Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
Metabolic Modeling Tutorial
discounted EARLY registration ends Dec 31, 2014
BioCyc websites down
12/28 - 12/31
for maintenance.
twitter

Escherichia coli K-12 substr. MG1655 Polypeptide: 50S ribosomal subunit protein L1



Gene: rplA Accession Numbers: EG10864 (EcoCyc), b3984, ECK3975

Synonyms: rpy

Regulation Summary Diagram: ?

Component of:
50S ribosomal subunit
ribosome (summary available)

Summary:
The L1 protein is a component of the 50S subunit of the ribosome and also functions in the post-transcriptional regulation of the ribosomal protein genes encoded in the L11 operon. Ribosomes lacking L1 show a lower translation activity than wild type [Subramanian80] and are defective in binding of aminoacylated tRNA [Sander83]. L1 has been identified within a 3-D map of the 70S ribosome constructed by cryo-electron microscopy [Malhotra98].

L1 interacts with a region in the 5' end of 23S rRNA [Branlant76, Branlant80, Egebjerg91]. It also can be crosslinked to a region near the central fold of aminoacylated tRNA in the P and E site [Podkowinski89, Rosen93, Osswald95]. L1 is located within 21 Å of nucleotide C2475 of 23S rRNA, near the peptidyltransferase center [Muralikrishna95].

L1 is a translational repressor of the synthesis of L11 and L1, the proteins encoded by the L11 operon [Brot81, Thomas87, Stoffler81, Yates80, Dabbs81]. Synthesis of L1 is regulated by translational coupling to the synthesis of L11 [Yates81, Baughman83]. The target site for L1 binding to the mRNA is near the translation initiation site of L11 [Yates81, Baughman83, Thomas87b], and the presence of 23S rRNA relieves translational inhibition by L1 [Yates81]. The predicted secondary structure of the L1 binding region within 23S rRNA and rplKA mRNA is similar [Branlant80, Gourse81] and has been studied experimentally [Baughman84, Kearney87, Said88, Drygin00].

Both the growth rate control and stringent control of the synthesis of ribosomal proteins L11 and L1 are resulting from translational regulation by L1 [Cole86].

Gene Citations: [Steward91]

Locations: cytosol, ribosome

Map Position: [4,176,902 -> 4,177,606] (90.03 centisomes)
Length: 705 bp / 234 aa

Molecular Weight of Polypeptide: 24.73 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0013031 , CGSC:263 , DIP:DIP-35746N , EchoBASE:EB0857 , EcoGene:EG10864 , EcoliWiki:b3984 , Mint:MINT-1268287 , ModBase:P0A7L0 , OU-Microarray:b3984 , PortEco:rplA , PR:PRO_000023809 , Pride:P0A7L0 , Protein Model Portal:P0A7L0 , RefSeq:NP_418411 , RegulonDB:EG10864 , SMR:P0A7L0 , String:511145.b3984 , Swiss-Model:P0A7L0 , UniProt:P0A7L0

Relationship Links: InterPro:IN-FAMILY:IPR002143 , InterPro:IN-FAMILY:IPR005878 , InterPro:IN-FAMILY:IPR016094 , InterPro:IN-FAMILY:IPR016095 , InterPro:IN-FAMILY:IPR023673 , InterPro:IN-FAMILY:IPR023674 , InterPro:IN-FAMILY:IPR028364 , PDB:Structure:1EG0 , PDB:Structure:1ML5 , PDB:Structure:2GYA , PDB:Structure:2GYC , PDB:Structure:2RDO , PDB:Structure:2WWQ , PDB:Structure:3E1B , PDB:Structure:3E1D , PDB:Structure:3FIK , PDB:Structure:3IZT , PDB:Structure:3IZU , PDB:Structure:3J01 , PDB:Structure:3J0T , PDB:Structure:3J0W , PDB:Structure:3J0Y , PDB:Structure:3J11 , PDB:Structure:3J12 , PDB:Structure:3J14 , PDB:Structure:3J37 , PDB:Structure:3KCR , PDB:Structure:487D , Pfam:IN-FAMILY:PF00687 , Prosite:IN-FAMILY:PS01199

