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Escherichia coli K-12 substr. MG1655 Polypeptide: 50S ribosomal subunit protein L5



Gene: rplE Accession Numbers: EG10868 (EcoCyc), b3308, ECK3295

Regulation Summary Diagram: ?

Regulation summary diagram for rplE

Component of:
50S ribosomal subunit
ribosome (summary available)

Summary:
The L5 protein is a component of the 50S subunit of the ribosome. The crystal structure of the ribosome [Schuwirth05], cryo-EM reconstructions [Gao03], and crosslinking experiments [Lambert81] show that the L5 protein contacts the S13 protein of the small subunit of the ribosome, forming the only protein-protein bridge between the subunits. During the ratchet-like motion of the ribosome, L5 and S13 reorganize in different constellations [Shasmal10]. L5, L15 and L18 are required for 5S RNA incorporation into the 50S subunit of the ribosome [Rohl82], and L5 was shown to be required for the assembly of the central protuberance of the 50S subunit [Korepanov12].

L5 binds to 5S rRNA [Branlant76, ChenSchmeisser77, Zimmermann78, Spierer78, Spierer78a, Pieler82, Huber84]. The association of L5 with 5S rRNA is cooperatively stimulated by L18 [Spierer78], and the binding regions of the 5S rRNA-L5-L18 complex were mapped [Ostergaard98]. Both L5 and L18 are essential for viability [Korepanov07]. A crystal structure of the Thermus thermophilus L5 in complex with a fragment of 5S RNA has been solved [Perederina02]. L5 also crosslinks to IF-3 [Chaires82] and uncharged tRNA in the P site of the ribosome [Abdurashidova90, Podkowinski91, Osswald95]. Molecular dynamics simulations suggest that L5 facilitates tRNA translocation [Bock13].

L5 is altered in a thiopeptin-resistant mutant [Liou75]. L4, L5, and L21 bind to erythromycin cooperatively [Pye90]. Inhibition of the EF-G-ribosome complex by thiostrepton involves destabilization of 5S RNA-protein (L5 and L18) interactions [Hanson03].

Expression of L5 is regulated at the translational level by S8 [Yates80, Olins81, Mattheakis88, Cerretti88, Merianos04].

Overexpression of rplE suppresses the growth defect of a ΔrsgA mutant [Campbell08].

Citations: [Chang74a, Chang75, Sander75, Kenny75, Bernabeu76, Chen76, Morrison77, Dabbs77, Isono77, Jaskunas77, Jaskunas77a, Branlant77, Fox78, Spierer79, Garrett79, Larrinua79, Newberry80, Shen80, Maimets81, Metspalu82, Cerretti83, Maly83, Tewari83, Suryanarayana83, Traut83, Andrieux84, Siegrist84, Siegrist85, Yusupov86, Goringer86, Kakegawa86, Kashiwagi87, Herold87, Nag87, Lotti89, Redl89, RandolphAnderso89, Dontsova90, Osswald90, Abdurashidova91, Herfurth95, Traut95, Shpanchenko96, Dey98, Agrawal04, Soung09, Meng12]

Gene Citations: [Cerretti83]

Locations: cytosol, ribosome

Map Position: [3,444,921 <- 3,445,460] (74.25 centisomes, 267°)
Length: 540 bp / 179 aa

Molecular Weight of Polypeptide: 20.302 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0010833 , CGSC:259 , DIP:DIP-35914N , EchoBASE:EB0861 , EcoGene:EG10868 , EcoliWiki:b3308 , Mint:MINT-1238892 , OU-Microarray:b3308 , PortEco:rplE , PR:PRO_000023813 , Pride:P62399 , Protein Model Portal:P62399 , RefSeq:NP_417767 , RegulonDB:EG10868 , SMR:P62399 , String:511145.b3308 , UniProt:P62399

