Escherichia coli K-12 substr. MG1655 Polypeptide: RNase P protein component; processes tRNA, 4.5S RNA

Gene: rnpA Accession Numbers: EG10862 (EcoCyc), b3704, ECK3696

Regulation Summary Diagram: ?

Regulation summary diagram for rnpA

Component of: RNase P

RnpB RNA and RnpA protein together compose ribonuclease P (RNAse P) [Kole79, Kole81]. RNAse P acts in processing of 4.5S RNA [Bothwell76] and tRNA precursor molecules [Schedl75]. The RnpB RNA subunit alone exhibits catalytic activity toward tRNA precursors, but requires the protein subunit for efficient activity toward an 4.5S RNA precursor substrate [GuerrierTakada83, GuerrierTakada84, PeckMiller91]. RNAse P also exhibits activity toward C4 repressor RNA of bacteriophages P1 and P7, which may indicate a role for RNAse P in lysogeny/lysis regulation [Hartmann95], and toward CI RNA, which is involved in immunity to bacteriophage P4 [Briani02].

Activity of the RNAse P RNA and holoenzyme [Schedl75, Chang75, Bikoff75, Bikoff75a, Sakano75, Kirsebom89, Ilgen76, Burgin90, GuerrierTakada83, GuerrierTakada84, Orellana86, GuerrierTakada86, GuerrierTakada88, Vioque89, Surratt90, PeckMiller91, Kazakov91, Nomura88, Tallsjo93, Hardt95, Kufel94, Hartmann95, Steitz93, Warnecke96, Lazard98, Brannvall98, Hori01, Li02, Kirsebom92, Mikkelsen99, Warnecke00, Pomeranz00, Busch00, Tanaka01, Eubank02, Kaye02, Persson03] and substrate interaction and specificity [Schmidt76, Bothwell76a, Bothwell76a, Thurlow91, Subbarao84, Shlyapnikov86, Kirsebom89, GuerrierTakada84a, Holm92, Green88a, McClain87, Burkard88, Reilly86, Baer88, Vold88, Burkard88a, GuerrierTakada88, Samuelsson88, GuerrierTakada89, Knap90, Surratt90a, Surratt90, Krupp91, Perreault92, Svard92, Schlegl92, Furdon83, Gurevitz83, Nolan93, Gaur93, Svard93, Kleineidam93, Kirsebom93, GuerrierTakada93, Hardt93, Hardt93a, Westhof94, LaGrandeur94, Oh94, Kirsebom94, Hardt95a, Meinnel95a, Yan94, Tallsjo96, Svard96, Kufel96, Gaur96, Liu96a, Kufel96a, Lazard98, Brannvall98, Park00, Brannvall02, Brannvall03, Hardt96, Harris97, Christian98, Heide99, Siew99, Pascual99, Heide01, Heide01a, Hansen01b, Zahler03, Ando03, Ando03a, Tanaka03, Nagai03, Ando03b] have been examined in detail.

The association of RnpB RNA and RnpA protein has been examined [Vioque88, Baer89, Talbot94, Talbot94a, Gopalan97, Gopalan99, Kim00a, Biswas00, Sharkady01, Tsai03]. The enzyme complex exhibits 1:1 stoichiometry of RnpB RNA to RnpA protein [Talbot94].

RNAse P fractionates with the inner membrane [Srivastava91]. Overproduced RNAse P enzyme is also localized to the inner membrane, whereas overproduced RnpB RNA is localized to the cytoplasm [Miczak91].

Structural properties of RnpA have been investigated [Gopalan97a]. The N terminus plays a role in the catalytic activity of RNAse P [Kim00a]. The RNR motif, centrally located in the RnpA protein, and the C-terminal region, are both required for interaction with the RnpB RNA [Kim00a]. RnpA is very basic [Hansen85]. The RnpA protein has been crystallized for structural studies [Choe03]. RnpA may interact with additional, non-RnpB RNAs [Lee02d].