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0045947 - negative regulation of translational initiation Inferred from experiment [Baughman83]
GO:0006412 - translation Inferred by computational analysis [GOA06, GOA01]
GO:0006417 - regulation of translation Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0005515 - protein binding Inferred from experiment [Arifuzzaman06, Butland05]
GO:0000049 - tRNA binding Inferred by computational analysis [UniProtGOA11a]
GO:0003723 - RNA binding Inferred by computational analysis [UniProtGOA11a, GOA01]
GO:0003735 - structural constituent of ribosome Inferred by computational analysis [GOA01]
GO:0019843 - rRNA binding Inferred by computational analysis [UniProtGOA11a, GOA06]
Cellular Component: GO:0005829 - cytosol Inferred from experiment Inferred by computational analysis [DiazMejia09, Ishihama08, LopezCampistrou05]
GO:0022625 - cytosolic large ribosomal subunit Inferred from experiment [Hindennach71]
GO:0005840 - ribosome Inferred by computational analysis [UniProtGOA11a]
GO:0015934 - large ribosomal subunit Inferred by computational analysis [GOA01]
GO:0030529 - ribonucleoprotein complex Inferred by computational analysis [UniProtGOA11a]

MultiFun Terms: cell structure ribosomes
information transfer protein related ribosomal proteins
information transfer protein related translation
regulation type of regulation posttranscriptional

Regulated Transcription Units (1 total): ?

Notes:

Essentiality data for rplA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Credits:
Last-Curated ? 30-Mar-2006 by Keseler I , SRI International


Subunit of: 50S ribosomal subunit

Synonyms: ribosome, large subunit

Subunit composition of 50S ribosomal subunit = [RrlA][RrfA][RplA][RplB][RplC][RplD][RplE][RplF][(RplJ)([RplL]2)2][RplI][RplK][RplM][RplN][RplO][RplP][RplQ][RplR][RplS][RplT][RplU][RplV][RplW][RplX][RplY][RpmA][RpmB][RpmC][RpmD][RpmE][RpmF][RpmG][RpmH][RpmI][RpmJ]
         23S ribosomal RNA (rrlA) = RrlA (extended summary available)
         5S ribosomal RNA (rrfA) = RrfA (extended summary available)
         50S ribosomal subunit protein L1 = RplA (extended summary available)
         50S ribosomal subunit protein L2 = RplB (summary available)
         50S ribosomal subunit protein L3 = RplC (summary available)
         50S ribosomal subunit protein L4 = RplD (extended summary available)
         50S ribosomal subunit protein L5 = RplE (summary available)
         50S ribosomal subunit protein L6 = RplF (summary available)
         50S ribosomal protein complex L8 = (RplJ)([RplL]2)2 (summary available)
                 50S ribosomal subunit protein L10 = RplJ (extended summary available)
                 50S ribosomal subunit protein L7/L12 dimer = (RplL)2
                         50S ribosomal subunit protein L12 = RplL
         50S ribosomal subunit protein L9 = RplI (summary available)
         50S ribosomal subunit protein L11 = RplK (extended summary available)
         50S ribosomal subunit protein L13 = RplM (extended summary available)
         50S ribosomal subunit protein L14 = RplN (extended summary available)
         50S ribosomal subunit protein L15 = RplO (summary available)
         50S ribosomal subunit protein L16 = RplP (extended summary available)
         50S ribosomal subunit protein L17 = RplQ (summary available)
         50S ribosomal subunit protein L18 = RplR (extended summary available)
         50S ribosomal subunit protein L19 = RplS (extended summary available)
         50S ribosomal subunit protein L20 = RplT (extended summary available)
         50S ribosomal subunit protein L21 = RplU (summary available)
         50S ribosomal subunit protein L22 = RplV (extended summary available)
         50S ribosomal subunit protein L23 = RplW (extended summary available)
         50S ribosomal subunit protein L24 = RplX (summary available)
         50S ribosomal subunit protein L25 = RplY (summary available)
         50S ribosomal subunit protein L27 = RpmA (extended summary available)
         50S ribosomal subunit protein L28 = RpmB (summary available)
         50S ribosomal subunit protein L29 = RpmC (summary available)
         50S ribosomal subunit protein L30 = RpmD (summary available)
         50S ribosomal subunit protein L31 = RpmE (summary available)
         50S ribosomal subunit protein L32 = RpmF (summary available)
         50S ribosomal subunit protein L33 = RpmG (summary available)
         50S ribosomal subunit protein L34 = RpmH (summary available)
         50S ribosomal subunit protein L35 = RpmI (summary available)
         50S ribosomal subunit protein L36 = RpmJ (summary available)