Relationship Links: InterPro:IN-FAMILY:IPR002132 , InterPro:IN-FAMILY:IPR020929 , InterPro:IN-FAMILY:IPR020930 , InterPro:IN-FAMILY:IPR022803 , Panther:IN-FAMILY:PTHR11994 , PDB:Structure:1ML5 , PDB:Structure:1P85 , PDB:Structure:1P86 , PDB:Structure:1VS6 , PDB:Structure:1VS8 , PDB:Structure:1VT2 , PDB:Structure:2AW4 , PDB:Structure:2AWB , PDB:Structure:2GYA , PDB:Structure:2GYC , PDB:Structure:2I2T , PDB:Structure:2I2V , PDB:Structure:2J28 , PDB:Structure:2QAM , PDB:Structure:2QAO , PDB:Structure:2QBA , PDB:Structure:2QBC , PDB:Structure:2QBE , PDB:Structure:2QBG , PDB:Structure:2QBI , PDB:Structure:2QBK , PDB:Structure:2QOV , PDB:Structure:2QOX , PDB:Structure:2QOZ , PDB:Structure:2QP1 , PDB:Structure:2RDO , PDB:Structure:2VHM , PDB:Structure:2VHN , PDB:Structure:2WWQ , PDB:Structure:2Z4L , PDB:Structure:2Z4N , PDB:Structure:3BBX , PDB:Structure:3DF2 , PDB:Structure:3DF4 , PDB:Structure:3E1B , PDB:Structure:3E1D , PDB:Structure:3FIK , PDB:Structure:3I1N , PDB:Structure:3I1P , PDB:Structure:3I1R , PDB:Structure:3I1T , PDB:Structure:3I20 , PDB:Structure:3I22 , PDB:Structure:3IZT , PDB:Structure:3IZU , PDB:Structure:3J4X , PDB:Structure:3J01 , PDB:Structure:3J0T , PDB:Structure:3J0W , PDB:Structure:3J0Y , PDB:Structure:3J11 , PDB:Structure:3J12 , PDB:Structure:3J14 , PDB:Structure:3J19 , PDB:Structure:3J37 , PDB:Structure:3J50 , PDB:Structure:3J51 , PDB:Structure:3J52 , PDB:Structure:3J54 , PDB:Structure:3J56 , PDB:Structure:3J58 , PDB:Structure:3J5A , PDB:Structure:3J5C , PDB:Structure:3J5E , PDB:Structure:3J5G , PDB:Structure:3J5I , PDB:Structure:3J5K , PDB:Structure:3J5L , PDB:Structure:3J5O , PDB:Structure:3J5S , PDB:Structure:3J5U , PDB:Structure:3J5W , PDB:Structure:3J7Z , PDB:Structure:3KCR , PDB:Structure:3OAS , PDB:Structure:3OAT , PDB:Structure:3OFC , PDB:Structure:3OFD , PDB:Structure:3OFQ , PDB:Structure:3OFR , PDB:Structure:3OFZ , PDB:Structure:3OG0 , PDB:Structure:3ORB , PDB:Structure:3R8S , PDB:Structure:3R8T , PDB:Structure:3SGF , PDB:Structure:3UOS , PDB:Structure:4CSU , PDB:Structure:4GAR , PDB:Structure:4GAU , PDB:Structure:4KIX , PDB:Structure:4KIZ , PDB:Structure:4KJ1 , PDB:Structure:4KJ3 , PDB:Structure:4KJ5 , PDB:Structure:4KJ7 , PDB:Structure:4KJ9 , PDB:Structure:4KJB , PDB:Structure:4PEB , PDB:Structure:4PEC , PDB:Structure:4TOM , PDB:Structure:4TOO , PDB:Structure:4TOV , PDB:Structure:4TOX , PDB:Structure:4TP1 , PDB:Structure:4TP3 , PDB:Structure:4TP5 , PDB:Structure:4TP7 , PDB:Structure:4TP9 , PDB:Structure:4TPB , PDB:Structure:4TPD , PDB:Structure:4TPF , PDB:Structure:4WAP , PDB:Structure:4WAR , Pfam:IN-FAMILY:PF00281 , Pfam:IN-FAMILY:PF00673 , Prosite:IN-FAMILY:PS00358

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Genetic Regulation Schematic: ?

