Escherichia coli K-12 substr. MG1655 Polypeptide: periplasmic binding protein ArtI

Gene: artI Accession Numbers: EG11625 (EcoCyc), b0863, ECK0854

Regulation Summary Diagram: ?

Regulation summary diagram for artI

Component of: predicted ABC transporter ArtPQMI (summary available)

Sequence analysis indicates that ArtI has similarity to the histidine-binding periplasmic protein (HisJ) and lysine/arginine/ornithine binding periplasmic protein (ArgT), both from Salmonella typhimurium. ArtI is also similar to the ArtJ arginine binding periplasmic protein of E. coli. Purified ArtI does not bind any of 20 common amino acids nor does it bind labelled arginine nor purescine [Wissenbach95].

artI is part of the artMQIP operon which is regulated by the ArgR repressor in response to arginine [Caldara06, Caldara07]. It is assumed to form an ABC transporter of unknown specificity with the ArtMQP membrane components [Wissenbach95].

Gene Citations: [Wissenbach93]

Locations: periplasmic space

Map Position: [901,480 <- 902,211] (19.43 centisomes, 70°)
Length: 732 bp / 243 aa

Molecular Weight of Polypeptide: 26.93 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0002937 , CGSC:31674 , EchoBASE:EB1582 , EcoGene:EG11625 , EcoliWiki:b0863 , ModBase:P30859 , OU-Microarray:b0863 , PortEco:artI , Pride:P30859 , Protein Model Portal:P30859 , RefSeq:NP_415384 , RegulonDB:EG11625 , SMR:P30859 , String:511145.b0863 , UniProt:P30859

Relationship Links: InterPro:IN-FAMILY:IPR001638 , InterPro:IN-FAMILY:IPR005768 , InterPro:IN-FAMILY:IPR018313 , Pfam:IN-FAMILY:PF00497 , Prosite:IN-FAMILY:PS01039 , Smart:IN-FAMILY:SM00062

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

Genetic Regulation Schematic: ?

Genetic regulation schematic for artI

GO Terms:

Biological Process: GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0006865 - amino acid transport Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0005215 - transporter activity Inferred by computational analysis [GOA01a]
Cellular Component: GO:0030288 - outer membrane-bounded periplasmic space Inferred from experiment Inferred by computational analysis [GOA01a, DiazMejia09, LopezCampistrou05, Wissenbach95, Han14]
GO:0042597 - periplasmic space Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, Wissenbach95]

MultiFun Terms: metabolism biosynthesis of building blocks amino acids glutamine
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily, periplasmic binding component

Essentiality data for artI knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Subunit of: predicted ABC transporter ArtPQMI

Subunit composition of predicted ABC transporter ArtPQMI = [ArtI][ArtM][ArtQ][ArtP]2
         periplasmic binding protein ArtI = ArtI (summary available)
         L-arginine ABC transporter - membrane subunit = ArtM (summary available)
         L-arginine ABC transporter - membrane subunit = ArtQ (summary available)
         L-arginine ABC transporter - ATP binding subunit = ArtP (summary available)

The art gene cluster contains two transcription units: artPIQM and artJ [Wissenbach95] which are regulated by the the arginine repressor, ArgR [Caldara06, Caldara07]. Sequence analysis indicates that ArtQ, ArtP and ArtM are the membrane components of an ATP dependent transporter, and ArtJ has been experimentally characterised as an L-arginine binding periplasmic protein. ArtI exhibits homology with a number of ABC transporter periplasmic binding proteins, but its substrate is not known. Purified ArtI does not bind any of the common or uncommon amino acids nor putrescine [Linton98, Wissenbach95]. It is assumed that ArtJ and ArtI are periplasmic binding proteins that share the ArtQPM membrane proteins [Wissenbach95].