Component of: ribosome (summary available)

Relationship Links: PDB:Structure:1P85 , PDB:Structure:1P86 , PDB:Structure:2AW4 , PDB:Structure:2AWB

Credits:
Created 02-Jun-2006 by Keseler I , SRI International


Subunit of: ribosome

Subunit composition of ribosome = [(RrsA)(RpsA)(RpsB)(RpsC)(RpsD)(RpsE)(RpsF)(RpsG)(RpsH)(RpsI)(RpsJ)(RpsK)(RpsL)(RpsM)(RpsN)(RpsO)(RpsP)(RpsQ)(RpsR)(RpsS)(RpsT)(RpsU)(Sra)][(RrlA)(RrfA)(RplA)(RplB)(RplC)(RplD)(RplE)(RplF)([RplJ][(RplL)2]2)(RplI)(RplK)(RplM)(RplN)(RplO)(RplP)(RplQ)(RplR)(RplS)(RplT)(RplU)(RplV)(RplW)(RplX)(RplY)(RpmA)(RpmB)(RpmC)(RpmD)(RpmE)(RpmF)(RpmG)(RpmH)(RpmI)(RpmJ)]
         30S ribosomal subunit = (RrsA)(RpsA)(RpsB)(RpsC)(RpsD)(RpsE)(RpsF)(RpsG)(RpsH)(RpsI)(RpsJ)(RpsK)(RpsL)(RpsM)(RpsN)(RpsO)(RpsP)(RpsQ)(RpsR)(RpsS)(RpsT)(RpsU)(Sra) (summary available)
                 16S ribosomal RNA (rrsA) = RrsA (extended summary available)
                 30S ribosomal subunit protein S1 = RpsA (extended summary available)
                 30S ribosomal subunit protein S2 = RpsB (summary available)
                 30S ribosomal subunit protein S3 = RpsC (summary available)
                 30S ribosomal subunit protein S4 = RpsD (extended summary available)
                 30S ribosomal subunit protein S5 = RpsE (extended summary available)
                 30S ribosomal subunit protein S6 = RpsF (extended summary available)
                 30S ribosomal subunit protein S7 = RpsG (extended summary available)
                 30S ribosomal subunit protein S8 = RpsH (extended summary available)
                 30S ribosomal subunit protein S9 = RpsI (extended summary available)
                 30S ribosomal subunit protein S10 = RpsJ (extended summary available)
                 30S ribosomal subunit protein S11 = RpsK (summary available)
                 30S ribosomal subunit protein S12 = RpsL (extended summary available)
                 30S ribosomal subunit protein S13 = RpsM (extended summary available)
                 30S ribosomal subunit protein S14 = RpsN (summary available)
                 30S ribosomal subunit protein S15 = RpsO (extended summary available)
                 30S ribosomal subunit protein S16 = RpsP (summary available)
                 30S ribosomal subunit protein S17 = RpsQ (summary available)
                 30S ribosomal subunit protein S18 = RpsR (extended summary available)
                 30S ribosomal subunit protein S19 = RpsS (summary available)
                 30S ribosomal subunit protein S20 = RpsT (extended summary available)
                 30S ribosomal subunit protein S21 = RpsU (summary available)
                 30S ribosomal subunit protein S22 = Sra (summary available)
         50S ribosomal subunit = (RrlA)(RrfA)(RplA)(RplB)(RplC)(RplD)(RplE)(RplF)([RplJ][(RplL)2]2)(RplI)(RplK)(RplM)(RplN)(RplO)(RplP)(RplQ)(RplR)(RplS)(RplT)(RplU)(RplV)(RplW)(RplX)(RplY)(RpmA)(RpmB)(RpmC)(RpmD)(RpmE)(RpmF)(RpmG)(RpmH)(RpmI)(RpmJ)
                 23S ribosomal RNA (rrlA) = RrlA (extended summary available)
                 5S ribosomal RNA (rrfA) = RrfA (extended summary available)
                 50S ribosomal subunit protein L1 = RplA (extended summary available)
                 50S ribosomal subunit protein L2 = RplB (summary available)
                 50S ribosomal subunit protein L3 = RplC (summary available)
                 50S ribosomal subunit protein L4 = RplD (extended summary available)
                 50S ribosomal subunit protein L5 = RplE (summary available)
                 50S ribosomal subunit protein L6 = RplF (summary available)
                 50S ribosomal protein complex L8 = (RplJ)([RplL]2)2 (summary available)
                         50S ribosomal subunit protein L10 = RplJ (extended summary available)
                         50S ribosomal subunit protein L7/L12 dimer = (RplL)2
                                 50S ribosomal subunit protein L12 = RplL
                 50S ribosomal subunit protein L9 = RplI (summary available)
                 50S ribosomal subunit protein L11 = RplK (extended summary available)
                 50S ribosomal subunit protein L13 = RplM (extended summary available)
                 50S ribosomal subunit protein L14 = RplN (extended summary available)
                 50S ribosomal subunit protein L15 = RplO (summary available)
                 50S ribosomal subunit protein L16 = RplP (extended summary available)
                 50S ribosomal subunit protein L17 = RplQ (summary available)
                 50S ribosomal subunit protein L18 = RplR (extended summary available)
                 50S ribosomal subunit protein L19 = RplS (extended summary available)
                 50S ribosomal subunit protein L20 = RplT (extended summary available)
                 50S ribosomal subunit protein L21 = RplU (summary available)
                 50S ribosomal subunit protein L22 = RplV (extended summary available)
                 50S ribosomal subunit protein L23 = RplW (extended summary available)
                 50S ribosomal subunit protein L24 = RplX (summary available)
                 50S ribosomal subunit protein L25 = RplY (summary available)
                 50S ribosomal subunit protein L27 = RpmA (extended summary available)
                 50S ribosomal subunit protein L28 = RpmB (summary available)
                 50S ribosomal subunit protein L29 = RpmC (summary available)
                 50S ribosomal subunit protein L30 = RpmD (summary available)
                 50S ribosomal subunit protein L31 = RpmE (summary available)
                 50S ribosomal subunit protein L32 = RpmF (summary available)
                 50S ribosomal subunit protein L33 = RpmG (summary available)
                 50S ribosomal subunit protein L34 = RpmH (summary available)
                 50S ribosomal subunit protein L35 = RpmI (summary available)
                 50S ribosomal subunit protein L36 = RpmJ (summary available)

