Escherichia coli K-12 substr. MG1655 Enzyme: RecJ

Gene: recJ Accession Numbers: EG10830 (EcoCyc), b2892, ECK2887

Regulation Summary Diagram: ?

Regulation summary diagram for recJ

Three essential activities for the initiation of recombination in the recB1080 mutant are provided by different proteins: helicase activity by RecB1080CD, 5' --> 3' exonuclease by RecJ, and RecA-single-stranded DNA filament formation by RecFOR [IvancicBace03]

Citations: [Handa09, Morimatsu14]

Gene Citations: [Nonaka06, Rhodius05]

Locations: cytosol

Map Position: [3,034,395 <- 3,036,128] (65.4 centisomes, 235°)
Length: 1734 bp / 577 aa

Molecular Weight of Polypeptide: 63.389 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0009492 , CGSC:17965 , DIP:DIP-10654N , EchoBASE:EB0823 , EcoGene:EG10830 , EcoliWiki:b2892 , Mint:MINT-1223840 , ModBase:P21893 , OU-Microarray:b2892 , PortEco:recJ , PR:PRO_000023703 , Pride:P21893 , Protein Model Portal:P21893 , RefSeq:NP_417368 , RegulonDB:EG10830 , SMR:P21893 , String:511145.b2892 , UniProt:P21893

Relationship Links: InterPro:IN-FAMILY:IPR001667 , InterPro:IN-FAMILY:IPR003156 , InterPro:IN-FAMILY:IPR004610 , Pfam:IN-FAMILY:PF01368 , Pfam:IN-FAMILY:PF02272

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Genetic Regulation Schematic: ?

Genetic regulation schematic for recJ

GO Terms:

Biological Process: GO:0006281 - DNA repair Inferred by computational analysis [GOA01]
GO:0006310 - DNA recombination Inferred by computational analysis [GOA01]
GO:0090305 - nucleic acid phosphodiester bond hydrolysis Inferred by computational analysis [UniProtGOA11, GOA01]
Molecular Function: GO:0003676 - nucleic acid binding Inferred by computational analysis [GOA01]
GO:0004518 - nuclease activity Inferred by computational analysis [UniProtGOA11]
GO:0004527 - exonuclease activity Inferred by computational analysis [UniProtGOA11]
GO:0008409 - 5'-3' exonuclease activity Inferred by computational analysis [GOA01]
GO:0016787 - hydrolase activity Inferred by computational analysis [UniProtGOA11]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: information transfer DNA related DNA degradation
metabolism degradation of macromolecules DNA

Essentiality data for recJ knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Enzymatic reaction of: RecJ exonuclease

EC Number: 3.1.-.-

DNA with lesion <=> DNA with lesion resolved through Rec-mediated pathway

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Reversibility of this reaction is unspecified.

Sequence Features

Protein sequence of RecJ with features indicated

Feature Class Location Citations Comment
Sequence-Conflict 6
[Lovett91, UniProt10]
UniProt: (in Ref. 1; AAA62789);
Sequence-Conflict 20
[Lovett91, UniProt10]
UniProt: (in Ref. 1; AAA62789);

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Units:

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram


10/20/97 Gene b2892 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10830; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Handa09: Handa N, Morimatsu K, Lovett ST, Kowalczykowski SC (2009). "Reconstitution of initial steps of dsDNA break repair by the RecF pathway of E. coli." Genes Dev 23(10);1234-45. PMID: 19451222

IvancicBace03: Ivancic-Bace I, Peharec P, Moslavac S, Skrobot N, Salaj-Smic E, Brcic-Kostic K (2003). "RecFOR function is required for DNA repair and recombination in a RecA loading-deficient recB mutant of Escherichia coli." Genetics 163(2);485-94. PMID: 12618388

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Lovett91: Lovett ST, Kolodner RD (1991). "Nucleotide sequence of the Escherichia coli recJ chromosomal region and construction of recJ-overexpression plasmids." J Bacteriol 173(1);353-64. PMID: 1987126

Morimatsu14: Morimatsu K, Kowalczykowski SC (2014). "RecQ helicase and RecJ nuclease provide complementary functions to resect DNA for homologous recombination." Proc Natl Acad Sci U S A 111(48);E5133-42. PMID: 25411316

Nonaka06: Nonaka G, Blankschien M, Herman C, Gross CA, Rhodius VA (2006). "Regulon and promoter analysis of the E. coli heat-shock factor, sigma32, reveals a multifaceted cellular response to heat stress." Genes Dev 20(13);1776-89. PMID: 16818608

Rhodius05: Rhodius VA, Suh WC, Nonaka G, West J, Gross CA (2005). "Conserved and variable functions of the sigmaE stress response in related genomes." PLoS Biol 4(1);e2. PMID: 16336047

UniProt10: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Fri Oct 9, 2015, biocyc13.