Genetic regulation schematic for rplE

GO Terms:

Biological Process: GO:0000027 - ribosomal large subunit assembly Inferred from experiment [Korepanov12]
GO:0006412 - translation Inferred by computational analysis [GOA06, GOA01a]
Molecular Function: GO:0008097 - 5S rRNA binding Inferred from experiment [Spierer78]
GO:0000049 - tRNA binding Inferred by computational analysis [UniProtGOA11a, GOA06]
GO:0003723 - RNA binding Inferred by computational analysis [UniProtGOA11a]
GO:0003735 - structural constituent of ribosome Inferred by computational analysis [GOA01a]
GO:0019843 - rRNA binding Inferred by computational analysis [UniProtGOA11a, GOA06]
Cellular Component: GO:0005829 - cytosol Inferred from experiment Inferred by computational analysis [DiazMejia09, Ishihama08, Zhang07]
GO:0022625 - cytosolic large ribosomal subunit Inferred from experiment [Hindennach71]
GO:0005622 - intracellular Inferred by computational analysis [GOA01a]
GO:0005840 - ribosome Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0030529 - ribonucleoprotein complex Inferred by computational analysis [UniProtGOA11a]

MultiFun Terms: cell structure ribosomes
information transfer protein related ribosomal proteins
information transfer protein related translation

Essentiality data for rplE knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox No 37 Aerobic 7   No [Baba06, Comment 2]

Credits:
Last-Curated ? 31-Mar-2015 by Keseler I , SRI International


Subunit of: 50S ribosomal subunit

Synonyms: ribosome, large subunit

Subunit composition of 50S ribosomal subunit = [RrlA][RrfA][RplA][RplB][RplC][RplD][RplE][RplF][(RplJ)([RplL]2)2][RplI][RplK][RplM][RplN][RplO][RplP][RplQ][RplR][RplS][RplT][RplU][RplV][RplW][RplX][RplY][RpmA][RpmB][RpmC][RpmD][RpmE][RpmF][RpmG][RpmH][RpmI][RpmJ]
         23S ribosomal RNA (rrlA) = RrlA (extended summary available)
         5S ribosomal RNA (rrfA) = RrfA (extended summary available)
         50S ribosomal subunit protein L1 = RplA (extended summary available)
         50S ribosomal subunit protein L2 = RplB (summary available)
         50S ribosomal subunit protein L3 = RplC (summary available)
         50S ribosomal subunit protein L4 = RplD (extended summary available)
         50S ribosomal subunit protein L5 = RplE (extended summary available)
         50S ribosomal subunit protein L6 = RplF (summary available)
         50S ribosomal protein complex L8 = (RplJ)([RplL]2)2 (summary available)
                 50S ribosomal subunit protein L10 = RplJ (extended summary available)
                 50S ribosomal subunit protein L7/L12 dimer = (RplL)2
                         50S ribosomal subunit protein L12 = RplL
         50S ribosomal subunit protein L9 = RplI (extended summary available)
         50S ribosomal subunit protein L11 = RplK (extended summary available)
         50S ribosomal subunit protein L13 = RplM (extended summary available)
         50S ribosomal subunit protein L14 = RplN (extended summary available)
         50S ribosomal subunit protein L15 = RplO (summary available)
         50S ribosomal subunit protein L16 = RplP (extended summary available)
         50S ribosomal subunit protein L17 = RplQ (summary available)
         50S ribosomal subunit protein L18 = RplR (extended summary available)
         50S ribosomal subunit protein L19 = RplS (extended summary available)
         50S ribosomal subunit protein L20 = RplT (extended summary available)
         50S ribosomal subunit protein L21 = RplU (summary available)
         50S ribosomal subunit protein L22 = RplV (extended summary available)
         50S ribosomal subunit protein L23 = RplW (extended summary available)
         50S ribosomal subunit protein L24 = RplX (summary available)
         50S ribosomal subunit protein L25 = RplY (extended summary available)
         50S ribosomal subunit protein L27 = RpmA (extended summary available)
         50S ribosomal subunit protein L28 = RpmB (summary available)
         50S ribosomal subunit protein L29 = RpmC (summary available)
         50S ribosomal subunit protein L30 = RpmD (summary available)
         50S ribosomal subunit protein L31 = RpmE (summary available)
         50S ribosomal subunit protein L32 = RpmF (summary available)
         50S ribosomal subunit protein L33 = RpmG (summary available)
         50S ribosomal subunit protein L34 = RpmH (summary available)
         50S ribosomal subunit protein L35 = RpmI (summary available)
         50S ribosomal subunit protein L36 = RpmJ (summary available)

