Escherichia coli K-12 substr. MG1655 Polypeptide: predicted cation transport regulator

Gene: chaB Accession Numbers: EG12402 (EcoCyc), b1217, ECK1211

Regulation Summary Diagram: ?

Regulation summary diagram for chaB

ChaB is predicted to be a regulator of cation transport. The solution structure of ChaB shows a fold also found in the cyclin-box family of proteins [Osborne04].

Gene Citations: [Ivey93]

Locations: cytosol

Map Position: [1,271,342 -> 1,271,572] (27.4 centisomes, 99°)
Length: 231 bp / 76 aa

Molecular Weight of Polypeptide: 8.945 kD (from nucleotide sequence)

Unification Links: ASAP:ABE-0004087 , CGSC:37193 , DIP:DIP-47930N , EchoBASE:EB2302 , EcoGene:EG12402 , EcoliWiki:b1217 , OU-Microarray:b1217 , PortEco:chaB , PR:PRO_000022270 , Protein Model Portal:P0AE63 , RefSeq:NP_415735 , RegulonDB:EG12402 , SMR:P0AE63 , String:511145.b1217 , Swiss-Model:P0AE63 , UniProt:P0AE63

Relationship Links: InterPro:IN-FAMILY:IPR009317 , PDB:Structure:1SG7 , Pfam:IN-FAMILY:PF06150

GO Terms:

Biological Process: GO:0006351 - transcription, DNA-templated Inferred by computational analysis [UniProtGOA11a]
GO:0006355 - regulation of transcription, DNA-templated Inferred by computational analysis [UniProtGOA11a]
Cellular Component: GO:0005737 - cytoplasm
GO:0005829 - cytosol Inferred by computational analysis [DiazMejia09]

MultiFun Terms: regulation

Essentiality data for chaB knockouts: ?

Growth Medium Growth? T (°C) O2 pH Osm/L Growth Observations
LB Lennox Yes 37 Aerobic 7   Yes [Baba06, Comment 1]
M9 medium with 1% glycerol Yes 37 Aerobic 7.2 0.35 Yes [Joyce06, Comment 2]
MOPS medium with 0.4% glucose Yes 37 Aerobic 7.2 0.22 Yes [Baba06, Comment 1]

Last-Curated ? 12-Jan-2006 by Keseler I , SRI International

Gene Local Context (not to scale): ?

Gene local context diagram

Transcription Units:

Transcription-unit diagram

Transcription-unit diagram


10/20/97 Gene b1217 from Blattner lab Genbank (v. M52) entry merged into EcoCyc gene EG12402; confirmed by SwissProt match.


Baba06: Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006). "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection." Mol Syst Biol 2;2006.0008. PMID: 16738554

DiazMejia09: Diaz-Mejia JJ, Babu M, Emili A (2009). "Computational and experimental approaches to chart the Escherichia coli cell-envelope-associated proteome and interactome." FEMS Microbiol Rev 33(1);66-97. PMID: 19054114

Ivey93: Ivey DM, Guffanti AA, Zemsky J, Pinner E, Karpel R, Padan E, Schuldiner S, Krulwich TA (1993). "Cloning and characterization of a putative Ca2+/H+ antiporter gene from Escherichia coli upon functional complementation of Na+/H+ antiporter-deficient strains by the overexpressed gene." J Biol Chem 1993;268(15);11296-303. PMID: 8496184

Joyce06: Joyce AR, Reed JL, White A, Edwards R, Osterman A, Baba T, Mori H, Lesely SA, Palsson BO, Agarwalla S (2006). "Experimental and computational assessment of conditionally essential genes in Escherichia coli." J Bacteriol 188(23);8259-71. PMID: 17012394

Osborne04: Osborne MJ, Siddiqui N, Iannuzzi P, Gehring K (2004). "The solution structure of ChaB, a putative membrane ion antiporter regulator from Escherichia coli." BMC Struct Biol 4;9. PMID: 15306028

UniProtGOA11a: UniProt-GOA (2011). "Gene Ontology annotation based on manual assignment of UniProtKB keywords in UniProtKB/Swiss-Prot entries."

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Please cite the following article in publications resulting from the use of EcoCyc: Nucleic Acids Research 41:D605-12 2013
Page generated by SRI International Pathway Tools version 19.0 on Sat Oct 3, 2015, biocyc13.