An rnpA49 mutation results in a recessive heat sensitivity phenotype [Apirion80] and reduced RNAse P Kcat [Kirsebom89]. The rnp-241 also causes heat sensitivity [Hansen85]. An rnpA49 mutant harboring a ColE1-type episome exhibits buildup of RNA I-derived RNAs [Jung92]. R46H (C5A49) mutant protein shows defects in interaction with RnpB RNA [Baer89].

Mutations in RnpA affect RNAse P substrate specificity [Gopalan97]. The large-scale gene expression profile of an rnpA mutant has been evaluated [Li03]. Mutations in RnbP RNA and in the RnpA protein subunit of RNAse P exhibit different effects on tRNA precursor processing [Kirsebom88].

The gene encoding the RNAse P protein subunit has been isolated from Bacillus subtilis [Ogasawara85], Borrelia burgdorferi [Old93a], Synechocystis sp. PCC 6803 [Pascual96], Thermotoga maritima [Paul01a], Mycoplasma capricolum [Miyata93a], Proteus mirabilis [Skovgaard90], Micrococcus luteus [Fujita90], Buchnera aphidicola [Lai92, Hassan96, Clark98], Streptomyces coelicolor [Calcutt92], Streptomyces bikiniensis var. zorbonensis [Morse92], Mycobacterium leprae [Fsihi96], Xanthomonas campestris pv. campestris 17 [Yen02], Thermus thermophilus [Feltens03], and Thermus filiformis [Feltens03]. The RnpA protein exhibits antigenic similarity to proteins from human cells and from Bacillus subtilis [Mamula89]. E. coli rnpA mutant phenotypes are functionally complemented by RnpA from Synechocystis sp. PCC 6803 [Pascual96] or Streptomyces bikiniensis var. zorbonensis [Morse92], or by Brevibacterium albidum arginine tRNA [Kim97a].

Regulation has been described [Hansen85, Panagiotidis92].

Citations: [Ohnishi90, RiveraLeon95, Park98, Baer90]

Gene Citations: [Hansen82]

Locations: cytosol

Map Position: [3,882,516 -> 3,882,875] (83.68 centisomes, 301°)
Length: 360 bp / 119 aa

Molecular Weight of Polypeptide: 13.789 kD (from nucleotide sequence), 14 kD (experimental)

Unification Links: ASAP:ABE-0012117 , CGSC:267 , DIP:DIP-36039N , EchoBASE:EB0855 , EcoGene:EG10862 , EcoliWiki:b3704 , Mint:MINT-1218365 , ModBase:P0A7Y8 , OU-Microarray:b3704 , PortEco:rnpA , PR:PRO_000023798 , Protein Model Portal:P0A7Y8 , RefSeq:NP_418159 , RegulonDB:EG10862 , SMR:P0A7Y8 , String:511145.b3704 , UniProt:P0A7Y8

Relationship Links: InterPro:IN-FAMILY:IPR000100 , InterPro:IN-FAMILY:IPR014721 , InterPro:IN-FAMILY:IPR020539 , InterPro:IN-FAMILY:IPR020568 , PDB:Structure:2LJP , Pfam:IN-FAMILY:PF00825 , ProDom:IN-FAMILY:PD003629 , Prosite:IN-FAMILY:PS00648

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Biological Process: GO:0001682 - tRNA 5'-leader removal Inferred from experiment Inferred by computational analysis [GOA06, Chang75]
GO:0006396 - RNA processing Inferred from experiment [Bothwell76]
GO:0008033 - tRNA processing Inferred from experiment Inferred by computational analysis [UniProtGOA11a, GOA01a, Hansen85]
GO:0090501 - RNA phosphodiester bond hydrolysis Inferred from experiment [Li94]
GO:0090502 - RNA phosphodiester bond hydrolysis, endonucleolytic Inferred from experiment Inferred by computational analysis [GOA06, GOA01, GOA01a, Li94]
GO:0090305 - nucleic acid phosphodiester bond hydrolysis Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0004526 - ribonuclease P activity Inferred from experiment Inferred by computational analysis [GOA06, GOA01, GOA01a, Li94]
GO:0000049 - tRNA binding Inferred by computational analysis [GOA06, GOA01a]
GO:0003723 - RNA binding Inferred by computational analysis [UniProtGOA11a]
GO:0004518 - nuclease activity Inferred by computational analysis [UniProtGOA11a]
GO:0004519 - endonuclease activity Inferred by computational analysis [UniProtGOA11a]
GO:0016787 - hydrolase activity Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: information transfer RNA related RNA modification
metabolism degradation of macromolecules RNA