Component of: ribosome (summary available)

Relationship Links: PDB:Structure:1P85 , PDB:Structure:1P86 , PDB:Structure:2AW4 , PDB:Structure:2AWB

Credits:
Created 02-Jun-2006 by Keseler I , SRI International


Subunit of: ribosome

Subunit composition of ribosome = [(RrsA)(RpsA)(RpsB)(RpsC)(RpsD)(RpsE)(RpsF)(RpsG)(RpsH)(RpsI)(RpsJ)(RpsK)(RpsL)(RpsM)(RpsN)(RpsO)(RpsP)(RpsQ)(RpsR)(RpsS)(RpsT)(RpsU)(Sra)][(RrlA)(RrfA)(RplA)(RplB)(RplC)(RplD)(RplE)(RplF)([RplJ][(RplL)2]2)(RplI)(RplK)(RplM)(RplN)(RplO)(RplP)(RplQ)(RplR)(RplS)(RplT)(RplU)(RplV)(RplW)(RplX)(RplY)(RpmA)(RpmB)(RpmC)(RpmD)(RpmE)(RpmF)(RpmG)(RpmH)(RpmI)(RpmJ)]
         30S ribosomal subunit = (RrsA)(RpsA)(RpsB)(RpsC)(RpsD)(RpsE)(RpsF)(RpsG)(RpsH)(RpsI)(RpsJ)(RpsK)(RpsL)(RpsM)(RpsN)(RpsO)(RpsP)(RpsQ)(RpsR)(RpsS)(RpsT)(RpsU)(Sra) (summary available)
                 16S ribosomal RNA (rrsA) = RrsA (extended summary available)
                 30S ribosomal subunit protein S1 = RpsA (extended summary available)
                 30S ribosomal subunit protein S2 = RpsB (summary available)
                 30S ribosomal subunit protein S3 = RpsC (summary available)
                 30S ribosomal subunit protein S4 = RpsD (extended summary available)
                 30S ribosomal subunit protein S5 = RpsE (extended summary available)
                 30S ribosomal subunit protein S6 = RpsF (extended summary available)
                 30S ribosomal subunit protein S7 = RpsG (extended summary available)
                 30S ribosomal subunit protein S8 = RpsH (extended summary available)
                 30S ribosomal subunit protein S9 = RpsI (extended summary available)
                 30S ribosomal subunit protein S10 = RpsJ (extended summary available)
                 30S ribosomal subunit protein S11 = RpsK (summary available)
                 30S ribosomal subunit protein S12 = RpsL (extended summary available)
                 30S ribosomal subunit protein S13 = RpsM (extended summary available)
                 30S ribosomal subunit protein S14 = RpsN (summary available)
                 30S ribosomal subunit protein S15 = RpsO (extended summary available)
                 30S ribosomal subunit protein S16 = RpsP (summary available)
                 30S ribosomal subunit protein S17 = RpsQ (summary available)
                 30S ribosomal subunit protein S18 = RpsR (extended summary available)
                 30S ribosomal subunit protein S19 = RpsS (summary available)
                 30S ribosomal subunit protein S20 = RpsT (extended summary available)
                 30S ribosomal subunit protein S21 = RpsU (summary available)
                 30S ribosomal subunit protein S22 = Sra (summary available)
         50S ribosomal subunit = (RrlA)(RrfA)(RplA)(RplB)(RplC)(RplD)(RplE)(RplF)([RplJ][(RplL)2]2)(RplI)(RplK)(RplM)(RplN)(RplO)(RplP)(RplQ)(RplR)(RplS)(RplT)(RplU)(RplV)(RplW)(RplX)(RplY)(RpmA)(RpmB)(RpmC)(RpmD)(RpmE)(RpmF)(RpmG)(RpmH)(RpmI)(RpmJ)
