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Escherichia coli K-12 substr. MG1655 Transporter: threonine / homoserine efflux transporter



Gene: rhtA Accession Numbers: EG12134 (EcoCyc), b0813, ECK0802

Synonyms: ybiF

Regulation Summary Diagram: ?

Summary:
RhtA is a threonine/homoserine exporter in the Drug/Metabolite Transporter superfamily (TC: 2.A.7). It is homologous to EamA, an exporter that translocates metabolites of the cysteine pathway of E. coli. RhtA has 10 putative transmembrane spanning alpha helices. RhtA may also export other amino acids such as proline, serine, cysteine, and histidine based upon resistance phenotypes. [Livshits03]

Locations: inner membrane

Map Position: [848,433 <- 849,320] (18.29 centisomes)
Length: 888 bp / 295 aa

Molecular Weight of Polypeptide: 31.168 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0002779 , EchoBASE:EB2055 , EcoGene:EG12134 , EcoliWiki:b0813 , OU-Microarray:b0813 , PortEco:rhtA , PR:PRO_000023747 , Protein Model Portal:P0AA67 , RefSeq:NP_415334 , RegulonDB:EG12134 , String:511145.b0813 , UniProt:P0AA67

Relationship Links: InterPro:IN-FAMILY:IPR000620 , Pfam:IN-FAMILY:PF00892

In Paralogous Gene Group: 309 (5 members)

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0015826 - threonine transport Inferred from experiment [Livshits03]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11a]
GO:0006865 - amino acid transport Inferred by computational analysis [UniProtGOA11a]
Molecular Function: GO:0015565 - threonine efflux transmembrane transporter activity Inferred from experiment [Livshits03]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11, UniProtGOA11a, DiazMejia09, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11a, GOA01a]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11a]

MultiFun Terms: cell structure membrane
transport Electrochemical potential driven transporters Porters (Uni-, Sym- and Antiporters)

Essentiality data for rhtA knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Enzymatic reaction of: homoserine efflux transporter


Enzymatic reaction of: L-threonine efflux transporter (threonine / homoserine efflux transporter)


Sequence Features

Feature Class Location Citations Comment
Transmembrane-Region 10 -> 30
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Conserved-Region 30 -> 135
[UniProt14]
UniProt: EamA 1.
Transmembrane-Region 39 -> 59
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 72 -> 92
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 94 -> 114
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 119 -> 139
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 147 -> 167
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Conserved-Region 159 -> 278
[UniProt14]
UniProt: EamA 2.
Transmembrane-Region 176 -> 196
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 201 -> 221
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 238 -> 258
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 263 -> 283
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Sequence-Conflict 268
[Lomovskaya94, UniProt10a]
Alternate sequence: A → R; UniProt: (in Ref. 1; AAA21854);


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b0813 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12134; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01a: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Livshits03: Livshits VA, Zakataeva NP, Aleshin VV, Vitushkina MV (2003). "Identification and characterization of the new gene rhtA involved in threonine and homoserine efflux in Escherichia coli." Res Microbiol 154(2);123-35. PMID: 12648727

Lomovskaya94: Lomovskaya OL, Kidwell JP, Matin A (1994). "Characterization of the sigma 38-dependent expression of a core Escherichia coli starvation gene, pexB." J Bacteriol 176(13);3928-35. PMID: 8021175

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProt14: UniProt Consortium (2014). "UniProt version 2014-01 released on 2014-01-01 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Wed Dec 17, 2014, BIOCYC14B.