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Escherichia coli K-12 substr. MG1655 Transporter: K+ transporter Kup



Gene: kup Accession Numbers: EG11541 (EcoCyc), b3747, ECK3741

Synonyms: trkD

Regulation Summary Diagram: ?

Summary:
Kup (TrkD) is involved in potassium ion uptake under hyper-osmotic stress at a low pH [Trchounian99]. The Kup protein is composed of two domains--an integral membrane domain that has 12 putative trans-membrane spanners and a hydrophilic C-terminal domain. Both N and C termini of the protein are located at the cytoplasmic side of the cell membrane and deletion of most of the hydrophilic domain reduced, but did not abolish Kup transport activity [Schleyer93]. Kup has been shown to also transport cations such as Cs+ and Rb+ [Bossemeyer89]. While it is assumed to be a secondary transporter, and its uptake is blocked by protonophores such as CCCP, the energy coupling mechanism has not been defined. Other possible transporters for potassium ion uptake in E. coli include Kdp and TrkA [Trchounian99].

Locations: inner membrane

Map Position: [3,929,339 -> 3,931,207] (84.69 centisomes)
Length: 1869 bp / 622 aa

Molecular Weight of Polypeptide: 69.294 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0012251 , CGSC:81 , EchoBASE:EB1503 , EcoGene:EG11541 , EcoliWiki:b3747 , OU-Microarray:b3747 , PortEco:kup , PR:PRO_000023068 , Pride:P63183 , Protein Model Portal:P63183 , RefSeq:YP_026244 , RegulonDB:EG11541 , String:511145.b3747 , UniProt:P63183

Relationship Links: InterPro:IN-FAMILY:IPR003855 , InterPro:IN-FAMILY:IPR023051 , Pfam:IN-FAMILY:PF02705

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0006813 - potassium ion transport Inferred from experiment Inferred by computational analysis [UniProtGOA11, GOA06, Trchounian99]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
GO:0006811 - ion transport Inferred by computational analysis [UniProtGOA11]
GO:0071805 - potassium ion transmembrane transport Inferred by computational analysis [GOA01]
Molecular Function: GO:0015079 - potassium ion transmembrane transporter activity Inferred from experiment Inferred by computational analysis [GOA06, GOA01, Schleyer93]
GO:0015293 - symporter activity Inferred by computational analysis [UniProtGOA11, GOA06]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, GOA06, DiazMejia09, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11]

MultiFun Terms: cell processes adaptations osmotic pressure
cell structure membrane
transport Electrochemical potential driven transporters Porters (Uni-, Sym- and Antiporters)

Essentiality data for kup knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Enzymatic reaction of: K+ transporter Kup


Sequence Features

Feature Class Location Citations Comment
Transmembrane-Region 9 -> 29
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 49 -> 69
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 103 -> 123
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 137 -> 157
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 165 -> 185
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 213 -> 233
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 247 -> 267
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 276 -> 296
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 337 -> 357
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 363 -> 383
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 396 -> 416
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 419 -> 439
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Units:

Notes:

History:
Suzanne Paley on Thu Oct 21, 2004:
Position updated based on U00096.2 release of genome
10/20/97 Gene b3747 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11541; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Bossemeyer89: Bossemeyer D, Schlosser A, Bakker EP (1989). "Specific cesium transport via the Escherichia coli Kup (TrkD) K+ uptake system." J Bacteriol 1989;171(4);2219-21. PMID: 2649491

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Schleyer93: Schleyer M, Bakker EP (1993). "Nucleotide sequence and 3'-end deletion studies indicate that the K(+)-uptake protein kup from Escherichia coli is composed of a hydrophobic core linked to a large and partially essential hydrophilic C terminus." J Bacteriol 1993;175(21);6925-31. PMID: 8226635

Trchounian99: Trchounian A, Kobayashi H (1999). "Kup is the major K+ uptake system in Escherichia coli upon hyper-osmotic stress at a low pH." FEBS Lett 1999;447(2-3);144-8. PMID: 10214935

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

MendozaVargas09: Mendoza-Vargas A, Olvera L, Olvera M, Grande R, Vega-Alvarado L, Taboada B, Jimenez-Jacinto V, Salgado H, Juarez K, Contreras-Moreira B, Huerta AM, Collado-Vides J, Morett E (2009). "Genome-wide identification of transcription start sites, promoters and transcription factor binding sites in E. coli." PLoS One 4(10);e7526. PMID: 19838305


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Wed Nov 26, 2014, biocyc13.