Essentiality data for rnpA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox No 37 Aerobic 7   No [Baba06, Comment 1]

Revised 25-May-2011 by Brito D

Subunit of: RNase P

Subunit composition of RNase P = [RnpB][RnpA]
         RnpB RNA; catalytic subunit of RNAse P = RnpB (extended summary available)
         RNase P protein component; processes tRNA, 4.5S RNA = RnpA (extended summary available)

Enzymatic reaction of: ribonuclease (RNase P)

EC Number:

a tRNA precursor with a 5' extension + H2O <=> an uncharged tRNA + a single-stranded RNA

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is irreversible in the direction shown.

In Pathways: tRNA processing

Enzymatic reaction of: ribonuclease (RNase P)

Synonyms: ribonuclease P, RNase P

EC Number:

a tRNA precursor with a 5' extension and a short 3' extension + H2O <=> a tRNA precursor with a short 3' extension + a single-stranded RNA

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

The reaction is irreversible in the direction shown.

In Pathways: tRNA processing

Cofactors or Prosthetic Groups: Mg2+ [Kazakov91]

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Units:

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram


10/20/97 Gene b3704 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10862; confirmed by SwissProt match.


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Ando03a: Ando T, Tanaka T, Kikuchi Y (2003). "Comparative analyses of hairpin substrate recognition by Escherichia coli and Bacillus subtilis ribonuclease P ribozymes." Biosci Biotechnol Biochem 67(8);1825-7. PMID: 12951523

Ando03b: Ando T, Tanaka T, Kikuchi Y (2003). "Bacterial ribonuclease P reaction is affected by substrate shape and magnesium ion concentration." Nucleic Acids Res Suppl (3);293-4. PMID: 14510496

Apirion80: Apirion D (1980). "Genetic mapping and some characterization of the rnpA49 mutation of Escherichia coli that affects the RNA-processing enzyme ribonuclease P." Genetics 94(2);291-9. PMID: 6156104

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Baer88: Baer MF, Reilly RM, McCorkle GM, Hai TY, Altman S, RajBhandary UL (1988). "The recognition by RNase P of precursor tRNAs." J Biol Chem 263(5);2344-51. PMID: 3123492

Baer89: Baer MF, Wesolowski D, Altman S (1989). "Characterization in vitro of the defect in a temperature-sensitive mutant of the protein subunit of RNase P from Escherichia coli." J Bacteriol 171(12);6862-6. PMID: 2480345

Baer90: Baer MF, Arnez JG, Guerrier-Takada C, Vioque A, Altman S (1990). "Preparation and characterization of RNase P from Escherichia coli." Methods Enzymol 181;569-82. PMID: 2199767

Bikoff75: Bikoff EK, Gefter ML (1975). "In vitro synthesis of transfer RNA. I. Purification of required components." J Biol Chem 250(16);6240-7. PMID: 1099089

Bikoff75a: Bikoff EK, LaRue BF, Gefter ML (1975). "In vitro synthesis of transfer RNA. II. Identification of required enzymatic activities." J Biol Chem 250(16);6248-55. PMID: 1099090

Biswas00: Biswas R, Ledman DW, Fox RO, Altman S, Gopalan V (2000). "Mapping RNA-protein interactions in ribonuclease P from Escherichia coli using disulfide-linked EDTA-Fe." J Mol Biol 296(1);19-31. PMID: 10656815

Bothwell76: Bothwell AL, Garber RL, Altman S (1976). "Nucleotide sequence and in vitro processing of a precursor molecule to Escherichia coli 4.5 S RNA." J Biol Chem 251(23);7709-16. PMID: 794064

Bothwell76a: Bothwell AL, Stark BC, Altman S (1976). "Ribonuclease P substrate specificity: cleavage of a bacteriophage phi80-induced RNA." Proc Natl Acad Sci U S A 73(6);1912-6. PMID: 778851