                 23S ribosomal RNA (rrlA) = RrlA (extended summary available)
                 5S ribosomal RNA (rrfA) = RrfA (extended summary available)
                 50S ribosomal subunit protein L1 = RplA (extended summary available)
                 50S ribosomal subunit protein L2 = RplB (summary available)
                 50S ribosomal subunit protein L3 = RplC (summary available)
                 50S ribosomal subunit protein L4 = RplD (extended summary available)
                 50S ribosomal subunit protein L5 = RplE (extended summary available)
                 50S ribosomal subunit protein L6 = RplF (summary available)
                 50S ribosomal protein complex L8 = (RplJ)([RplL]2)2 (summary available)
                         50S ribosomal subunit protein L10 = RplJ (extended summary available)
                         50S ribosomal subunit protein L7/L12 dimer = (RplL)2
                                 50S ribosomal subunit protein L12 = RplL
                 50S ribosomal subunit protein L9 = RplI (extended summary available)
                 50S ribosomal subunit protein L11 = RplK (extended summary available)
                 50S ribosomal subunit protein L13 = RplM (extended summary available)
                 50S ribosomal subunit protein L14 = RplN (extended summary available)
                 50S ribosomal subunit protein L15 = RplO (summary available)
                 50S ribosomal subunit protein L16 = RplP (extended summary available)
                 50S ribosomal subunit protein L17 = RplQ (summary available)
                 50S ribosomal subunit protein L18 = RplR (extended summary available)
                 50S ribosomal subunit protein L19 = RplS (extended summary available)
                 50S ribosomal subunit protein L20 = RplT (extended summary available)
                 50S ribosomal subunit protein L21 = RplU (summary available)
                 50S ribosomal subunit protein L22 = RplV (extended summary available)
                 50S ribosomal subunit protein L23 = RplW (extended summary available)
                 50S ribosomal subunit protein L24 = RplX (summary available)
                 50S ribosomal subunit protein L25 = RplY (extended summary available)
                 50S ribosomal subunit protein L27 = RpmA (extended summary available)
                 50S ribosomal subunit protein L28 = RpmB (summary available)
                 50S ribosomal subunit protein L29 = RpmC (summary available)
                 50S ribosomal subunit protein L30 = RpmD (summary available)
                 50S ribosomal subunit protein L31 = RpmE (summary available)
                 50S ribosomal subunit protein L32 = RpmF (summary available)
                 50S ribosomal subunit protein L33 = RpmG (summary available)
                 50S ribosomal subunit protein L34 = RpmH (summary available)
                 50S ribosomal subunit protein L35 = RpmI (summary available)
                 50S ribosomal subunit protein L36 = RpmJ (summary available)

