Escherichia coli K-12 substr. MG1655 Polypeptide: ferrous iron transport protein A

Gene: feoA Accession Numbers: EG12101 (EcoCyc), b3408, ECK3395

Regulation Summary Diagram: ?

Regulation summary diagram for feoA

A feoA::Tn5 mutant shows partially reduced uptake of ferrous iron [Kammler93].

Overexpression of feoA increases the tolerance of E. coli to n-butanol [Reyes11].

FeoA: "ferrous iron uptake system"

Map Position: [3,538,185 -> 3,538,412] (76.26 centisomes, 275°)
Length: 228 bp / 75 aa

Molecular Weight of Polypeptide: 8.371 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0011124 , CGSC:28964 , EchoBASE:EB2025 , EcoGene:EG12101 , EcoliWiki:b3408 , OU-Microarray:b3408 , PortEco:feoA , PR:PRO_000022597 , Protein Model Portal:P0AEL3 , RefSeq:NP_417867 , RegulonDB:EG12101 , SMR:P0AEL3 , String:511145.b3408 , UniProt:P0AEL3

Relationship Links: InterPro:IN-FAMILY:IPR007167 , InterPro:IN-FAMILY:IPR008988 , PDB:Structure:2GCX , PDB:Structure:2LX9 , Pfam:IN-FAMILY:PF04023 , Smart:IN-FAMILY:SM00899

Genetic Regulation Schematic: ?

Genetic regulation schematic for feoA

GO Terms:

Biological Process: GO:0006974 - cellular response to DNA damage stimulus Inferred from experiment [Khil02]
GO:0070627 - ferrous iron import Inferred from experiment [Kammler93]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
GO:0006811 - ion transport Inferred by computational analysis [UniProtGOA11]
GO:0055072 - iron ion homeostasis Inferred by computational analysis [UniProtGOA11]
Molecular Function: GO:0046914 - transition metal ion binding Inferred by computational analysis [GOA01]

MultiFun Terms: cell processes adaptations Fe aquisition
transport Transporters of Unknown Classification

Essentiality data for feoA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Last-Curated ? 13-Oct-2011 by Keseler I , SRI International

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Units:

Transcription-unit diagram

Transcription-unit diagram


10/20/97 Gene b3408 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12101; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Hantke03: Hantke K (2003). "Is the bacterial ferrous iron transporter FeoB a living fossil?." Trends Microbiol 11(5);192-5. PMID: 12781516

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Kammler93: Kammler M, Schon C, Hantke K (1993). "Characterization of the ferrous iron uptake system of Escherichia coli." J Bacteriol 1993;175(19);6212-9. PMID: 8407793

Khil02: Khil PP, Camerini-Otero RD (2002). "Over 1000 genes are involved in the DNA damage response of Escherichia coli." Mol Microbiol 44(1);89-105. PMID: 11967071

Reyes11: Reyes LH, Almario MP, Kao KC (2011). "Genomic library screens for genes involved in n-butanol tolerance in Escherichia coli." PLoS One 6(3);e17678. PMID: 21408113

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

Other References Related to Gene Regulation

Cartron06: Cartron ML, Maddocks S, Gillingham P, Craven CJ, Andrews SC (2006). "Feo--transport of ferrous iron into bacteria." Biometals 19(2);143-57. PMID: 16718600

Huerta03: Huerta AM, Collado-Vides J (2003). "Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals." J Mol Biol 333(2);261-78. PMID: 14529615

Oberto10: Oberto J (2010). "FITBAR: a web tool for the robust prediction of prokaryotic regulons." BMC Bioinformatics 11;554. PMID: 21070640

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Tue Oct 13, 2015, BIOCYC11A.