Summary:
The ribosome is a complex machinery that translates the genetic code.

A crystal structure of the E. coli ribosome has been determined at 3.5 Å resolution [Schuwirth05]. Additional crystal structures of the ribosome with tRNA bound in two functionally distinct states reveal how a ratchet-like motion of the small and large subunits contributes to translocation, termination of translation, and ribosome recycling [Zhang09b, Dunkle11].

Approximately eight molecules of Zn2+ are bound to the ribosome; therefore, it appears that a large fraction of intracellular Zn2+ is ribosome-associated [Hensley11].

Selected reviews: [Ramakrishnan02, Yonath05, Ogle05, Kaczanowska07]

Citations: [Kuhlenkoetter11]

Relationship Links: PDB:Structure:3R8N , PDB:Structure:3R8O , PDB:Structure:3R8S , PDB:Structure:3R8T

Credits:
Created 15-Jun-2006 by Keseler I , SRI International


Sequence Features

Feature Class Location Citations Comment
Cleavage-of-Initial-Methionine 1
[Link97, Brauer78]
 
Chain 2 -> 234
[UniProt09]
UniProt: 50S ribosomal protein L1;
Sequence-Conflict 12
[Link97, UniProt10a]
Alternate sequence: R → I; UniProt: (in Ref. 7; AA sequence);
N6-succinyllysine-Modification 105
[Zhang11, UniProt12]
UniProt: N6-succinyllysine.
N6-succinyllysine-Modification 154
[Zhang11, UniProt12]
UniProt: N6-succinyllysine.
N6-succinyllysine-Modification 186
[Zhang11, UniProt12]
UniProt: N6-succinyllysine.
N6-succinyllysine-Modification 197
[Zhang11, UniProt12]
UniProt: N6-succinyllysine.
Acetylation-Modification 197
[Yu08]
 


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
10/20/97 Gene b3984 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10864; confirmed by SwissProt match.


