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Escherichia coli K-12 substr. MG1655 Transporter: muropeptide:H+ symporter



Gene: ampG Accession Numbers: EG12183 (EcoCyc), b0433, ECK0427

Regulation Summary Diagram: ?

Summary:
AmpG is a member of the major facilitator superfamily of transporters, and together with AmpD, is essential for induction of the AmpC Β-lactamase and is involved in the recycling of cell wall peptides [Korfmann89, Lindquist93, Schmidt95, Park95c]. Mutants in ampG are unable to induce ampC and display greatly increased cell wall turnover [Jacobs94]. AmpG is responsible for the transport of precursors of the anhMurNAc tripeptide into the cytoplasm [Dietz96]. These precursors are the products of peptidoglycan degradation and include the disaccharide GlcNAc-anhMurNAc as well as GlcNAc-anhMurNAc-oligopeptides (tri-, tetra-, and pentapeptides). Transport is dependent on the proton motive force [Cheng02a]. Following uptake of these muropeptides, they are degraded, releasing the components which can subsequently be used in cell wall synthesis [Jacobs95]. Experiments with β-lactamase fusions show AmpG contains two large cytoplasmic loops and 10 transmembrane segments [Chahboune05]. Cytosolic muramyl peptides probably induce expression of ampC by binding to its regulator AmpR [Normark95].

Review: [Park08]

Locations: inner membrane

Map Position: [451,294 <- 452,769] (9.73 centisomes)
Length: 1476 bp / 491 aa

Molecular Weight of Polypeptide: 53.245 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0001501 , CGSC:31027 , EchoBASE:EB2100 , EcoGene:EG12183 , EcoliWiki:b0433 , ModBase:P0AE16 , OU-Microarray:b0433 , PortEco:ampG , PR:PRO_000022098 , Protein Model Portal:P0AE16 , RefSeq:NP_414967 , RegulonDB:EG12183 , SMR:P0AE16 , String:511145.b0433 , UniProt:P0AE16

Relationship Links: InterPro:IN-FAMILY:IPR004752 , InterPro:IN-FAMILY:IPR011701 , InterPro:IN-FAMILY:IPR016196 , InterPro:IN-FAMILY:IPR020846 , Pfam:IN-FAMILY:PF07690 , Prosite:IN-FAMILY:PS50850

In Paralogous Gene Group: 127 (2 members)

Gene-Reaction Schematic: ?

GO Terms:

Biological Process: GO:0000270 - peptidoglycan metabolic process Inferred from experiment [Cheng02a]
GO:0015835 - peptidoglycan transport Inferred from experiment [Cheng02a]
GO:0015992 - proton transport Inferred from experiment [Cheng02a]
GO:0006810 - transport Inferred by computational analysis [UniProtGOA11]
GO:0055085 - transmembrane transport Inferred by computational analysis [GOA01]
Molecular Function: GO:0015295 - solute:proton symporter activity Inferred from experiment [Cheng02a]
Cellular Component: GO:0005886 - plasma membrane Inferred from experiment Inferred by computational analysis [UniProtGOA11a, UniProtGOA11, DiazMejia09, Daley05]
GO:0016020 - membrane Inferred by computational analysis [UniProtGOA11, GOA01]
GO:0016021 - integral component of membrane Inferred by computational analysis [UniProtGOA11, GOA01]

MultiFun Terms: cell structure membrane
cell structure murein
metabolism central intermediary metabolism murein turnover, recycling
transport Electrochemical potential driven transporters Porters (Uni-, Sym- and Antiporters)

Essentiality data for ampG knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]
Yes [Feist07, Comment 4]

Enzymatic reaction of: transport of N-acetyl-β-D-glucosamine(anhydrous)-N-acetylmuramate (muropeptide:H+ symporter)


Enzymatic reaction of: transport of GlcNAc-1,6-anhMurNAc-L-Ala-γ-D-Glu-DAP-D-Ala (muropeptide:H+ symporter)


Sequence Features

Feature Class Location Citations Comment
Transmembrane-Region 13 -> 33
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 81 -> 105
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 110 -> 128
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Extrinsic-Sequence-Variant 151
[UniProt10a]
Alternate sequence: G → D; UniProt: (in non-inducible mutant SN0301- 1);
Transmembrane-Region 174 -> 197
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 224 -> 244
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 266 -> 283
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Extrinsic-Sequence-Variant 268
[UniProt10a]
Alternate sequence: G → D; UniProt: (in non-inducible mutant SN0301- 3);
Transmembrane-Region 328 -> 345
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Extrinsic-Sequence-Variant 373
[UniProt10a]
Alternate sequence: G → D; UniProt: (in non-inducible mutant SN0301- 5);
Transmembrane-Region 382 -> 406
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 425 -> 450
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;
Transmembrane-Region 461 -> 485
[UniProt10]
UniProt: Helical;; Non-Experimental Qualifier: potential;


Gene Local Context (not to scale): ?