Brannvall02: Brannvall M, Fredrik Pettersson BM, Kirsebom LA (2002). "The residue immediately upstream of the RNase P cleavage site is a positive determinant." Biochimie 84(8);693-703. PMID: 12457557

Brannvall03: Brannvall M, Pettersson BM, Kirsebom LA (2003). "Importance of the +73/294 interaction in Escherichia coli RNase P RNA substrate complexes for cleavage and metal ion coordination." J Mol Biol 325(4);697-709. PMID: 12507473

Brannvall98: Brannvall M, Mattsson JG, Svard SG, Kirsebom LA (1998). "RNase P RNA structure and cleavage reflect the primary structure of tRNA genes." J Mol Biol 283(4);771-83. PMID: 9790839

Briani02: Briani F, Del Vecchio E, Migliorini D, Hajnsdorf E, Regnier P, Ghisotti D, Deho G (2002). "RNase E and polyadenyl polymerase I are involved in maturation of CI RNA, the P4 phage immunity factor." J Mol Biol 318(2);321-31. PMID: 12051840

Burgin90: Burgin AB, Pace NR (1990). "Mapping the active site of ribonuclease P RNA using a substrate containing a photoaffinity agent." EMBO J 9(12);4111-8. PMID: 1701142

Burkard88: Burkard U, Soll D (1988). "The unusually long amino acid acceptor stem of Escherichia coli selenocysteine tRNA results from abnormal cleavage by RNase P." Nucleic Acids Res 16(24);11617-24. PMID: 3062578

Burkard88a: Burkard U, Willis I, Soll D (1988). "Processing of histidine transfer RNA precursors. Abnormal cleavage site for RNase P." J Biol Chem 263(5);2447-51. PMID: 3276688

Busch00: Busch S, Kirsebom LA, Notbohm H, Hartmann RK (2000). "Differential role of the intermolecular base-pairs G292-C(75) and G293-C(74) in the reaction catalyzed by Escherichia coli RNase P RNA." J Mol Biol 299(4);941-51. PMID: 10843849

Calcutt92: Calcutt MJ, Schmidt FJ (1992). "Conserved gene arrangement in the origin region of the Streptomyces coelicolor chromosome." J Bacteriol 174(10);3220-6. PMID: 1577691

Chang75: Chang S, Carbon J (1975). "The nucleotide sequence of a precursor to the glycine- and threonine-specific transfer ribonucleic acids of Escherichia coli." J Biol Chem 250(14);5542-55. PMID: 167017

Choe03: Choe HW, Jeong DG, Park JH, Schlesinger R, Labahn J, Hofmann KP, Buldt G (2003). "Preliminary X-ray characterization of the ribonuclease P (C5 protein) from Escherichia coli: expression, crystallization and cryoconditions." Acta Crystallogr D Biol Crystallogr 59(Pt 2);350-2. PMID: 12554950

Christian98: Christian EL, McPheeters DS, Harris ME (1998). "Identification of individual nucleotides in the bacterial ribonuclease P ribozyme adjacent to the pre-tRNA cleavage site by short-range photo-cross-linking." Biochemistry 37(50);17618-28. PMID: 9860878

Clark98: Clark MA, Baumann L, Baumann P (1998). "Sequence analysis of a 34.7-kb DNA segment from the genome of Buchnera aphidicola (endosymbiont of aphids) containing groEL, dnaA, the atp operon, gidA, and rho." Curr Microbiol 36(3);158-63. PMID: 9516544

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Eubank02: Eubank TD, Biswas R, Jovanovic M, Litovchick A, Lapidot A, Gopalan V (2002). "Inhibition of bacterial RNase P by aminoglycoside-arginine conjugates." FEBS Lett 511(1-3);107-12. PMID: 11821058

Feltens03: Feltens R, Gossringer M, Willkomm DK, Urlaub H, Hartmann RK (2003). "An unusual mechanism of bacterial gene expression revealed for the RNase P protein of Thermus strains." Proc Natl Acad Sci U S A 100(10);5724-9. PMID: 12719542

