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Escherichia coli K-12 substr. MG1655 Polypeptide: stimulates PspC-mediated transcriptional activation of the psp operon; antitoxin of a PspC-PspB toxin-antitoxin pair



Gene: pspB Accession Numbers: EG10777 (EcoCyc), b1305, ECK1300

Synonyms: phage shock protein

Regulation Summary Diagram: ?

Summary:
PspB and PspC together act to activate transcription of the psp operon induced by infection with bacteriophage, exposure to ethanol, or osmotic shock (but not heat treatment); PspC is essential for this activation, whereas PspB is not strictly required [Weiner91]. PspC and PspB are also reported to be a toxin-antitoxin pair [Brown03].

PspA, PspB, and PspC form a complex, and PspC is required for PspA to bind to PspB [Adams03]. PspA, PspB, and PspC are not observed to cross-link with PspD [Adams03].

A pspB mutant exhibits a defect in protein translocation that is suppressed by production of PspA, which plays a role in maintenance of the protonmotive force under certain conditions of cellular stress [Kleerebezem96a]. Multi-copy overexpression of the psp operon increases survival upon stress caused by n-hexane treatment [Kobayashi98a].

The corresponding psp locus of Yersinia enterocolitica is virulence-related [Darwin01].

Regulation has been described [Brissette91, Weiner91, Dworkin97, Dworkin98, Jovanovic99, Carmona99, Kobayashi98a, Weiner91, Jovanovic96, Weiner95, Weiner94, Bergler94a]. The psp operon shows induction upon phage infection, temperature increase, or exposure to ethanol, osmotic shock [Brissette91], or the organic solvents n-hexane or cyclooctane [Kobayashi98a]. Induction is mediated by sigma54, PspB, PspC [Weiner91], PspF [Jovanovic96], and IHF [Weiner95]. Transcription is induced by conditions that cause stress related to energy depletion [Weiner94]. Treatment with the drugs diazaborine or cerulenin, which inhibit synthesis of fatty acids and phospholipids, or treatment with globomycin, which disrupts lipoprotein processing, causes transcriptional induction of pspA [Bergler94a]. Transcription is repressed by PspA and by the heat shock (sigma32-dependent) system [Weiner91].

PspB: phage shock protein [Brissette91].

Review: [Model97, Darwin05].

Gene Citations: [Fozo08]

Locations: inner membrane

Map Position: [1,366,825 -> 1,367,049] (29.46 centisomes)
Length: 225 bp / 74 aa

Molecular Weight of Polypeptide: 8.763 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0004389 , CGSC:32012 , EchoBASE:EB0770 , EcoGene:EG10777 , EcoliWiki:b1305 , OU-Microarray:b1305 , PortEco:pspB , PR:PRO_000023615 , Protein Model Portal:P0AFM9 , RefSeq:NP_415821 , RegulonDB:EG10777 , String:511145.b1305 , UniProt:P0AFM9

Relationship Links: InterPro:IN-FAMILY:IPR009554 , Pfam:IN-FAMILY:PF06667

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0006351 - transcription, DNA-templated Inferred from experiment Author statement [Brown03, Weiner91]
GO:0006355 - regulation of transcription, DNA-templated Inferred by computational analysis [GOA01a]
GO:0006950 - response to stress Inferred by computational analysis [UniProtGOA11]
GO:0009271 - phage shock Inferred by computational analysis [GOA01a]
Cellular Component: GO:0005886 - plasma membrane Inferred by computational analysis [UniProtGOA11a, UniProtGOA11]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11]

MultiFun Terms: extrachromosomal prophage genes and phage related functions
information transfer RNA related Transcription related
regulation genetic unit regulated operon
regulation type of regulation transcriptional level activator

Essentiality data for pspB knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Sequence Features

Feature Class Location Citations Comment
Transmembrane-Region 4 -> 24
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
10/20/97 Gene b1305 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10777; confirmed by SwissProt match.


References

Adams03: Adams H, Teertstra W, Demmers J, Boesten R, Tommassen J (2003). "Interactions between phage-shock proteins in Escherichia coli." J Bacteriol 2003;185(4);1174-80. PMID: 12562786

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Bergler94a: Bergler H, Abraham D, Aschauer H, Turnowsky F (1994). "Inhibition of lipid biosynthesis induces the expression of the pspA gene." Microbiology 140 ( Pt 8);1937-44. PMID: 7921245

Brissette91: Brissette JL, Weiner L, Ripmaster TL, Model P (1991). "Characterization and sequence of the Escherichia coli stress-induced psp operon." J Mol Biol 220(1);35-48. PMID: 1712397

Brown03: Brown JM, Shaw KJ (2003). "A novel family of Escherichia coli toxin-antitoxin gene pairs." J Bacteriol 185(22);6600-8. PMID: 14594833

Carmona99: Carmona M, de Lorenzo V (1999). "Involvement of the FtsH (HflB) protease in the activity of sigma 54 promoters." Mol Microbiol 31(1);261-70. PMID: 9987127

