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Escherichia coli K-12 substr. MG1655 Transporter: lysine:H+ symporter



Gene: lysP Accession Numbers: EG11337 (EcoCyc), b2156, ECK2149

Synonyms: cadR, LysP lysine APC transporter

Regulation Summary Diagram: ?

Summary:
LysP is one of two principal systems responsible for the uptake of lysine, the other is the ArgT ABC transporter. LysP is specific for lysine while ArgT also transports arginine and ornithine. Inactivation of the lysP gene decreased lysine accumulation and could be complemented by the cloned lysP gene [Steffes92]. LysP-mediated lysine transport is increased by growth in acidic media, anaerobiosis or high concentrations of lysine [Steffes92].

Topological analysis using B-lactamase fusions has shown that LysE consists of 12 transmembrane segments [Ellis95]. LysE is a member of the APC superfamily of amino acid transporters and probably functions as a lysine/proton symporter.

LysP has a role in lysine dependent regulation of the Cad system which is involved in E. coli's adaption to acidic environments [Bearson97]. Overexpression of lysP inhibits expression of cadBA [Neely94]. cadC mutants containing various single amino acid replacements are resistant to this effect [Tetsch08]. LysP interacts directly with CadC in proteoliposomes [Tetsch08]. LysP and CadC interact most strongly at pH7.6 and at lysine concentrations of 1 µM or less, that is, under non-Cad-inducing conditions. This interaction is attenuated under Cad-inducing conditions (low pH, increased lysine levels). The LysP-CadC interaction is mediated via trans-membrane and periplasmic contacts [Rauschmeier14]. Oligomerization of LysP is induced at low pH [Rauschmeier14]. LysP is a 'trigger transporter' [Tetsch09]- a protein that combines regulation and transport function within the one polypeptide.

Locations: inner membrane

Map Position: [2,245,085 <- 2,246,554] (48.39 centisomes)
Length: 1470 bp / 489 aa

Molecular Weight of Polypeptide: 53.576 kD (from nucleotide sequence), 40.0 kD (experimental) [Steffes92 ]

Unification Links: ASAP:ABE-0007131 , CGSC:942 , EchoBASE:EB1313 , EcoGene:EG11337 , EcoliWiki:b2156 , OU-Microarray:b2156 , PortEco:lysP , PR:PRO_000023139 , Protein Model Portal:P25737 , RefSeq:NP_416661 , RegulonDB:EG11337 , SMR:P25737 , String:511145.b2156 , UniProt:P25737

Relationship Links: InterPro:IN-FAMILY:IPR002293 , InterPro:IN-FAMILY:IPR004840 , InterPro:IN-FAMILY:IPR004841 , Panther:IN-FAMILY:PTHR11785 , Pfam:IN-FAMILY:PF00324 , Prosite:IN-FAMILY:PS00218

In Paralogous Gene Group: 37 (10 members)

Gene-Reaction Schematic: ?

Genetic Regulation Schematic: ?

GO Terms:

Biological Process: GO:0051260 - protein homooligomerization Inferred from experiment [Rauschmeier14]
GO:1902022 - L-lysine transport Inferred from experiment [Steffes92]
GO:0003333 - amino acid transmembrane transport Inferred by computational analysis [GOA01a]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0006865 - amino acid transport Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0055085 - transmembrane transport Inferred by computational analysis [GOA01a]
Molecular Function: GO:0015171 - amino acid transmembrane transporter activity Inferred from experiment Inferred by computational analysis [GOA01a, Steffes92]
GO:0042802 - identical protein binding Inferred from experiment [Rauschmeier14]
GO:0046982 - protein heterodimerization activity Inferred from experiment [Rauschmeier14, Tetsch08]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Zhang07, Daley05]
GO:0005887 - integral component of plasma membrane Inferred from experiment [Ellis95]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]

MultiFun Terms: cell structure membrane
metabolism biosynthesis of building blocks amino acids lysine
transport Electrochemical potential driven transporters Porters (Uni-, Sym- and Antiporters)

Essentiality data for lysP knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Enzymatic reaction of: lysine:H+ symporter

Synonyms: Transport of L-lysine

Kinetic Parameters:

Substrate
Km (μM)
Citations
L-lysine
2.5
[Rauschmeier14]