Fsihi96: Fsihi H, De Rossi E, Salazar L, Cantoni R, Labo M, Riccardi G, Takiff HE, Eiglmeier K, Bergh S, Cole ST (1996). "Gene arrangement and organization in a approximately 76 kb fragment encompassing the oriC region of the chromosome of Mycobacterium leprae." Microbiology 142 ( Pt 11);3147-61. PMID: 8969512

Fujita90: Fujita MQ, Yoshikawa H, Ogasawara N (1990). "Structure of the dnaA region of Micrococcus luteus: conservation and variations among eubacteria." Gene 93(1);73-8. PMID: 2172090

Furdon83: Furdon PJ, Guerrier-Takada C, Altman S (1983). "A G43 to U43 mutation in E. coli tRNAtyrsu3+ which affects processing by RNase P." Nucleic Acids Res 11(5);1491-505. PMID: 6298744

Gaur93: Gaur RK, Krupp G (1993). "Modification interference approach to detect ribose moieties important for the optimal activity of a ribozyme." Nucleic Acids Res 21(1);21-6. PMID: 8441616

Gaur96: Gaur RK, Hanne A, Conrad F, Kahle D, Krupp G (1996). "Differences in the interaction of Escherichia coli RNase P RNA with tRNAs containing a short or a long extra arm." RNA 2(7);674-81. PMID: 8756410

GOA01: GOA, MGI (2001). "Gene Ontology annotation based on Enzyme Commission mapping." Genomics 74;121-128.

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Gopalan97: Gopalan V, Baxevanis AD, Landsman D, Altman S (1997). "Analysis of the functional role of conserved residues in the protein subunit of ribonuclease P from Escherichia coli." J Mol Biol 267(4);818-29. PMID: 9135114

Gopalan97a: Gopalan V, Golbik R, Schreiber G, Fersht AR, Altman S (1997). "Fluorescence properties of a tryptophan residue in an aromatic core of the protein subunit of ribonuclease P from Escherichia coli." J Mol Biol 267(4);765-9. PMID: 9135109

Gopalan99: Gopalan V, Kuhne H, Biswas R, Li H, Brudvig GW, Altman S (1999). "Mapping RNA-protein interactions in ribonuclease P from Escherichia coli using electron paramagnetic resonance spectroscopy." Biochemistry 38(6);1705-14. PMID: 10026248

Green88a: Green CJ, Vold BS (1988). "Structural requirements for processing of synthetic tRNAHis precursors by the catalytic RNA component of RNase P." J Biol Chem 263(2);652-7. PMID: 2447080

GuerrierTakada83: Guerrier-Takada C, Gardiner K, Marsh T, Pace N, Altman S (1983). "The RNA moiety of ribonuclease P is the catalytic subunit of the enzyme." Cell 35(3 Pt 2);849-57. PMID: 6197186

GuerrierTakada84: Guerrier-Takada C, Altman S (1984). "Catalytic activity of an RNA molecule prepared by transcription in vitro." Science 223(4633);285-6. PMID: 6199841

GuerrierTakada84a: Guerrier-Takada C, McClain WH, Altman S (1984). "Cleavage of tRNA precursors by the RNA subunit of E. coli ribonuclease P (M1 RNA) is influenced by 3'-proximal CCA in the substrates." Cell 38(1);219-24. PMID: 6380759

GuerrierTakada86: Guerrier-Takada C, Haydock K, Allen L, Altman S (1986). "Metal ion requirements and other aspects of the reaction catalyzed by M1 RNA, the RNA subunit of ribonuclease P from Escherichia coli." Biochemistry 25(7);1509-15. PMID: 2423112

GuerrierTakada88: Guerrier-Takada C, van Belkum A, Pleij CW, Altman S (1988). "Novel reactions of RNAase P with a tRNA-like structure in turnip yellow mosaic virus RNA." Cell 53(2);267-72. PMID: 3359488

GuerrierTakada89: Guerrier-Takada C, Lumelsky N, Altman S (1989). "Specific interactions in RNA enzyme-substrate complexes." Science 246(4937);1578-84. PMID: 2480641

GuerrierTakada93: Guerrier-Takada C, Altman S (1993). "A physical assay for and kinetic analysis of the interactions between M1 RNA and tRNA precursor substrates." Biochemistry 32(28);7152-61. PMID: 7688247