References

Arifuzzaman06: Arifuzzaman M, Maeda M, Itoh A, Nishikata K, Takita C, Saito R, Ara T, Nakahigashi K, Huang HC, Hirai A, Tsuzuki K, Nakamura S, Altaf-Ul-Amin M, Oshima T, Baba T, Yamamoto N, Kawamura T, Ioka-Nakamichi T, Kitagawa M, Tomita M, Kanaya S, Wada C, Mori H (2006). "Large-scale identification of protein-protein interaction of Escherichia coli K-12." Genome Res 16(5);686-91. PMID: 16606699

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Baughman83: Baughman G, Nomura M (1983). "Localization of the target site for translational regulation of the L11 operon and direct evidence for translational coupling in Escherichia coli." Cell 34(3);979-88. PMID: 6354472

Baughman84: Baughman G, Nomura M (1984). "Translational regulation of the L11 ribosomal protein operon of Escherichia coli: analysis of the mRNA target site using oligonucleotide-directed mutagenesis." Proc Natl Acad Sci U S A 81(17);5389-93. PMID: 6382263

Branlant76: Branlant C, Krol A, Sriwdada J, Ebel JP, Sloof P, Garrett RA (1976). "The binding site of protein L1 ON 23-S ribosomal RNA of Escherichia coli. 2. Identification of the rna region contained in the L1 ribonucleoproteins and determination of the order of the RNA subfragments within this region." Eur J Biochem 70(2);457-69. PMID: 827439

Branlant80: Branlant C, Krol A, Ebel JP (1980). "Characterization of the Escherichia coli 23S Ribosomal RNA region associated with ribosomal protein L1. Evidence for homologies with the 5'-end region of the L11 operon." Nucleic Acids Res 8(23);5567-77. PMID: 6162152

Brauer78: Brauer D, Ochsner I (1978). "The primary structure of protein L1 from the large ribosomal subunit of Escherichia coli." FEBS Lett 96(2);317-21. PMID: 365581

Brot81: Brot N, Caldwell P, Weissbach H (1981). "Regulation of synthesis of Escherichia coli ribosomal proteins L1 and L11." Arch Biochem Biophys 206(1);51-3. PMID: 7011209

Butland05: Butland G, Peregrin-Alvarez JM, Li J, Yang W, Yang X, Canadien V, Starostine A, Richards D, Beattie B, Krogan N, Davey M, Parkinson J, Greenblatt J, Emili A (2005). "Interaction network containing conserved and essential protein complexes in Escherichia coli." Nature 433(7025);531-7. PMID: 15690043

Cole86: Cole JR, Nomura M (1986). "Translational regulation is responsible for growth-rate-dependent and stringent control of the synthesis of ribosomal proteins L11 and L1 in Escherichia coli." Proc Natl Acad Sci U S A 83(12);4129-33. PMID: 3520566

Dabbs81: Dabbs ER, Ehrlich R, Hasenbank R, Schroeter BH, Stoffler-Meilicke M, Stoffler G (1981). "Mutants of Escherichia coli lacking ribosomal protein L1." J Mol Biol 149(4);553-78. PMID: 6171646

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Drygin00: Drygin D, Zimmermann RA (2000). "Magnesium ions mediate contacts between phosphoryl oxygens at positions 2122 and 2176 of the 23S rRNA and ribosomal protein L1." RNA 6(12);1714-26. PMID: 11142372

Dunkle11: Dunkle JA, Wang L, Feldman MB, Pulk A, Chen VB, Kapral GJ, Noeske J, Richardson JS, Blanchard SC, Cate JH (2011). "Structures of the bacterial ribosome in classical and hybrid states of tRNA binding." Science 332(6032);981-4. PMID: 21596992

Egebjerg91: Egebjerg J, Christiansen J, Garrett RA (1991). "Attachment sites of primary binding proteins L1, L2 and L23 on 23 S ribosomal RNA of Escherichia coli." J Mol Biol 222(2);251-64. PMID: 1960726

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Gourse81: Gourse RL, Thurlow DL, Gerbi SA, Zimmermann RA (1981). "Specific binding of a prokaryotic ribosomal protein to a eukaryotic ribosomal RNA: implications for evolution and autoregulation." Proc Natl Acad Sci U S A 78(5);2722-6. PMID: 6265904