Sequence Features

Feature Class Location Citations Comment
Cleavage-of-Initial-Methionine 1
[Ellis95, UniProt11]
UniProt: Removed.
Chain 2 -> 489
[UniProt09]
UniProt: Lysine-specific permease;
Transmembrane-Region 22 -> 42
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: probable;
Transmembrane-Region 56 -> 76
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: probable;
Transmembrane-Region 105 -> 125
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: probable;
Sequence-Conflict 122
[Richterich93, UniProt10]
Alternate sequence: S → N; UniProt: (in Ref. 3; AAA60532);
Transmembrane-Region 131 -> 149
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: probable;
Transmembrane-Region 161 -> 181
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: probable;
Transmembrane-Region 200 -> 218
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: probable;
Transmembrane-Region 245 -> 264
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: probable;
Transmembrane-Region 284 -> 304
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: probable;
Transmembrane-Region 348 -> 365
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: probable;
Transmembrane-Region 372 -> 392
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: probable;
Transmembrane-Region 416 -> 433
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: probable;
Transmembrane-Region 450 -> 470
[UniProt10a]
UniProt: Helical;; Non-Experimental Qualifier: probable;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b2156 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11337; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Bearson97: Bearson S, Bearson B, Foster JW (1997). "Acid stress responses in enterobacteria." FEMS Microbiol Lett 147(2);173-80. PMID: 9119190

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Ellis95: Ellis J, Carlin A, Steffes C, Wu J, Liu J, Rosen BP (1995). "Topological analysis of the lysine-specific permease of Escherichia coli." Microbiology 1995;141 ( Pt 8);1927-35. PMID: 7551055

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Neely94: Neely MN, Dell CL, Olson ER (1994). "Roles of LysP and CadC in mediating the lysine requirement for acid induction of the Escherichia coli cad operon." J Bacteriol 176(11);3278-85. PMID: 8195083

Rauschmeier14: Rauschmeier M, Schuppel V, Tetsch L, Jung K (2014). "New insights into the interplay between the lysine transporter LysP and the pH sensor CadC in Escherichia coli." J Mol Biol 426(1);215-29. PMID: 24056175

Richterich93: Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K., Church G.M. (1993). "Automated multiplex sequencing of the E.coli genome." Data submission to EMBL/GenBank/DDBJ databases on 1993-10.

Steffes92: Steffes C, Ellis J, Wu J, Rosen BP (1992). "The lysP gene encodes the lysine-specific permease." J Bacteriol 1992;174(10);3242-9. PMID: 1315732

Tetsch08: Tetsch L, Koller C, Haneburger I, Jung K (2008). "The membrane-integrated transcriptional activator CadC of Escherichia coli senses lysine indirectly via the interaction with the lysine permease LysP." Mol Microbiol 67(3);570-83. PMID: 18086202

Tetsch09: Tetsch L, Jung K (2009). "The regulatory interplay between membrane-integrated sensors and transport proteins in bacteria." Mol Microbiol 73(6);982-91. PMID: 19708919

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt11: UniProt Consortium (2011). "UniProt version 2011-06 released on 2011-06-30 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Zhang07: Zhang N, Chen R, Young N, Wishart D, Winter P, Weiner JH, Li L (2007). "Comparison of SDS- and methanol-assisted protein solubilization and digestion methods for Escherichia coli membrane proteome analysis by 2-D LC-MS/MS." Proteomics 7(4);484-93. PMID: 17309111

Other References Related to Gene Regulation

Goss08: Goss TJ (2008). "The ArgP protein stimulates the Klebsiella pneumoniae gdhA promoter in a lysine-sensitive manner." J Bacteriol 190(12);4351-9. PMID: 18424527

Lee97a: Lee Y, Lee H, Yim J, Hwang D (1997). "The binding of two dimers of IciA protein to the dnaA promoter 1P element enhances the binding of RNA polymerase to the dnaA promoter 1P." Nucleic Acids Res 1997;25(17);3486-9. PMID: 9254708

Marbaniang11: Marbaniang CN, Gowrishankar J (2011). "Role of ArgP (IciA) in Lysine-Mediated Repression in Escherichia coli." J Bacteriol 193(21);5985-96. PMID: 21890697

Neely96: Neely MN, Olson ER (1996). "Kinetics of expression of the Escherichia coli cad operon as a function of pH and lysine." J Bacteriol 178(18);5522-8. PMID: 8808945

Ruiz11: Ruiz J, Haneburger I, Jung K (2011). "Identification of ArgP and Lrp as transcriptional regulators of lysP, the gene encoding the specific lysine permease of Escherichia coli." J Bacteriol 193(10);2536-48. PMID: 21441513


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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sat Nov 22, 2014, BIOCYC14B.