Gurevitz83: Gurevitz M, Apirion D (1983). "Interplay among processing and degradative enzymes and a precursor ribonucleic acid in the selective maturation and maintenance of ribonucleic acid molecules." Biochemistry 22(17);4000-5. PMID: 6351914

Hansen01b: Hansen A, Pfeiffer T, Zuleeg T, Limmer S, Ciesiolka J, Feltens R, Hartmann RK (2001). "Exploring the minimal substrate requirements for trans-cleavage by RNase P holoenzymes from Escherichia coli and Bacillus subtilis." Mol Microbiol 41(1);131-43. PMID: 11454206

Hansen82: Hansen FG, Hansen EB, Atlung T (1982). "The nucleotide sequence of the dnaA gene promoter and of the adjacent rpmH gene, coding for the ribosomal protein L34, of Escherichia coli." EMBO J 1982;1(9);1043-8. PMID: 6329723

Hansen85: Hansen FG, Hansen EB, Atlung T (1985). "Physical mapping and nucleotide sequence of the rnpA gene that encodes the protein component of ribonuclease P in Escherichia coli." Gene 38(1-3);85-93. PMID: 2415431

Hardt93: Hardt WD, Schlegl J, Erdmann VA, Hartmann RK (1993). "Gel retardation analysis of E. coli M1 RNA-tRNA complexes." Nucleic Acids Res 21(15);3521-7. PMID: 7688454

Hardt93a: Hardt WD, Schlegl J, Erdmann VA, Hartmann RK (1993). "Role of the D arm and the anticodon arm in tRNA recognition by eubacterial and eukaryotic RNase P enzymes." Biochemistry 32(48);13046-53. PMID: 7694652

Hardt95: Hardt WD, Schlegl J, Erdmann VA, Hartmann RK (1995). "Kinetics and thermodynamics of the RNase P RNA cleavage reaction: analysis of tRNA 3'-end variants." J Mol Biol 247(2);161-72. PMID: 7535857

Hardt95a: Hardt WD, Warnecke JM, Erdmann VA, Hartmann RK (1995). "Rp-phosphorothioate modifications in RNase P RNA that interfere with tRNA binding." EMBO J 14(12);2935-44. PMID: 7540978

Hardt96: Hardt WD, Erdmann VA, Hartmann RK (1996). "Rp-deoxy-phosphorothioate modification interference experiments identify 2'-OH groups in RNase P RNA that are crucial to tRNA binding." RNA 2(12);1189-98. PMID: 8972769

Harris97: Harris ME, Kazantsev AV, Chen JL, Pace NR (1997). "Analysis of the tertiary structure of the ribonuclease P ribozyme-substrate complex by site-specific photoaffinity crosslinking." RNA 3(6);561-76. PMID: 9174092

Hartmann95: Hartmann RK, Heinrich J, Schlegl J, Schuster H (1995). "Precursor of C4 antisense RNA of bacteriophages P1 and P7 is a substrate for RNase P of Escherichia coli." Proc Natl Acad Sci U S A 92(13);5822-6. PMID: 7597035

Hassan96: Hassan AK, Moriya S, Baumann P, Yoshikawa H, Ogasawara N (1996). "Structure of the dnaA region of the endosymbiont, Buchnera aphidicola, of aphid Schizaphis graminum." DNA Res 3(6);415-9. PMID: 9097044

Heide01: Heide C, Busch S, Feltens R, Hartmann RK (2001). "Distinct modes of mature and precursor tRNA binding to Escherichia coli RNase P RNA revealed by NAIM analyses." RNA 7(4);553-64. PMID: 11345434

Heide01a: Heide C, Feltens R, Hartmann RK (2001). "Purine N7 groups that are crucial to the interaction of Escherichia coli rnase P RNA with tRNA." RNA 7(7);958-68. PMID: 11453068

Heide99: Heide C, Pfeiffer T, Nolan JM, Hartmann RK (1999). "Guanosine 2-NH2 groups of Escherichia coli RNase P RNA involved in intramolecular tertiary contacts and direct interactions with tRNA." RNA 5(1);102-16. PMID: 9917070

