Escherichia coli K-12 substr. MG1655 Polypeptide: peptide ABC transporter - membrane subunit

Gene: yejE Accession Numbers: EG12040 (EcoCyc), b2179, ECK2173

Regulation Summary Diagram: ?

Regulation summary diagram for yejE

Component of: peptide ABC transporter YejABEF (summary available)

YejE is an integral membrane component of the YejA/YejB/YejE/YejF ABC transporter.

Gene Citations: [Antal05]

Locations: inner membrane

Map Position: [2,273,295 -> 2,274,320] (49.0 centisomes, 176°)
Length: 1026 bp / 341 aa

Molecular Weight of Polypeptide: 38.092 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0007210 , DIP:DIP-11935N , EchoBASE:EB1974 , EcoGene:EG12040 , EcoliWiki:b2179 , OU-Microarray:b2179 , PortEco:yejE , Pride:P33915 , Protein Model Portal:P33915 , RefSeq:NP_416684 , RegulonDB:EG12040 , String:511145.b2179 , UniProt:P33915

Relationship Links: InterPro:IN-FAMILY:IPR000515 , Pfam:IN-FAMILY:PF00528 , Prosite:IN-FAMILY:PS50928

In Paralogous Gene Group: 24 (43 members)

Gene-Reaction Schematic: ?

Gene-Reaction Schematic

GO Terms:

Biological Process: GO:0042884 - microcin transport Inferred from experiment [Novikova07]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0008152 - metabolic process Inferred by computational analysis [Saurin99]
Molecular Function: GO:0043492 - ATPase activity, coupled to movement of substances Inferred by computational analysis [Saurin99]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a]
GO:0043190 - ATP-binding cassette (ABC) transporter complex Inferred by computational analysis [Saurin99]

MultiFun Terms: cell structure membrane
transport Channel-type Transporters Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases ABC superfamily, membrane component

Essentiality data for yejE knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Subunit of: peptide ABC transporter YejABEF

Subunit composition of peptide ABC transporter YejABEF = [YejF]2[YejB][YejE][YejA]
         peptide ABC transporter - ATP binding subunit = YejF (summary available)
         peptide ABC transporter - membrane subunit = YejB (summary available)
         peptide ABC transporter - membrane subunit = YejE (summary available)
         peptide ABC transporter - periplasmic binding protein = YejA (summary available)

YejF, YejB, YejE and YejA are members of the ABC superfamily of transporters [Saurin99]. YejF is the ATP-binding protein, YejB and YejE are the membrane proteins, and YejA is the periplasmic binding protein. Based on sequence similarity these proteins probably function together as an ATP-dependent oligopeptide permease. The yejF, yejB, yejE, and yejA genes are probably in a single operon.

yejA, yejB, yejE, and yejF mutants are resistant to Microcin C due to a deficiency in uptake [Novikova07].

Expression yejABEF appears to be regulated by the small RNA RydC at the mRNA level [Antal05].

Citations: [Serres05, Khan02]

Last-Curated ? 02-Jan-2008 by Johnson A , JCVI

Enzymatic reaction of: Transport of a peptide (peptide ABC transporter YejABEF)

Transport reaction diagram for Transport of a peptide

Alternative Products for a peptide: Microcin C [Novikova07 ]

Sequence Features

Protein sequence of peptide ABC transporter - membrane subunit with features indicated

Feature Class Location Citations Comment
Transmembrane-Region 22 -> 42
UniProt: Helical;; Non-Experimental Qualifier: potential;
Conserved-Region 140 -> 332
UniProt: ABC transmembrane type-1;
Transmembrane-Region 144 -> 164
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 179 -> 199
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 202 -> 222
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 253 -> 273
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 308 -> 328
UniProt: Helical;; Non-Experimental Qualifier: potential;

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


10/20/97 Gene b2179 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12040; confirmed by SwissProt match.


Antal05: Antal M, Bordeau V, Douchin V, Felden B (2005). "A small bacterial RNA regulates a putative ABC transporter." J Biol Chem 280(9);7901-8. PMID: 15618228

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Khan02: Khan MA, Isaacson RE (2002). "Identification of Escherichia coli genes that are specifically expressed in a murine model of septicemic infection." Infect Immun 70(7);3404-12. PMID: 12065479

Novikova07: Novikova M, Metlitskaya A, Datsenko K, Kazakov T, Kazakov A, Wanner B, Severinov K (2007). "The Escherichia coli Yej transporter is required for the uptake of translation inhibitor microcin C." J Bacteriol 189(22);8361-5. PMID: 17873039

Saurin99: Saurin W, Hofnung M, Dassa E (1999). "Getting in or out: early segregation between importers and exporters in the evolution of ATP-binding cassette (ABC) transporters." J Mol Evol 1999;48(1);22-41. PMID: 9873074

Serres05: Serres MH, Riley M (2005). "Gene fusions and gene duplications: relevance to genomic annotation and functional analysis." BMC Genomics 6(1);33. PMID: 15757509

UniProt09: UniProt Consortium (2009). "UniProt version 15.8 released on 2009-10-01 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
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