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Escherichia coli K-12 substr. MG1655 Transporter: 2-dehydro-3-deoxy-D-gluconate:H+ symporter



Gene: kdgT Accession Numbers: EG11869 (EcoCyc), b3909, ECK3902

Regulation Summary Diagram: ?

Summary:
KdgT is a probable 2-keto-3-deoxy-D-gluconate (KDG) uptake system. The cloned kdgT gene increased uptake of KDG and to a lesser extent glucuronate [MandrandBerthel84] and could complement the KDG transport defect in kdgT mutants. KDG transport has been shown in whole cells and membrane vesicles to be via proton symport [Lagarde77]. KdgT is the prototype representative of the KdgT family of KDG transporters. Expression of kdgT is inducible by KDG and is under the control of the KdgR repressor [Pouyssegur74]. Imported KDG is subsequently degraded to pyruvate and triose-phosphate.

Locations: inner membrane

Map Position: [4,099,713 -> 4,100,696] (88.36 centisomes)
Length: 984 bp / 327 aa

Molecular Weight of Polypeptide: 33.669 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0012764 , CGSC:589 , EchoBASE:EB1815 , EcoGene:EG11869 , EcoliWiki:b3909 , OU-Microarray:b3909 , PortEco:kdgT , PR:PRO_000023049 , Protein Model Portal:P0A712 , RefSeq:NP_418345 , RegulonDB:EG11869 , String:511145.b3909 , UniProt:P0A712

Relationship Links: InterPro:IN-FAMILY:IPR004684 , InterPro:IN-FAMILY:IPR018395 , Pfam:IN-FAMILY:PF03812 , ProDom:IN-FAMILY:PD024513

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0035429 - gluconate transmembrane transport Inferred from experiment [MandrandBerthel84]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a]
GO:0008643 - carbohydrate transport Inferred by computational analysis [UniProtGOA11a, GOA06]
GO:0015992 - proton transport Inferred by computational analysis [GOA06]
GO:0046411 - 2-keto-3-deoxygluconate transport Inferred by computational analysis [GOA01a]
Molecular Function: GO:0015649 - 2-keto-3-deoxygluconate:proton symporter activity Inferred from experiment Inferred by computational analysis [GOA06, GOA01a, Lagarde77]
GO:0005351 - sugar:proton symporter activity Inferred by computational analysis [GOA06]
GO:0015293 - symporter activity Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, GOA06, DiazMejia09, Daley05]
GO:0016021 - integral component of membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, GOA01a, MandrandBerthel84]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a]

MultiFun Terms: cell structure membrane
metabolism carbon utilization carbon compounds
transport Electrochemical potential driven transporters Porters (Uni-, Sym- and Antiporters)

Essentiality data for kdgT knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Credits:
Revised 23-May-2011 by Brito D


Enzymatic reaction of: transport of 2-dehydro-3-deoxy-D-gluconate (2-dehydro-3-deoxy-D-gluconate:H+ symporter)

Synonyms: Transport of 2-dehydro-3-deoxy-D-gluconate


Sequence Features

Feature Class Location Citations Comment
Transmembrane-Region 10 -> 30
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 42 -> 62
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 73 -> 93
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 95 -> 115
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 139 -> 159
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 163 -> 183
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 199 -> 219
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 224 -> 244
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 254 -> 274
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 289 -> 309
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
Peter D. Karp on Wed Jan 18, 2006:
Gene left-end position adjusted based on analysis performed in the 2005 E. coli annotation update [Riley06 ].
10/20/97 Gene b3909 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11869; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

GOA06: GOA, SIB (2006). "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Lagarde77: Lagarde AE, Haddock BA (1977). "Proton uptake linked to the 3-deoxy-2-oxo-d-gluconate-transport system of Escherichia coli." Biochem J 1977;162(1);183-7. PMID: 15555

MandrandBerthel84: Mandrand-Berthelot MA, Ritzenthaler P, Mata-Gilsinger M (1984). "Construction and expression of hybrid plasmids containing the structural gene of the Escherichia coli K-12 3-deoxy-2-oxo-D-gluconate transport system." J Bacteriol 1984;160(2);600-6. PMID: 6094479

Pouyssegur74: Pouyssegur J, Stoeber F (1974). "Genetic control of the 2-keto-3-deoxy-d-gluconate metabolism in Escherichia coli K-12: kdg regulon." J Bacteriol 117(2);641-51. PMID: 4359651

Riley06: Riley M, Abe T, Arnaud MB, Berlyn MK, Blattner FR, Chaudhuri RR, Glasner JD, Horiuchi T, Keseler IM, Kosuge T, Mori H, Perna NT, Plunkett G, Rudd KE, Serres MH, Thomas GH, Thomson NR, Wishart D, Wanner BL (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34(1);1-9. PMID: 16397293

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sat Dec 20, 2014, biocyc12.