Darwin01: Darwin AJ, Miller VL (2001). "The psp locus of Yersinia enterocolitica is required for virulence and for growth in vitro when the Ysc type III secretion system is produced." Mol Microbiol 39(2);429-44. PMID: 11136463

Darwin05: Darwin AJ (2005). "The phage-shock-protein response." Mol Microbiol 57(3);621-8. PMID: 16045608

Dworkin97: Dworkin J, Jovanovic G, Model P (1997). "Role of upstream activation sequences and integration host factor in transcriptional activation by the constitutively active prokaryotic enhancer-binding protein PspF." J Mol Biol 273(2);377-88. PMID: 9344746

Dworkin98: Dworkin J, Ninfa AJ, Model P (1998). "A protein-induced DNA bend increases the specificity of a prokaryotic enhancer-binding protein." Genes Dev 12(6);894-900. PMID: 9512522

Fozo08: Fozo EM, Kawano M, Fontaine F, Kaya Y, Mendieta KS, Jones KL, Ocampo A, Rudd KE, Storz G (2008). "Repression of small toxic protein synthesis by the Sib and OhsC small RNAs." Mol Microbiol 70(5):1076-93. PMID: 18710431

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Jovanovic96: Jovanovic G, Weiner L, Model P (1996). "Identification, nucleotide sequence, and characterization of PspF, the transcriptional activator of the Escherichia coli stress-induced psp operon." J Bacteriol 178(7);1936-45. PMID: 8606168

Jovanovic99: Jovanovic G, Rakonjac J, Model P (1999). "In vivo and in vitro activities of the Escherichia coli sigma54 transcription activator, PspF, and its DNA-binding mutant, PspFDeltaHTH." J Mol Biol 285(2);469-83. PMID: 9878422

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Kleerebezem96a: Kleerebezem M, Crielaard W, Tommassen J (1996). "Involvement of stress protein PspA (phage shock protein A) of Escherichia coli in maintenance of the protonmotive force under stress conditions." EMBO J 1996;15(1);162-71. PMID: 8598199

Kobayashi98a: Kobayashi H, Yamamoto M, Aono R (1998). "Appearance of a stress-response protein, phage-shock protein A, in Escherichia coli exposed to hydrophobic organic solvents." Microbiology 144 ( Pt 2);353-9. PMID: 9493373

Model97: Model P, Jovanovic G, Dworkin J (1997). "The Escherichia coli phage-shock-protein (psp) operon." Mol Microbiol 24(2);255-61. PMID: 9159513

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Weiner91: Weiner L, Brissette JL, Model P (1991). "Stress-induced expression of the Escherichia coli phage shock protein operon is dependent on sigma 54 and modulated by positive and negative feedback mechanisms." Genes Dev 5(10);1912-23. PMID: 1717346

Weiner94: Weiner L, Model P (1994). "Role of an Escherichia coli stress-response operon in stationary-phase survival." Proc Natl Acad Sci U S A 91(6);2191-5. PMID: 8134371

Weiner95: Weiner L, Brissette JL, Ramani N, Model P (1995). "Analysis of the proteins and cis-acting elements regulating the stress-induced phage shock protein operon." Nucleic Acids Res 23(11);2030-6. PMID: 7596833

Other References Related to Gene Regulation

Hou12: Hou Z, Fink RC, Black EP, Sugawara M, Zhang Z, Diez-Gonzalez F, Sadowsky MJ (2012). "Gene expression profiling of Escherichia coli in response to interactions with the lettuce rhizosphere." J Appl Microbiol 113(5);1076-86. PMID: 22830299

Huvet11: Huvet M, Toni T, Sheng X, Thorne T, Jovanovic G, Engl C, Buck M, Pinney JW, Stumpf MP (2011). "The evolution of the phage shock protein response system: interplay between protein function, genomic organization, and system function." Mol Biol Evol 28(3);1141-55. PMID: 21059793

Jovanovic97: Jovanovic G, Dworkin J, Model P (1997). "Autogenous control of PspF, a constitutively active enhancer-binding protein of Escherichia coli." J Bacteriol 1997;179(16);5232-7. PMID: 9260970

Jovanovic97a: Jovanovic G, Model P (1997). "PspF and IHF bind co-operatively in the psp promoter-regulatory region of Escherichia coli." Mol Microbiol 1997;25(3);473-81. PMID: 9302010

Kamenšek13: Kamenšek S, Žgur-Bertok D (2013). "Global transcriptional responses to the bacteriocin colicin M in Escherichia coli." BMC Microbiol 13;42. PMID: 23421615

Zaslaver06: Zaslaver A, Bren A, Ronen M, Itzkovitz S, Kikoin I, Shavit S, Liebermeister W, Surette MG, Alon U (2006). "A comprehensive library of fluorescent transcriptional reporters for Escherichia coli." Nat Methods 3(8);623-8. PMID: 16862137


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sat Dec 20, 2014, BIOCYC14A.