Escherichia coli K-12 substr. MG1655 Polypeptide: NrfG

Gene: nrfG Accession Numbers: EG11950 (EcoCyc), b4076, ECK4069

Synonyms: yjcN, aidC

Regulation Summary Diagram: ?

Regulation summary diagram for nrfG

NrfG is essential for NrfA activity. It along with NrfE amd NrfF are presumed to be part of a heme lyase that adds heme groups to apocytochrome c 552 [Eaves98].

Gene Citations: [Hussain94]

Locations: periplasmic space

Map Position: [4,291,566 -> 4,292,162] (92.5 centisomes, 333°)
Length: 597 bp / 198 aa

Molecular Weight of Polypeptide: 22.784 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0013354 , CGSC:34345 , EchoBASE:EB1893 , EcoGene:EG11950 , EcoliWiki:b4076 , ModBase:P32712 , OU-Microarray:b4076 , PortEco:nrfG , PR:PRO_000023416 , Protein Model Portal:P32712 , RefSeq:NP_418500 , RegulonDB:EG11950 , SMR:P32712 , String:511145.b4076 , UniProt:P32712

Relationship Links: InterPro:IN-FAMILY:IPR001440 , InterPro:IN-FAMILY:IPR011990 , InterPro:IN-FAMILY:IPR013026 , InterPro:IN-FAMILY:IPR013105 , InterPro:IN-FAMILY:IPR019734 , InterPro:IN-FAMILY:IPR026930 , Panther:IN-FAMILY:PTHR26312:SF27 , Pfam:IN-FAMILY:PF00515 , Pfam:IN-FAMILY:PF07719 , Prosite:IN-FAMILY:PS50005 , Prosite:IN-FAMILY:PS50293

In Paralogous Gene Group: 537 (2 members)

Genetic Regulation Schematic: ?

Genetic regulation schematic for nrfG

GO Terms:

Biological Process: GO:0018378 - cytochrome c-heme linkage via heme-L-cysteine Inferred from experiment [Eaves98]
Cellular Component: GO:0030288 - outer membrane-bounded periplasmic space Inferred by computational analysis [DiazMejia09]

MultiFun Terms: information transfer protein related chaperoning, repair (refolding)
metabolism biosynthesis of macromolecules (cellular constituents) large molecule carriers cytochromes
metabolism energy metabolism, carbon anaerobic respiration

Essentiality data for nrfG knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Sequence Features

Protein sequence of NrfG with features indicated

Feature Class Location Citations Comment
Repeat 73 -> 106
UniProt: TPR 1;
Repeat 144 -> 177
UniProt: TPR 2;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Units:

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram

Transcription-unit diagram


10/20/97 Gene b4076 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11950; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Eaves98: Eaves DJ, Grove J, Staudenmann W, James P, Poole RK, White SA, Griffiths I, Cole JA (1998). "Involvement of products of the nrfEFG genes in the covalent attachment of haem c to a novel cysteine-lysine motif in the cytochrome c552 nitrite reductase from Escherichia coli." Mol Microbiol 28(1);205-16. PMID: 9593308

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

Hussain94: Hussain H, Grove J, Griffiths L, Busby S, Cole J (1994). "A seven-gene operon essential for formate-dependent nitrite reduction to ammonia by enteric bacteria." Mol Microbiol 1994;12(1);153-63. PMID: 8057835

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

Other References Related to Gene Regulation

Browning02: Browning DF, Beatty CM, Wolfe AJ, Cole JA, Busby SJ (2002). "Independent regulation of the divergent Escherichia coli nrfA and acsP1 promoters by a nucleoprotein assembly at a shared regulatory region." Mol Microbiol 43(3);687-701. PMID: 11929525

Browning04: Browning DF, Beatty CM, Sanstad EA, Gunn KE, Busby SJ, Wolfe AJ (2004). "Modulation of CRP-dependent transcription at the Escherichia coli acsP2 promoter by nucleoprotein complexes: anti-activation by the nucleoid proteins FIS and IHF." Mol Microbiol 51(1);241-54. PMID: 14651625

Browning05: Browning DF, Grainger DC, Beatty CM, Wolfe AJ, Cole JA, Busby SJ (2005). "Integration of three signals at the Escherichia coli nrf promoter: a role for Fis protein in catabolite repression." Mol Microbiol 57(2);496-510. PMID: 15978080

