
Molecular Function: GO:0008233 - peptidase activity Inferred by computational analysis [UniProtGOA11]
GO:0008237 - metallopeptidase activity Inferred by computational analysis [UniProtGOA11]
GO:0016787 - hydrolase activity Inferred by computational analysis [UniProtGOA11]
Cellular Component: GO:0005737 - cytoplasm Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, RodriguezSainz90]
GO:0005829 - cytosol Inferred from experiment Inferred by computational analysis [DiazMejia09, Ishihama08, LopezCampistrou05]

MultiFun Terms: cell processes protection cell killing
information transfer protein related chaperoning, repair (refolding)

Essentiality data for pmbA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


10/20/97 Gene b4235 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10741; confirmed by SwissProt match.


Allali02: Allali N, Afif H, Couturier M, Van Melderen L (2002). "The highly conserved TldD and TldE proteins of Escherichia coli are involved in microcin B17 processing and in CcdA degradation." J Bacteriol 2002;184(12);3224-31. PMID: 12029038

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

Ishihama08: Ishihama Y, Schmidt T, Rappsilber J, Mann M, Hartl FU, Kerner MJ, Frishman D (2008). "Protein abundance profiling of the Escherichia coli cytosol." BMC Genomics 9;102. PMID: 18304323

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

LopezCampistrou05: Lopez-Campistrous A, Semchuk P, Burke L, Palmer-Stone T, Brokx SJ, Broderick G, Bottorff D, Bolch S, Weiner JH, Ellison MJ (2005). "Localization, annotation, and comparison of the Escherichia coli K-12 proteome under two states of growth." Mol Cell Proteomics 4(8);1205-9. PMID: 15911532

Murayama96: Murayama N, Shimizu H, Takiguchi S, Baba Y, Amino H, Horiuchi T, Sekimizu K, Miki T (1996). "Evidence for involvement of Escherichia coli genes pmbA, csrA and a previously unrecognized gene tldD, in the control of DNA gyrase by letD (ccdB) of sex factor F." J Mol Biol 1996;256(3);483-502. PMID: 8604133

RodriguezSainz90: Rodriguez-Sainz MC, Hernandez-Chico C, Moreno F (1990). "Molecular characterization of pmbA, an Escherichia coli chromosomal gene required for the production of the antibiotic peptide MccB17." Mol Microbiol 1990;4(11);1921-32. PMID: 2082149

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

MendozaVargas09: Mendoza-Vargas A, Olvera L, Olvera M, Grande R, Vega-Alvarado L, Taboada B, Jimenez-Jacinto V, Salgado H, Juarez K, Contreras-Moreira B, Huerta AM, Collado-Vides J, Morett E (2009). "Genome-wide identification of transcription start sites, promoters and transcription factor binding sites in E. coli." PLoS One 4(10);e7526. PMID: 19838305

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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