Holm92: Holm PS, Krupp G (1992). "The acceptor stem in pre-tRNAs determines the cleavage specificity of RNase P." Nucleic Acids Res 20(3);421-3. PMID: 1371348

Hori01: Hori Y, Bichenkova EV, Wilton AN, El-Attug MN, Sadat-Ebrahimi S, Tanaka T, Kikuchi Y, Araki M, Sugiura Y, Douglas KT (2001). "Synthetic inhibitors of the processing of pretransfer RNA by the ribonuclease P ribozyme: enzyme inhibitors which act by binding to substrate." Biochemistry 40(3);603-8. PMID: 11170376

Ilgen76: Ilgen C, Kirk LL, Carbon J (1976). "Isolation and characterization of large transfer ribonucleic acid precursors from Escherichia coli." J Biol Chem 251(4);922-9. PMID: 765341

Jung92: Jung YH, Park I, Lee Y (1992). "Alteration of RNA I metabolism in a temperature-sensitive Escherichia coli rnpA mutant strain." Biochem Biophys Res Commun 186(3);1463-70. PMID: 1380803

Kaye02: Kaye NM, Christian EL, Harris ME (2002). "NAIM and site-specific functional group modification analysis of RNase P RNA: magnesium dependent structure within the conserved P1-P4 multihelix junction contributes to catalysis." Biochemistry 41(14);4533-45. PMID: 11926814

Kazakov91: Kazakov S, Altman S (1991). "Site-specific cleavage by metal ion cofactors and inhibitors of M1 RNA, the catalytic subunit of RNase P from Escherichia coli." Proc Natl Acad Sci U S A 88(20);9193-7. PMID: 1718000

Kim00a: Kim M, Hyun Park B, Lee Y (2000). "Effects of terminal deletions in C5 protein on promoting RNase P catalysis." Biochem Biophys Res Commun 268(1);118-23. PMID: 10652223

Kim97a: Kim MS, Kim S, Kim SC, Lee YM, Jeon ES, Park CU, Lee Y (1997). "The Brevibacterium albidum gene encoding the arginine tRNACCG complements the growth defect of an Escherichia coli strain carrying a thermosensitive mutation in the rnpA gene at the nonpermissive temperature." Mol Gen Genet 254(4);464-8. PMID: 9180701

Kirsebom88: Kirsebom LA, Baer MF, Altman S (1988). "Differential effects of mutations in the protein and RNA moieties of RNase P on the efficiency of suppression by various tRNA suppressors." J Mol Biol 204(4);879-88. PMID: 2464697

Kirsebom89: Kirsebom LA, Altman S (1989). "Reaction in vitro of some mutants of RNase P with wild-type and temperature-sensitive substrates." J Mol Biol 207(4);837-40. PMID: 2474662

Kirsebom92: Kirsebom LA, Svard SG (1992). "The kinetics and specificity of cleavage by RNase P is mainly dependent on the structure of the amino acid acceptor stem." Nucleic Acids Res 20(3);425-32. PMID: 1371349

Kirsebom93: Kirsebom LA, Svard SG (1993). "Identification of a region within M1 RNA of Escherichia coli RNase P important for the location of the cleavage site on a wild-type tRNA precursor." J Mol Biol 231(3);594-604. PMID: 7685824

Kirsebom94: Kirsebom LA, Svard SG (1994). "Base pairing between Escherichia coli RNase P RNA and its substrate." EMBO J 13(20);4870-6. PMID: 7525271

Kleineidam93: Kleineidam RG, Pitulle C, Sproat B, Krupp G (1993). "Efficient cleavage of pre-tRNAs by E. coli RNAse P RNA requires the 2'-hydroxyl of the ribose at the cleavage site." Nucleic Acids Res 21(5);1097-101. PMID: 7681942

Knap90: Knap AK, Wesolowski D, Altman S (1990). "Protection from chemical modification of nucleotides in complexes of M1 RNA, the catalytic subunit of RNase P from E coli, and tRNA precursors." Biochimie 72(11);779-90. PMID: 1707681

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