Transcription Unit:

Notes:

History:
10/20/97 Gene b0433 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12183; confirmed by SwissProt match.


References

Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Chahboune05: Chahboune A, Decaffmeyer M, Brasseur R, Joris B (2005). "Membrane topology of the Escherichia coli AmpG permease required for recycling of cell wall anhydromuropeptides and AmpC beta-lactamase induction." Antimicrob Agents Chemother 49(3);1145-9. PMID: 15728916

Cheng02a: Cheng Q, Park JT (2002). "Substrate specificity of the AmpG permease required for recycling of cell wall anhydro-muropeptides." J Bacteriol 184(23);6434-6. PMID: 12426329

Daley05: Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308(5726);1321-3. PMID: 15919996

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Dietz96: Dietz H, Pfeifle D, Wiedemann B (1996). "Location of N-acetylmuramyl-L-alanyl-D-glutamylmesodiaminopimelic acid, presumed signal molecule for beta-lactamase induction, in the bacterial cell." Antimicrob Agents Chemother 40(9);2173-7. PMID: 8878601

Feist07: Feist AM, Henry CS, Reed JL, Krummenacker M, Joyce AR, Karp PD, Broadbelt LJ, Hatzimanikatis V, Palsson BO (2007). "A genome-scale metabolic reconstruction for Escherichia coli K-12 MG1655 that accounts for 1260 ORFs and thermodynamic information." Mol Syst Biol 3;121. PMID: 17593909

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

GOA01: GOA, DDB, FB, MGI, ZFIN (2001). "Gene Ontology annotation through association of InterPro records with GO terms."

Jacobs94: Jacobs C, Huang LJ, Bartowsky E, Normark S, Park JT (1994). "Bacterial cell wall recycling provides cytosolic muropeptides as effectors for beta-lactamase induction." EMBO J 1994;13(19);4684-94. PMID: 7925310

Jacobs95: Jacobs C, Joris B, Jamin M, Klarsov K, Van Beeumen J, Mengin-Lecreulx D, van Heijenoort J, Park JT, Normark S, Frere JM (1995). "AmpD, essential for both beta-lactamase regulation and cell wall recycling, is a novel cytosolic N-acetylmuramyl-L-alanine amidase." Mol Microbiol 1995;15(3);553-9. PMID: 7783625

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Korfmann89: Korfmann G, Sanders CC (1989). "ampG is essential for high-level expression of AmpC beta-lactamase in Enterobacter cloacae." Antimicrob Agents Chemother 1989;33(11);1946-51. PMID: 2692514

Lindquist93: Lindquist S, Weston-Hafer K, Schmidt H, Pul C, Korfmann G, Erickson J, Sanders C, Martin HH, Normark S (1993). "AmpG, a signal transducer in chromosomal beta-lactamase induction." Mol Microbiol 1993;9(4);703-15. PMID: 8231804

Normark95: Normark S (1995). "beta-Lactamase induction in gram-negative bacteria is intimately linked to peptidoglycan recycling." Microb Drug Resist 1995;1(2);111-4. PMID: 9158742

Park08: Park JT, Uehara T (2008). "How bacteria consume their own exoskeletons (turnover and recycling of cell wall peptidoglycan)." Microbiol Mol Biol Rev 72(2);211-27, table of contents. PMID: 18535144

Park95c: Park JT (1995). "Why does Escherichia coli recycle its cell wall peptides?." Mol Microbiol 1995;17(3);421-6. PMID: 8559061

Schmidt95: Schmidt H, Korfmann G, Barth H, Martin HH (1995). "The signal transducer encoded by ampG is essential for induction of chromosomal AmpC beta-lactamase in Escherichia coli by beta-lactam antibiotics and 'unspecific' inducers." Microbiology 1995;141 ( Pt 5);1085-92. PMID: 7773404

UniProt10: UniProt Consortium (2010). "UniProt version 2010-07 released on 2010-06-15 00:00:00." Database.

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

UniProtGOA11: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on the manual assignment of UniProtKB Subcellular Location terms in UniProtKB/Swiss-Prot entries."


Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 18.5 on Mon Nov 24, 2014, BIOCYC13B.