Hensley11: Hensley MP, Tierney DL, Crowder MW (2011). "Zn(II) binding to Escherichia coli 70S ribosomes." Biochemistry 50(46);9937-9. PMID: 22026583

Hindennach71: Hindennach I, Kaltschmidt E, Wittmann HG (1971). "Ribosomal proteins. Isolation of proteins from 50S ribosomal subunits of Escherichia coli." Eur J Biochem 23(1);12-6. PMID: 4942547

Ishihama08: Ishihama Y, Schmidt T, Rappsilber J, Mann M, Hartl FU, Kerner MJ, Frishman D (2008). "Protein abundance profiling of the Escherichia coli cytosol." BMC Genomics 9;102. PMID: 18304323

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Kaczanowska07: Kaczanowska M, Ryden-Aulin M (2007). "Ribosome biogenesis and the translation process in Escherichia coli." Microbiol Mol Biol Rev 71(3);477-94. PMID: 17804668

Kearney87: Kearney KR, Nomura M (1987). "Secondary structure of the autoregulatory mRNA binding site of ribosomal protein L1." Mol Gen Genet 210(1);60-8. PMID: 2448590

Kuhlenkoetter11: Kuhlenkoetter S, Wintermeyer W, Rodnina MV (2011). "Different substrate-dependent transition states in the active site of the ribosome." Nature 476(7360);351-4. PMID: 21804565

Link97: Link AJ, Robison K, Church GM (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18(8);1259-313. PMID: 9298646

LopezCampistrou05: Lopez-Campistrous A, Semchuk P, Burke L, Palmer-Stone T, Brokx SJ, Broderick G, Bottorff D, Bolch S, Weiner JH, Ellison MJ (2005). "Localization, annotation, and comparison of the Escherichia coli K-12 proteome under two states of growth." Mol Cell Proteomics 4(8);1205-9. PMID: 15911532

Malhotra98: Malhotra A, Penczek P, Agrawal RK, Gabashvili IS, Grassucci RA, Junemann R, Burkhardt N, Nierhaus KH, Frank J (1998). "Escherichia coli 70 S ribosome at 15 A resolution by cryo-electron microscopy: localization of fMet-tRNAfMet and fitting of L1 protein." J Mol Biol 280(1);103-16. PMID: 9653034

Muralikrishna95: Muralikrishna P, Cooperman BS (1995). "Ribosomal components neighboring the 2475 loop in Escherichia coli 50S subunits." Biochemistry 34(1);115-21. PMID: 7529559

Ogle05: Ogle JM, Ramakrishnan V (2005). "Structural insights into translational fidelity." Annu Rev Biochem 74;129-77. PMID: 15952884

Osswald95: Osswald M, Doring T, Brimacombe R (1995). "The ribosomal neighbourhood of the central fold of tRNA: cross-links from position 47 of tRNA located at the A, P or E site." Nucleic Acids Res 23(22);4635-41. PMID: 8524654

Podkowinski89: Podkowinski J, Gornicki P (1989). "Ribosomal proteins S7 and L1 are located close to the decoding site of E. coli ribosome--affinity labeling studies with modified tRNAs carrying photoreactive probes attached adjacent to the 3'-end of the anticodon." Nucleic Acids Res 17(21);8767-82. PMID: 2685749

Ramakrishnan02: Ramakrishnan V (2002). "Ribosome structure and the mechanism of translation." Cell 108(4);557-72. PMID: 11909526

Rosen93: Rosen KV, Alexander RW, Wower J, Zimmermann RA (1993). "Mapping the central fold of tRNA2(fMet) in the P site of the Escherichia coli ribosome." Biochemistry 32(47);12802-11. PMID: 8251501

Said88: Said B, Cole JR, Nomura M (1988). "Mutational analysis of the L1 binding site of 23S rRNA in Escherichia coli." Nucleic Acids Res 16(22);10529-45. PMID: 3060846

Sander83: Sander G (1983). "Ribosomal protein L1 from Escherichia coli. Its role in the binding of tRNA to the ribosome and in elongation factor g-dependent gtp hydrolysis." J Biol Chem 258(16);10098-103. PMID: 6350280