Browning06: Browning DF, Lee DJ, Wolfe AJ, Cole JA, Busby SJ (2006). "The Escherichia coli K-12 NarL and NarP proteins insulate the nrf promoter from the effects of integration host factor." J Bacteriol 188(21);7449-56. PMID: 16936015

Browning10: Browning DF, Lee DJ, Spiro S, Busby SJ (2010). "Down-regulation of the Escherichia coli K-12 nrf promoter by binding of the NsrR nitric oxide-sensing transcription repressor to an upstream site." J Bacteriol 192(14);3824-8. PMID: 20472787

Darwin93: Darwin A, Hussain H, Griffiths L, Grove J, Sambongi Y, Busby S, Cole J (1993). "Regulation and sequence of the structural gene for cytochrome c552 from Escherichia coli: not a hexahaem but a 50 kDa tetrahaem nitrite reductase." Mol Microbiol 1993;9(6);1255-65. PMID: 7934939

Darwin97: Darwin AJ, Tyson KL, Busby SJ, Stewart V (1997). "Differential regulation by the homologous response regulators NarL and NarP of Escherichia coli K-12 depends on DNA binding site arrangement." Mol Microbiol 1997;25(3);583-95. PMID: 9302020

Filenko07: Filenko N, Spiro S, Browning DF, Squire D, Overton TW, Cole J, Constantinidou C (2007). "The NsrR regulon of Escherichia coli K-12 includes genes encoding the hybrid cluster protein and the periplasmic, respiratory nitrite reductase." J Bacteriol 189(12):4410-7. PMID: 17449618

Oberto09: Oberto J, Nabti S, Jooste V, Mignot H, Rouviere-Yaniv J (2009). "The HU regulon is composed of genes responding to anaerobiosis, acid stress, high osmolarity and SOS induction." PLoS ONE 4(2);e4367. PMID: 19194530

Page90: Page L, Griffiths L, Cole JA (1990). "Different physiological roles of two independent pathways for nitrite reduction to ammonia by enteric bacteria." Arch Microbiol 1990;154(4);349-54. PMID: 2173895

Partridge09: Partridge JD, Bodenmiller DM, Humphrys MS, Spiro S (2009). "NsrR targets in the Escherichia coli genome: new insights into DNA sequence requirements for binding and a role for NsrR in the regulation of motility." Mol Microbiol 73(4);680-94. PMID: 19656291

Pruss01: Pruss BM, Liu X, Hendrickson W, Matsumura P (2001). "FlhD/FlhC-regulated promoters analyzed by gene array and lacZ gene fusions." FEMS Microbiol Lett 2001;197(1);91-7. PMID: 11287152

Rabin93: Rabin RS, Stewart V (1993). "Dual response regulators (NarL and NarP) interact with dual sensors (NarX and NarQ) to control nitrate- and nitrite-regulated gene expression in Escherichia coli K-12." J Bacteriol 1993;175(11);3259-68. PMID: 8501030

Sclavi07: Sclavi B, Beatty CM, Thach DS, Fredericks CE, Buckle M, Wolfe AJ (2007). "The multiple roles of CRP at the complex acs promoter depend on activation region 2 and IHF." Mol Microbiol 65(2);425-40. PMID: 17630973

Stewart03a: Stewart V, Bledsoe PJ (2003). "Synthetic lac operator substitutions for studying the nitrate- and nitrite-responsive NarX-NarL and NarQ-NarP two-component regulatory systems of Escherichia coli K-12." J Bacteriol 185(7);2104-11. PMID: 12644479

Tyson94: Tyson KL, Cole JA, Busby SJ (1994). "Nitrite and nitrate regulation at the promoters of two Escherichia coli operons encoding nitrite reductase: identification of common target heptamers for both NarP- and NarL-dependent regulation." Mol Microbiol 1994;13(6);1045-55. PMID: 7854119

Wang00: Wang H, Gunsalus RP (2000). "The nrfA and nirB nitrite reductase operons in Escherichia coli are expressed differently in response to nitrate than to nitrite." J Bacteriol 182(20);5813-22. PMID: 11004182

Zhang13: Zhang HK, Lu H, Wang J, Liu GF, Zhou JT, Xu MY (2013). "Global transcriptome analysis of Escherichia coli exposed to immobilized anthraquinone-2-sulfonate and azo dye under anaerobic conditions." Appl Microbiol Biotechnol 97(15);6895-905. PMID: 23820558

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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