Summary:
The ribosome is a complex machinery that translates the genetic code.

A crystal structure of the E. coli ribosome has been determined at 3.5 Å resolution [Schuwirth05]. Additional crystal structures of the ribosome with tRNA bound in two functionally distinct states reveal how a ratchet-like motion of the small and large subunits contributes to translocation, termination of translation, and ribosome recycling [Zhang09b, Dunkle11].

Approximately eight molecules of Zn2+ are bound to the ribosome; therefore, it appears that a large fraction of intracellular Zn2+ is ribosome-associated [Hensley11].

Selected reviews: [Ramakrishnan02, Yonath05, Ogle05, Kaczanowska07]

Citations: [Kuhlenkoetter11]

Relationship Links: PDB:Structure:3R8N , PDB:Structure:3R8O , PDB:Structure:3R8S , PDB:Structure:3R8T

Credits:
Created 15-Jun-2006 by Keseler I , SRI International


Sequence Features

Protein sequence of 50S ribosomal subunit protein L5 with features indicated

Feature Class Location Citations Comment
Cleavage-of-Initial-Methionine 1
[Chen76, Arnold99, UniProt11, Arnold99]
UniProt: Removed.
Chain 2 -> 179
[UniProt09]
UniProt: 50S ribosomal protein L5;
N6-acetyllysine-Modification 3
[Zhang09, UniProt15]
UniProt: N6-acetyllysine.
Acetylation-Modification 9
[Yu08]
 
Sequence-Conflict 94
[Chen76, UniProt10]
UniProt: (in Ref. 4; AA sequence);
Acetylation-Modification 120
[Yu08]
 
Sequence-Conflict 127
[Chen76, UniProt10]
UniProt: (in Ref. 4; AA sequence);


Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram

Notes:

History:
10/20/97 Gene b3308 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG10868; confirmed by SwissProt match.


References

Abdurashidova90: Abdurashidova GG, Tsvetkova EA, Budowsky EI (1990). "Determination of tRNA nucleotide residues directly interacting with proteins in the post- and pretranslocated ribosomal complexes." FEBS Lett 269(2);398-401. PMID: 2205514

Abdurashidova91: Abdurashidova GG, Tsvetkova EA, Budowsky EI (1991). "Direct tRNA-protein interactions in ribosomal complexes." Nucleic Acids Res 19(8);1909-15. PMID: 1709494

Agrawal04: Agrawal RK, Sharma MR, Kiel MC, Hirokawa G, Booth TM, Spahn CM, Grassucci RA, Kaji A, Frank J (2004). "Visualization of ribosome-recycling factor on the Escherichia coli 70S ribosome: functional implications." Proc Natl Acad Sci U S A 101(24);8900-5. PMID: 15178758

Andrieux84: Andrieux E, Cozzone AJ (1984). "Conformational changes in bacterial polysomes induced by amino acid starvation." Int J Biochem 16(1);113-6. PMID: 6365649

Arnold99: Arnold RJ, Reilly JP (1999). "Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry." Anal Biochem 269(1);105-12. PMID: 10094780

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Bernabeu76: Bernabeu C, Vazquez D, Ballesta JP (1976). "Activities of protein-deficient particles derived from 50-S ribosomal subunits by NH4Cl/ethanol treatment." Eur J Biochem 69(1);233-41. PMID: 791646

Bock13: Bock LV, Blau C, Schroder GF, Davydov II, Fischer N, Stark H, Rodnina MV, Vaiana AC, Grubmuller H (2013). "Energy barriers and driving forces in tRNA translocation through the ribosome." Nat Struct Mol Biol 20(12);1390-6. PMID: 24186064

Branlant76: Branlant C, Krol A, Sriwidada J, Brimacombe R (1976). "RNA sequences associated with proteins L1, L9, and L5, L18, L25, in ribonucleoprotein fragments isolated from the 50-S subunit of Escherichia coli ribosomes." Eur J Biochem 70(2);483-92. PMID: 827440

Branlant77: Branlant C, Widada JS, Krol A, Ebel JP (1977). "Studies on the primary structure of the ribosomal 23S RNA of Escherichia coli: II. A characterisation and an alignment of 24 sections spanning the entire molecule and its application to the localisation of specific fragments." Nucleic Acids Res 4(12);4323-45. PMID: 414209

Campbell08: Campbell TL, Brown ED (2008). "Genetic interaction screens with ordered overexpression and deletion clonesets implicate the Escherichia coli GTPase YjeQ in late ribosome biogenesis." J Bacteriol 190(7);2537-45. PMID: 18223068

Cerretti83: Cerretti DP, Dean D, Davis GR, Bedwell DM, Nomura M (1983). "The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene." Nucleic Acids Res 1983;11(9);2599-616. PMID: 6222285

Cerretti88: Cerretti DP, Mattheakis LC, Kearney KR, Vu L, Nomura M (1988). "Translational regulation of the spc operon in Escherichia coli. Identification and structural analysis of the target site for S8 repressor protein." J Mol Biol 204(2);309-29. PMID: 2464692

