Escherichia coli K-12 substr. MG1655 Polypeptide: putative membrane protein

Gene: yacH Accession Numbers: EG12315 (EcoCyc), b0117, ECK0116

Regulation Summary Diagram: ?

Regulation summary diagram for yacH

A yacH mutant has been constructed [Merlin02a]. YacH, a predicted membrane protein, has reduced expression upon exposure to a sublethal dose of the cationic antimicrobial insect peptide cecropin A [Hong03].

Map Position: [129,407 <- 131,260] (2.79 centisomes, 10°)
Length: 1854 bp / 617 aa

Molecular Weight of Polypeptide: 69.362 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0000406 , EchoBASE:EB2221 , EcoGene:EG12315 , EcoliWiki:b0117 , OU-Microarray:b0117 , PortEco:yacH , Pride:P36682 , Protein Model Portal:P36682 , RefSeq:NP_414659 , RegulonDB:EG12315 , String:511145.b0117 , UniProt:P36682

Relationship Links: InterPro:IN-FAMILY:IPR021728 , Pfam:IN-FAMILY:PF11737

MultiFun Terms: cell structure membrane

Essentiality data for yacH knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB enriched Yes 37 Aerobic 6.95   Yes [Gerdes03, Comment 1]
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 2]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 3]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 2]

Sequence Features

Protein sequence of putative membrane protein with features indicated

Feature Class Location Citations Comment
Sequence-Conflict 523
[Fujita94, UniProt10a]
UniProt: (in Ref. 1);

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Unit:

Transcription-unit diagram


10/20/97 Gene b0117 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12315; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

Fujita94: Fujita N, Mori H, Yura T, Ishihama A (1994). "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region." Nucleic Acids Res 22(9);1637-9. PMID: 8202364

Gerdes03: Gerdes SY, Scholle MD, Campbell JW, Balazsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabasi AL, Oltvai ZN, Osterman AL (2003). "Experimental determination and system level analysis of essential genes in Escherichia coli MG1655." J Bacteriol 185(19);5673-84. PMID: 13129938

Hong03: Hong RW, Shchepetov M, Weiser JN, Axelsen PH (2003). "Transcriptional profile of the Escherichia coli response to the antimicrobial insect peptide cecropin A." Antimicrob Agents Chemother 47(1);1-6. PMID: 12499161

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Merlin02a: Merlin C, McAteer S, Masters M (2002). "Tools for characterization of Escherichia coli genes of unknown function." J Bacteriol 184(16);4573-81. PMID: 12142427

UniProt10a: UniProt Consortium (2010). "UniProt version 2010-11 released on 2010-11-02 00:00:00." Database.

Report Errors or Provide Feedback
Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Tue Oct 6, 2015, biocyc13.