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Escherichia coli K-12 substr. MG1655 Enzyme: β-1,6-galactofuranosyltransferase



Gene: wbbI Accession Numbers: EG11983 (EcoCyc), b2034, ECK2028

Synonyms: yefG

Regulation Summary Diagram: ?

Summary:
WbbI is a β-1,6-galactofuranosyltransferase that uses UDP-galactofuranose as the donor and has a modest preference for pyranoside acceptor substrates of the α-D-gluco configuration [Wing06].

When the rfb-50 mutation, an IS5 insertion in the gene encoding rhamnosyl transferase, wbbL, is complemented with a wild type wbbL gene, E. coli K-12 produces the O16 variant of O antigen, with a β-D-galactofuranosyl moiety in the repeating unit [Stevenson94].

Locations: cytosol

Map Position: [2,103,089 <- 2,104,081] (45.33 centisomes)
Length: 993 bp / 330 aa

Molecular Weight of Polypeptide: 37.757 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0006751 , EchoBASE:EB1926 , EcoGene:EG11983 , EcoliWiki:b2034 , OU-Microarray:b2034 , PortEco:wbbI , Pride:P37749 , Protein Model Portal:P37749 , RefSeq:NP_416538 , RegulonDB:EG11983 , String:511145.b2034 , UniProt:P37749

Relationship Links: InterPro:IN-FAMILY:IPR016503

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0009103 - lipopolysaccharide biosynthetic process Inferred by computational analysis [UniProtGOA12, UniProtGOA11a]
GO:0009243 - O antigen biosynthetic process
Molecular Function: GO:0008921 - lipopolysaccharide-1,6-galactosyltransferase activity Inferred from experiment [Wing06]
GO:0016740 - transferase activity Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005737 - cytoplasm Inferred by computational analysis [UniProtGOA11, UniProtGOA11a]

MultiFun Terms: metabolism biosynthesis of macromolecules (cellular constituents) lipopolysaccharide O antigen

Essentiality data for wbbI knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Enzymatic reaction of: β-1,6-galactofuranosyltransferase

EC Number: 2.4.1.-

octyl α-D-glucopyranoside + UDP-α-D-galactofuranose <=> octyl β-1,6-D-galactofuranosyl-α-D-glucopyranoside + UDP + H+

The reaction direction shown, that is, A + B ↔ C + D versus C + D ↔ A + B, is in accordance with the direction in which it was curated.

Reversibility of this reaction is unspecified.

Alternative Substrates for octyl α-D-glucopyranoside: octyl α-D-galactopyranoside [Wing06 ] , octyl α-D-mannopyranoside [Wing06 ] , octyl 2-acetamido-2-deoxy-α-D-glucopyranoside [Wing06 ]


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b2034 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG11983; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Stevenson94: Stevenson G, Neal B, Liu D, Hobbs M, Packer NH, Batley M, Redmond JW, Lindquist L, Reeves P (1994). "Structure of the O antigen of Escherichia coli K-12 and the sequence of its rfb gene cluster." J Bacteriol 1994;176(13);4144-56. PMID: 7517391

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA12: UniProt-GOA (2012). "Gene Ontology annotation based on UniPathway vocabulary mapping."

Wing06: Wing C, Errey JC, Mukhopadhyay B, Blanchard JS, Field RA (2006). "Expression and initial characterization of WbbI, a putative D-Galf:alpha-D-Glc beta-1,6-galactofuranosyltransferase from Escherichia coli K-12." Org Biomol Chem 4(21);3945-50. PMID: 17047874


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Sat Nov 22, 2014, BIOCYC13A.