Chaires82: Chaires JB, Hawley DA, Wahba AJ (1982). "Chain initiation factor 3 crosslinks to E. coli 30S and 50S ribosomal subunits and alters the UV absorbance spectrum of 70S ribosomes." Nucleic Acids Res 10(18);5681-93. PMID: 6755396

Chang74a: Chang FN, Chang CN, Paik WK (1974). "Methylation of ribosomal proteins in Escherichia coli." J Bacteriol 120(2);651-6. PMID: 4616944

Chang75: Chang CN, Chang N (1975). "Methylation of the ribosomal proteins in Escherichia coli. Nature and stoichiometry of the methylated amino acids in 50S ribosomal proteins." Biochemistry 14(3);468-77. PMID: 1089427

Chen76: Chen R, Ehrke G (1976). "The primary structure of the 5 S RNA binding protein L5 of Escherichia coli ribosomes." FEBS Lett 69(1);240-5. PMID: 791672

ChenSchmeisser77: Chen-Schmeisser U, Garrett RA (1977). "A new method for the isolation of a 5 S RNA complex with proteins L5, L18 and L25 from Escherichia coli ribosomes." FEBS Lett 74(2);287-91. PMID: 321249

Dabbs77: Dabbs ER (1977). "A spectinomycin dependent mutant of Escherichia coli." Mol Gen Genet 151(3);261-7. PMID: 141001

Dey98: Dey D, Bochkariov DE, Jokhadze GG, Traut RR (1998). "Cross-linking of selected residues in the N- and C-terminal domains of Escherichia coli protein L7/L12 to other ribosomal proteins and the effect of elongation factor Tu." J Biol Chem 273(3);1670-6. PMID: 9430711

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Dontsova90: Dontsova OA, Efimov AV, Kopylov AM (1990). "[The 5S rRNA-protein complex of Escherichia coli studied by carbodiimide modification]." Nauchnye Doki Vyss Shkoly Biol Nauki (2);22-30. PMID: 1693861

Dunkle11: Dunkle JA, Wang L, Feldman MB, Pulk A, Chen VB, Kapral GJ, Noeske J, Richardson JS, Blanchard SC, Cate JH (2011). "Structures of the bacterial ribosome in classical and hybrid states of tRNA binding." Science 332(6032);981-4. PMID: 21596992

Fox78: Fox JW, Wong KP (1978). "Changes in the conformation and stability of 5 S RNA upon the binding of ribosomal proteins." J Biol Chem 253(1);18-20. PMID: 338605

Gao03: Gao H, Sengupta J, Valle M, Korostelev A, Eswar N, Stagg SM, Van Roey P, Agrawal RK, Harvey SC, Sali A, Chapman MS, Frank J (2003). "Study of the structural dynamics of the E coli 70S ribosome using real-space refinement." Cell 113(6);789-801. PMID: 12809609

Garrett79: Garrett RA, Noller HF (1979). "Structures of complexes of 5S RNA with ribosomal proteins L5, L18 and L25 from Escherichia coli: identification of kethoxal-reactive sites on the 5S RNA." J Mol Biol 132(4);637-48. PMID: 393829

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

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Other References Related to Gene Regulation

Dean81: Dean D, Yates JL, Nomura M (1981). "Escherichia coli ribosomal protein S8 feedback regulates part of spc operon." Nature 289(5793);89-91. PMID: 6450328

Lemke11: Lemke JJ, Sanchez-Vazquez P, Burgos HL, Hedberg G, Ross W, Gourse RL (2011). "Direct regulation of Escherichia coli ribosomal protein promoters by the transcription factors ppGpp and DksA." Proc Natl Acad Sci U S A 108(14);5712-7. PMID: 21402902

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Post78: Post LE, Arfsten AE, Reusser F, Nomura M (1978). "DNA sequences of promoter regions for the str and spc ribosomal protein operons in E. coli." Cell 15(1);215-29. PMID: 151587

Post78a: Post LE, Arfsten AE, Nomura M, Jaskunas SR (1978). "Isolation and characterization of a promoter mutant in the str ribosomal protein operon in E. coli." Cell 1978;15(1);231-6. PMID: 359165


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