Created 07-May-2014 by Mackie A , Macquarie University
Last-Curated ? 08-May-2014 by Mackie A , Macquarie University

Sequence Features

Protein sequence of periplasmic binding protein ArtI with features indicated

Feature Class Location Citations Comment
Signal-Sequence 1 -> 19
[Wissenbach95, Wilkins98, Link97, UniProt11]
Chain 20 -> 243
UniProt: Putative ABC transporter arginine-binding protein 2;
Sequence-Conflict 48
[Wissenbach93, UniProt10]
UniProt: (in Ref. 1);
Sequence-Conflict 86
[Wissenbach93, UniProt10]
UniProt: (in Ref. 1; CAA60102);
Sequence-Conflict 130
[Wissenbach93, UniProt10]
UniProt: (in Ref. 1; CAA60102);
Sequence-Conflict 164
[Wissenbach93, UniProt10]
UniProt: (in Ref. 1; CAA60102);
Sequence-Conflict 179
[Wissenbach93, UniProt10]
UniProt: (in Ref. 1; CAA60102);
Sequence-Conflict 189 -> 190
[Wissenbach93, UniProt10]
UniProt: (in Ref. 1; CAA60102);

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Units:

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram


10/20/97 Gene b0863 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11625; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Caldara06: Caldara M, Charlier D, Cunin R (2006). "The arginine regulon of Escherichia coli: whole-system transcriptome analysis discovers new genes and provides an integrated view of arginine regulation." Microbiology 152(Pt 11);3343-54. PMID: 17074904

Caldara07: Caldara M, Minh PN, Bostoen S, Massant J, Charlier D (2007). "ArgR-dependent Repression of Arginine and Histidine Transport Genes in Escherichia coli K-12." J Mol Biol 373(2);251-67. PMID: 17850814

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Han14: Han MJ, Kim JY, Kim JA (2014). "Comparison of the large-scale periplasmic proteomes of the Escherichia coli K-12 and B strains." J Biosci Bioeng 117(4);437-42. PMID: 24140104

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Link97: Link AJ, Robison K, Church GM (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18(8);1259-313. PMID: 9298646

Linton98: Linton KJ, Higgins CF (1998). "The Escherichia coli ATP-binding cassette (ABC) proteins." Mol Microbiol 1998;28(1);5-13. PMID: 9593292

LopezCampistrou05: Lopez-Campistrous A, Semchuk P, Burke L, Palmer-Stone T, Brokx SJ, Broderick G, Bottorff D, Bolch S, Weiner JH, Ellison MJ (2005). "Localization, annotation, and comparison of the Escherichia coli K-12 proteome under two states of growth." Mol Cell Proteomics 4(8);1205-9. PMID: 15911532

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt11: UniProt Consortium (2011). "UniProt version 2011-06 released on 2011-06-30 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Wilkins98: Wilkins MR, Gasteiger E, Tonella L, Ou K, Tyler M, Sanchez JC, Gooley AA, Walsh BJ, Bairoch A, Appel RD, Williams KL, Hochstrasser DF (1998). "Protein identification with N and C-terminal sequence tags in proteome projects." J Mol Biol 278(3);599-608. PMID: 9600841

Wissenbach93: Wissenbach U, Keck B, Unden G (1993). "Physical map location of the new artPIQMJ genes of Escherichia coli, encoding a periplasmic arginine transport system." J Bacteriol 175(11);3687-8. PMID: 8501075

Wissenbach95: Wissenbach U, Six S, Bongaerts J, Ternes D, Steinwachs S, Unden G (1995). "A third periplasmic transport system for L-arginine in Escherichia coli: molecular characterization of the artPIQMJ genes, arginine binding and transport." Mol Microbiol 1995;17(4);675-86. PMID: 8801422

Other References Related to Gene Regulation

Lacour04: Lacour S, Landini P (2004). "SigmaS-dependent gene expression at the onset of stationary phase in Escherichia coli: function of sigmaS-dependent genes and identification of their promoter sequences." J Bacteriol 186(21);7186-95. PMID: 15489429

Makarova01a: Makarova KS, Mironov AA, Gelfand MS (2001). "Conservation of the binding site for the arginine repressor in all bacterial lineages." Genome Biol 2(4);RESEARCH0013. PMID: 11305941

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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