Schuwirth05: Schuwirth BS, Borovinskaya MA, Hau CW, Zhang W, Vila-Sanjurjo A, Holton JM, Cate JH (2005). "Structures of the bacterial ribosome at 3.5 A resolution." Science 310(5749);827-34. PMID: 16272117

Steward91: Steward KL, Linn T (1991). "In vivo analysis of overlapping transcription units in the rplKAJLrpoBC ribosomal protein-RNA polymerase gene cluster of Escherichia coli." J Mol Biol 218(1);23-31. PMID: 1825852

Stoffler81: Stoffler G, Hasenbank R, Dabbs ER (1981). "Expression of the L11-L1 operon in mutants of Escherichia coli lacking the ribosomal proteins L1 or L11." Mol Gen Genet 181(2);164-8. PMID: 7024735

Subramanian80: Subramanian AR, Dabbs ER (1980). "Functional studies on ribosomes lacking protein L1 from mutant Escherichia coli." Eur J Biochem 112(2);425-30. PMID: 7007045

Thomas87: Thomas MS, Bedwell DM, Nomura M (1987). "Regulation of alpha operon gene expression in Escherichia coli. A novel form of translational coupling." J Mol Biol 196(2);333-45. PMID: 3309351

Thomas87b: Thomas MS, Nomura M (1987). "Translational regulation of the L11 ribosomal protein operon of Escherichia coli: mutations that define the target site for repression by L1." Nucleic Acids Res 15(7);3085-96. PMID: 3104883

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt12: UniProt Consortium (2012). "UniProt version 2012-09 released on 2012-09-12 00:00:00." Database.

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Yates80: Yates JL, Arfsten AE, Nomura M (1980). "In vitro expression of Escherichia coli ribosomal protein genes: autogenous inhibition of translation." Proc Natl Acad Sci U S A 77(4);1837-41. PMID: 6445562

Yates81: Yates JL, Nomura M (1981). "Feedback regulation of ribosomal protein synthesis in E. coli: localization of the mRNA target sites for repressor action of ribosomal protein L1." Cell 24(1);243-9. PMID: 7016337

Yonath05: Yonath A (2005). "Antibiotics targeting ribosomes: resistance, selectivity, synergism and cellular regulation." Annu Rev Biochem 74;649-79. PMID: 16180279

Yu08: Yu BJ, Kim JA, Moon JH, Ryu SE, Pan JG (2008). "The diversity of lysine-acetylated proteins in Escherichia coli." J Microbiol Biotechnol 18(9);1529-36. PMID: 18852508

Zhang09b: Zhang W, Dunkle JA, Cate JH (2009). "Structures of the ribosome in intermediate states of ratcheting." Science 325(5943);1014-7. PMID: 19696352

Zhang11: Zhang Z, Tan M, Xie Z, Dai L, Chen Y, Zhao Y (2011). "Identification of lysine succinylation as a new post-translational modification." Nat Chem Biol 7(1);58-63. PMID: 21151122

Other References Related to Gene Regulation

Lemke11: Lemke JJ, Sanchez-Vazquez P, Burgos HL, Hedberg G, Ross W, Gourse RL (2011). "Direct regulation of Escherichia coli ribosomal protein promoters by the transcription factors ppGpp and DksA." Proc Natl Acad Sci U S A 108(14);5712-7. PMID: 21402902

Post79: Post LE, Strycharz GD, Nomura M, Lewis H, Dennis PP (1979). "Nucleotide sequence of the ribosomal protein gene cluster adjacent to the gene for RNA polymerase subunit beta in Escherichia coli." Proc Natl Acad Sci U S A 1979;76(4);1697-701. PMID: 377281

Ralling84: Ralling G, Linn T (1984). "Relative activities of the transcriptional regulatory sites in the rplKAJLrpoBC gene cluster of Escherichia coli." J Bacteriol 158(1);279-85. PMID: 6325390

Squires81: Squires C, Krainer A, Barry G, Shen WF, Squires CL (1981). "Nucleotide sequence at the end of the gene for the RNA polymerase beta' subunit (rpoC)." Nucleic Acids Res 1981;9(24);6827-40. PMID: 6278450

Yamamoto79: Yamamoto M, Nomura M (1979). "Organization of genes for transcription and translation in the rif region of the Escherichia coli chromosome." J Bacteriol 137(1);584-94. PMID: 368032


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Fri Dec 19, 2014, biocyc12.