Escherichia coli K-12 substr. MG1655 Polypeptide: NAD-binding component of TrK potassium transporter

Gene: trkA Accession Numbers: EG11019 (EcoCyc), b3290, ECK3276

Regulation Summary Diagram: ?

Regulation summary diagram for trkA

trkA mutants were identified in a kdp background as requiring significantly elevated levels of K+ for growth [Epstein71]. trkA encodes part of a K+ transport system [Epstein71]. The TrkA system is constitutive with a Km of 1.5 mM [Rhoads76]. K+ uptake by TrkA is both ATP-dependent and protonmotive force (pmf)-driven [Rhoads77], though K+ exchange is not dependent upon the pmf [Rhoads78]. TrkA is inhibited by high intracellular K+ [Rhoads78] and by low pH [Bakker83]. Efflux of K+ by TrkA depends upon the intracellular concentration of K+ [Meury81]. trkE, trkG, and trkH mutations reduced or prevented binding of TrkA to the membrane [Bossemeyer89]. The TrkG and TrkH membrane proteins were identified as two different but nearly equivalent systems of K+ uptake, each requiring TrkA and TrkE [Dosch91]. UV crosslinking studies showed binding of TrkA to NAD+ but not to ATP [Schlosser93]. The TrkA pump may be involved in regulation of pH in anaerobically growing cells at alkaline pH [Trchounian98]. The F0F1 ATPase is dependent on TrkA when cells are grown anaerobically on glucose at alkaline pH [Bagramyan03a].

Locations: inner membrane

Map Position: [3,434,540 -> 3,435,916] (74.03 centisomes, 266°)
Length: 1377 bp / 458 aa

Molecular Weight of Polypeptide: 50.368 kD (from nucleotide sequence), 53 kD (experimental) [Hamann87 ]

Unification Links: ASAP:ABE-0010787 , CGSC:84 , DIP:DIP-35971N , EchoBASE:EB1012 , EcoGene:EG11019 , EcoliWiki:b3290 , ModBase:P0AGI8 , OU-Microarray:b3290 , PortEco:trkA , PR:PRO_000024112 , Pride:P0AGI8 , Protein Model Portal:P0AGI8 , RefSeq:NP_417748 , RegulonDB:EG11019 , SMR:P0AGI8 , String:511145.b3290 , UniProt:P0AGI8

Relationship Links: InterPro:IN-FAMILY:IPR003148 , InterPro:IN-FAMILY:IPR006036 , InterPro:IN-FAMILY:IPR006037 , InterPro:IN-FAMILY:IPR016040 , Pfam:IN-FAMILY:PF02080 , Pfam:IN-FAMILY:PF02254 , Prints:IN-FAMILY:PR00335 , Prosite:IN-FAMILY:PS51201 , Prosite:IN-FAMILY:PS51202

In Paralogous Gene Group: 515 (2 members)

GO Terms:

Biological Process: GO:0006813 - potassium ion transport Inferred from experiment Inferred by computational analysis [UniProtGOA11, GOA01, Meury81]
GO:0098655 - cation transmembrane transport Inferred from experiment Inferred by computational analysis [GOA01, Meury81]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
GO:0006811 - ion transport Inferred by computational analysis [UniProtGOA11]
GO:0044550 - secondary metabolite biosynthetic process Inferred by computational analysis [Gaudet10]
GO:0055114 - oxidation-reduction process Inferred by computational analysis [Gaudet10]
GO:0071805 - potassium ion transmembrane transport Inferred by computational analysis [GOA01]
Molecular Function: GO:0008324 - cation transmembrane transporter activity Inferred from experiment Inferred by computational analysis [GOA01, Meury81]
GO:0004497 - monooxygenase activity Inferred by computational analysis [Gaudet10]
GO:0015079 - potassium ion transmembrane transporter activity Inferred by computational analysis [GOA01]
Cellular Component: GO:0016020 - membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, Bossemeyer89]
GO:0005886 - plasma membrane Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, GOA01, Bossemeyer89]

MultiFun Terms: cell structure membrane
transport Electrochemical potential driven transporters Porters (Uni-, Sym- and Antiporters)

Essentiality data for trkA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Sequence Features

Protein sequence of NAD-binding component of TrK potassium transporter with features indicated

