|Gene:||recE||Accession Numbers: EG10827 (EcoCyc), b1350, ECK1347|
Synonyms: rmuB, rac, sbcA
DNA damage can occur due to a variety of environmental assaults including UV irradiation and chemical agents. Escherichia coli has a number of complex enzymatic pathways for the repair of DNA damage. Most DNA damage involves lesions to one strand of DNA, but in some cases damage can occur to both strands. Double strand breaks (DSBs) cannot be repaired via excision repair pathways because by their nature they lack an intact strand to be used as a template. Repair of DSBs utilize the RecA pathway of homologous recombination with a separate, intact, homologous region of DNA.
The RecE (also known as exonuclease VIII) pathway is an alternative pathway for the initiation of homologous recombination in Escherichia coli. The RecE pathway is activated in recB, recC sbcA (suppressors of recB and recC) mutants. Activation of the RecE pathway results in the production of at least two phage proteins relevant for recombinational repair. RecE is a duplex DNA-specific exonuclease which produces 3' overhangs in duplex DNA by preferentially degrading the 5'-ending strands [Kuzminov99, Joseph83]. RecT promotes the annealing of complementary single strands and can also catalyze three-strand recombinational junction branch migration [Kuzminov99].
The crystal structure of the C-terminal nuclease domain of RecE has been resolved at 2.8Å resolution [Zhang09c]. The RecE C-terminal domain comprising amino acid residues 606-866 forms a stable tetramer in solution [Zhang09c].
recE transcription is increased specifically in response to 2,4,6-trinitrotoluene and its indicator compounds 1,3-DNB, 2,4-DNT, and 2,6-DNT; the response was utilized for construction of an E. coli biosensor strain [Tan15].
|Map Position: [1,412,810 <- 1,415,410] (30.45 centisomes, 110°)||Length: 2601 bp / 866 aa|
Molecular Weight of Polypeptide: 96.368 kD (from nucleotide sequence)
Unification Links: ASAP:ABE-0004531 , CGSC:309 , DIP:DIP-10652N , EchoBASE:EB0820 , EcoGene:EG10827 , EcoliWiki:b1350 , Mint:MINT-1262359 , OU-Microarray:b1350 , PortEco:recE , PR:PRO_000023700 , Pride:P15032 , Protein Model Portal:P15032 , RefSeq:NP_415866 , RegulonDB:EG10827 , SMR:P15032 , String:511145.b1350 , UniProt:P15032
|Biological Process:||GO:0000738 - DNA catabolic process, exonucleolytic
GO:0008152 - metabolic process [UniProtGOA11a]
GO:0090305 - nucleic acid phosphodiester bond hydrolysis [UniProtGOA11a]
|Molecular Function:||GO:0051908 - double-stranded DNA 5'-3' exodeoxyribonuclease activity
GO:0004518 - nuclease activity [UniProtGOA11a]
GO:0004527 - exonuclease activity [UniProtGOA11a]
GO:0016787 - hydrolase activity [UniProtGOA11a]
|MultiFun Terms:||extrachromosomal → prophage genes and phage related functions|
|information transfer → DNA related → DNA degradation|
|information transfer → DNA related → DNA repair|
|metabolism → degradation of macromolecules → DNA|
|Growth Medium||Growth?||T (°C)||O2||pH||Osm/L||Growth Observations|
|LB enriched||Yes||37||Aerobic||6.95||Yes [Gerdes03, Comment 1]|
|LB Lennox||Yes||37||Aerobic||7||Yes [Baba06, Comment 2]|
|M9 medium with 1% glycerol||Yes||37||Aerobic||7.2||0.35||Yes [Joyce06, Comment 3]|
|MOPS medium with 0.4% glucose||Yes||37||Aerobic||7.2||0.22||Yes [Baba06, Comment 2]|
Enzymatic reaction of: DNA exonuclease
EC Number: 3.1.11.-
The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.
The reaction is physiologically favored in the direction shown.
|Sequence-Conflict||691 -> 692|
10/20/97 Gene b1350 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10827; confirmed by SwissProt match.
Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554
Clark93: Clark AJ, Sharma V, Brenowitz S, Chu CC, Sandler S, Satin L, Templin A, Berger I, Cohen A (1993). "Genetic and molecular analyses of the C-terminal region of the recE gene from the Rac prophage of Escherichia coli K-12 reveal the recT gene." J Bacteriol 175(23);7673-82. PMID: 8244937
Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938
Ishioka97: Ishioka K, Iwasaki H, Shinagawa H (1997). "Roles of the recG gene product of Escherichia coli in recombination repair: effects of the delta recG mutation on cell division and chromosome partition." Genes Genet Syst 72(2);91-9. PMID: 9265736
Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394
Tan15: Tan J, Kan N, Wang W, Ling J, Qu G, Jin J, Shao Y, Liu G, Chen H (2015). "Construction of 2,4,6-Trinitrotoluene Biosensors with Novel Sensing Elements from Escherichia coli K-12 MG1655." Cell Biochem Biophys. PMID: 25561288
Zhang09c: Zhang J, Xing X, Herr AB, Bell CE (2009). "Crystal structure of E. coli RecE protein reveals a toroidal tetramer for processing double-stranded DNA breaks." Structure 17(5);690-702. PMID: 19446525
Guo14: Guo Y, Quiroga C, Chen Q, McAnulty MJ, Benedik MJ, Wood TK, Wang X (2014). "RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli." Nucleic Acids Res 42(10);6448-62. PMID: 24748661
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