Feature Class Location Attached Group Citations Comment
Conserved-Region 2 -> 131  
UniProt: RCK N-terminal 1;
Nucleotide-Phosphate-Binding-Region 7 -> 11 NAD 1
UniProt: NAD 1; Non-Experimental Qualifier: by similarity.
Amino-Acid-Sites-That-Bind 30  
UniProt: NAD 1; Non-Experimental Qualifier: by similarity.
Nucleotide-Phosphate-Binding-Region 73 -> 74 NAD 1
UniProt: NAD 1; Non-Experimental Qualifier: by similarity.
Amino-Acid-Sites-That-Bind 98  
UniProt: NAD 1; shared with dimeric partner; Non-Experimental Qualifier: by similarity.
Conserved-Region 143 -> 227  
UniProt: RCK C-terminal 1;
Nucleotide-Phosphate-Binding-Region 234 -> 262  
UniProt: NAD; Non-Experimental Qualifier: potential;
Conserved-Region 234 -> 356  
UniProt: RCK N-terminal 2;
Conserved-Region 368 -> 453  
UniProt: RCK C-terminal 2;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


10/20/97 Gene b3290 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11019; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Bagramyan03a: Bagramyan K, Mnatsakanyan N, Trchounian A (2003). "Formate increases the F0F1-ATPase activity in Escherichia coli growing on glucose under anaerobic conditions at slightly alkaline pH." Biochem Biophys Res Commun 306(2);361-5. PMID: 12804571

Bakker83: Bakker EP, Mangerich WE (1983). "The effects of weak acids on potassium uptake by Escherichia coli K-12 inhibition by low cytoplasmic pH." Biochim Biophys Acta 730(2);379-86. PMID: 6405784

Bossemeyer89: Bossemeyer D, Borchard A, Dosch DC, Helmer GC, Epstein W, Booth IR, Bakker EP (1989). "K+-transport protein TrkA of Escherichia coli is a peripheral membrane protein that requires other trk gene products for attachment to the cytoplasmic membrane." J Biol Chem 1989;264(28);16403-10. PMID: 2674131

Dosch91: Dosch DC, Helmer GL, Sutton SH, Salvacion FF, Epstein W (1991). "Genetic analysis of potassium transport loci in Escherichia coli: evidence for three constitutive systems mediating uptake potassium." J Bacteriol 1991;173(2);687-96. PMID: 1987159

Epstein71: Epstein W, Kim BS (1971). "Potassium transport loci in Escherichia coli K-12." J Bacteriol 108(2);639-44. PMID: 4942756

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gaudet10: Gaudet P, Livstone M, Thomas P (2010). "Annotation inferences using phylogenetic trees." PMID: 19578431

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Hamann87: Hamann A, Bossemeyer D, Bakker EP (1987). "Physical mapping of the K+ transport trkA gene of Escherichia coli and overproduction of the TrkA protein." J Bacteriol 169(7);3138-45. PMID: 3298214

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Meury81: Meury J, Kepes A (1981). "The regulation of potassium fluxes for the adjustment and maintenance of potassium levels in Escherichia coli." Eur J Biochem 119(1);165-70. PMID: 7042336

Rhoads76: Rhoads DB, Waters FB, Epstein W (1976). "Cation transport in Escherichia coli. VIII. Potassium transport mutants." J Gen Physiol 67(3);325-41. PMID: 4578

Rhoads77: Rhoads DB, Epstein W (1977). "Energy coupling to net K+ transport in Escherichia coli K-12." J Biol Chem 252(4);1394-401. PMID: 320207

Rhoads78: Rhoads DB, Epstein W (1978). "Cation transport in Escherichia coli. IX. Regulation of K transport." J Gen Physiol 72(3);283-95. PMID: 359759

Schlosser93: Schlosser A, Hamann A, Bossemeyer D, Schneider E, Bakker EP (1993). "NAD+ binding to the Escherichia coli K(+)-uptake protein TrkA and sequence similarity between TrkA and domains of a family of dehydrogenases suggest a role for NAD+ in bacterial transport." Mol Microbiol 1993;9(3);533-43. PMID: 8412700

Trchounian98: Trchounian A, Ohanjayan E, Zakharyan E (1998). "Regulation of intracellular pH and proton-potassium exchange in fermenting Escherichia coli grown anaerobically in alkaline medium." Membr Cell Biol 12(1);67-78. PMID: 9829260

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt13: UniProt Consortium (2013). "UniProt version 2013-08 released on 2013-08-01 00:00:00." Database.

UniProt14: UniProt Consortium (2014). "UniProt version 2014-01 released on 2014-01-01 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Zaslaver06: Zaslaver A, Bren A, Ronen M, Itzkovitz S, Kikoin I, Shavit S, Liebermeister W, Surette MG, Alon U (2006). "A comprehensive library of fluorescent transcriptional reporters for Escherichia coli." Nat Methods 3(8);623-8. PMID: 16862137

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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